Seed Coating/Seed Treatment: Prepare a mixture of 10 - 15 grams of Acidithiobacillus Ferrooxidans in a sufficient amount of water to create a slurry. Coat 1kg of seeds with the mixture, dry them in shade, and they will be ready to use in the field.

Seedling Treatment: Prepare a mixture of 100 grams of Acidithiobacillus Ferrooxidans in a sufficient amount of water. Dip the roots of the seedlings into the solution for 30 minutes to allow the bacteria to attach to the roots prior to planting.

Soil Treatment: Mix 2.5 - 5kg per hectare of Acidithiobacillus Ferrooxidans with organic manure/organic fertilizers. Incorporate the mixture into the soil and spread it in the field at the time of planting/sowing.

Irrigation: Mix 2.5 - 5kg per hectare of Acidithiobacillus Ferrooxidans in a sufficient amount of water. Drench or drip the mixture to penetrate the root zones.