< Animal Health Pastocare Formulated just for sheep and goats, Pasto Care contains molybdenum to prevent copper toxicity and it is made without any added copper. Higher levels of zinc and iron are important for maintaining high growth rates, preventing anemia, and increasing resistance to disease. To prevent goiter and to aid in improvement of reproductive efficiency, Iodine is included at the correct level. Product Enquiry Benefits Protects Against Bacterial Infections Helps prevent infections caused by E. coli and Salmonella, safeguarding animal health. Improves Fertility and Reproductive Health Supports better fertility and contributes to overall reproductive efficiency in livestock. Promotes Rapid Weight Gain and Growth Enhances body weight increase and supports faster development for improved productivity. Boosts Immunity and Recovery Aids in recovering from vitamin deficiencies, strengthens the immune system, and improves survival and performance. Component Each kg contains Vitamin A 250,000 I.U. Vitamin D3 25,000 I.U. Vitamin E 5,000 I.U. Lactobacillus Acidophilus 2 billion CFU Lactobacillus Fermentum 2 billion CFU Lactobacillus Bifidus 2 billion CFU Mineral Mineral Ferrous Sulphate Potassium Iodate Zinc Oxide Cobalt Sulphate Manganous Oxide Sodium Selenite Magnesium Oxide Composition Dosage & Application Additional Info Dosage & Application Content coming soon! Additional Info Content coming soon! Related Products Stress Pro Camel Care Pro Cattle Care Max Cattle Care Pro Feed Pro Grass Mask Lactomine Pro Lactomix Mineral Max Calf Pro More Products Resources Read all

< Animal Health Cattle Care Max Cattle Care Max is a powerful probiotic blend formulated for cattle. It aids in greater weight gain, faster growth, improved fertility and a increase in milk production. Cattle Care Max will improve the gut-health of the animals by boosting immunity and preventing diseases. Product Enquiry Benefits Supports Livestock During Stress Effective during periods of stress such as transport, climate changes, or illness, helping to stabilize health and recovery. Boosts Immunity and Overall Health Strengthens the immune system and supports general health, making cattle more resilient to infections and disease. Promotes Weight Gain and Accelerated Growth Supports steady weight gain and enhances growth rate, leading to better physical development in cattle. Enhances Fertility and Milk Yield Improves reproductive performance and boosts milk production, contributing to higher productivity in dairy and breeding cattle. Component Amount per kg Bacillus Subtilis 3 × 10⁹ Amylase 24,000 B.A.U. Protease 18,000 casein digest. units Hemicellulase 3,000 units Lipase 70,000 units Beta Glucanase 12,000 Beta Glucanase units Phytase 50,000 units Extract from Bacillus Subtilis fermentation (dehydrated) 15% Composition Dosage & Application Additional Info Dosage & Application Content coming soon! Additional Info Content coming soon! Related Products Stress Pro Camel Care Pro Cattle Care Pro Feed Pro Grass Mask Lactomine Pro Lactomix Mineral Max Pastocare Calf Pro More Products Resources Read all

Bacillus circulans produces indoleacetic acid, solubilizes phosphorus improving absorption, enhances plant growth and yield, safe and eco-friendly. < Microbial Species Bacillus circulans Bacillus circulans produces indoleacetic acid, solubilizes phosphorus improving absorption, enhances plant growth and yield, safe and eco-friendly. Strength 1 x 10⁸ CFU per gram / 1 x 10⁹ CFU per gram Product Enquiry Download Brochure Benefits Enhances nutrient availability in soil Bacillus circulans improves the availability of essential nutrients such as nitrogen, phosphorus, and potassium, promoting better plant growth. Improves phosphorus uptake by plants Facilitates the solubilization of insoluble phosphates in the soil, making phosphorus more accessible to plants for optimal growth and development. Promotes plant growth and development Produces indoleacetic acid (IAA), a plant growth hormone that enhances root and shoot development, resulting in healthier and more vigorous plants. Enhances resistance to abiotic stress Increases plant tolerance to environmental stressors such as drought, salinity, and temperature fluctuations, improving overall plant resilience. Dosage & Application Additional Info Scientific References Mode of Action FAQ Scientific References Content coming soon! Mode of Action Introduction to Bacillus circulans: Nature's Multi-Functional Plant Growth Enhancer Bacillus circulans is a versatile Gram-positive, endospore-forming bacterium that has earned recognition as one of the most effective plant growth-promoting rhizobacteria (PGPR) in sustainable agriculture. Originally described in 1890 by Jordan, this remarkable microorganism demonstrates exceptional abilities in phosphate solubilization, indole-3-acetic acid (IAA) production, and comprehensive plant health enhancement. Recent taxonomic reclassifications have established that Bacillus circulans is now properly classified as Niallia circulans, reflecting advances in bacterial systematics and phylogenetic analysis. mdpi+4 This beneficial bacterium thrives in diverse soil environments and establishes mutually beneficial relationships with numerous crop species, making it an invaluable component of modern biofertilizer formulations. The organism's unique combination of growth-promoting metabolites, stress tolerance capabilities, and environmental safety positions it as a critical tool for addressing agricultural challenges while promoting ecological sustainability. pubmed.ncbi.nlm.nih+1 Characteristics and Biology of Bacillus circulans Taxonomic Classification and Modern Nomenclature Bacillus circulans has undergone significant taxonomic revision in recent years. Comprehensive phylogenetic analyses using 16S rRNA sequencing and comparative genomics have led to its reclassification as Niallia circulans (Jordan 1890) Gupta et al. 2020. This reclassification reflects the polyphyletic nature of the original Bacillus genus and efforts to create more accurate taxonomic groupings based on evolutionary relationships. gbif+1 The genus Niallia was established to honor Professor Niall A. Logan of Glasgow Caledonian University for his contributions to Bacillus systematics. Members of this genus are facultatively anaerobic, motile, and produce resistant endospores, with optimal growth temperatures ranging from 30-37°C. wikipedia+1 Morphological and Physiological Features Physical Characteristics: Bacillus circulans appears as rod-shaped bacteria measuring approximately 0.5-1.0 × 2.0-5.0 micrometers with rounded ends. The bacteria are motile via peritrichous flagella and form characteristic circular colonies with entire margins on standard growth media. semanticscholar Endospore Formation: Like other Bacillus species, Bacillus circulans produces highly resistant endospores that enable survival under extreme environmental conditions including heat, desiccation, radiation, and chemical stress. This spore-forming capability is crucial for maintaining viability during storage and application in agricultural systems. mdpi+1 Growth Requirements: The bacterium demonstrates remarkable adaptability to various pH levels (5.5-9.0), temperatures (15-45°C), and nutrient conditions. This physiological flexibility enables effective colonization across diverse soil types and climatic conditions. pmc.ncbi.nlm.nih+1 Metabolic Versatility and Enzyme Production Bacillus circulans produces an impressive array of extracellular enzymes that contribute to its agricultural and industrial significance: kasetsartjournal.ku+1 β-Mannanase Production: The bacterium produces thermostable β-mannanases used in biobleaching, coffee processing, animal feed improvement, and bioethanol production. These enzymes hydrolyze β-1,4-mannosidic linkages in mannan-based polysaccharides, generating valuable manno-oligosaccharides. kasetsartjournal.ku β-Galactosidase Activity: Bacillus circulans produces β-galactosidase enzymes used industrially for galactooligosaccharide (GOS) production from lactose. These prebiotic compounds have significant applications in food and pharmaceutical industries. pmc.ncbi.nlm.nih Chitinase and Cellulase Production: The organism secretes various polysaccharide-degrading enzymes including chitinases and cellulases, which contribute to organic matter decomposition and nutrient cycling in soil systems. mdpi+1 Additional Info Recommended Crops: Tomato, Banana, Rice Compatibility: Compatible with Bio Pesticides, Bio Fertilizers, and Plant growth hormones but not with chemical fertilizers and chemical pesticides. Shelf Life: Stable within 1 year from the date of manufacturing. Packing: We offer tailor-made packaging as per customers' requirements. Dosage & Application Wettable Powder: 1 x 10⁸ CFU per gram Foliar Application 1 Acre dose: 3-5 kg 1 Ha dose: 7.5 - 12.5 kg Soil Application (Soil drench or Drip irrigation) 1 Acre dose: 3-5 kg 1 Ha dose: 7.5 - 12.5 kg Soil Application (Soil drench or Drip irrigation) for Long duration crops / Orchards / Perennials 1 Acre dose: 3-5 kg 1 Ha dose: 7.5 - 12.5 kg Apply 2 times in 1 Year: Before onset of monsoon and after monsoon Seed Dressing 1 Kg seed: 10g Bacillus Circulans + 10g crude sugar Foliar Application for Long duration crops / Orchards / Perennials 1 Acre dose: 3-5 kg 1 Ha dose: 7.5 - 12.5 kg Apply 2 times in 1 Year: Before onset of monsoon and after monsoon Soluble Powder: 1 x 10⁹ CFU per gram Foliar Application 1 Acre dose: 1 kg 1 Ha dose: 2.5 kg Soil Application (Soil drench or Drip irrigation) 1 Acre dose: 1 kg 1 Ha dose: 2.5 kg Soil Application (Soil drench or Drip irrigation) for Long duration crops / Orchards / Perennials 1 Acre dose: 1 kg 1 Ha dose: 2.5 kg Apply 2 times in 1 Year: Before onset of monsoon and after monsoon Seed Dressing 1 Kg seed: 10g Bacillus Circulans + 10g crude sugar Foliar Application for Long duration crops / Orchards / Perennials 1 Acre dose: 1 kg 1 Ha dose: 2.5 kg Apply 2 times in 1 Year: Before onset of monsoon and after monsoon Seed Dressing Method Mix Bacillus Circulans with crude sugar in sufficient water to make a slurry. Coat seeds and dry in shade before sowing / broadcasting / dibbling in the field. Do not store treated / coated seeds for more than 24 hours. Soil Application Method: Mix at recommended doses with compost and apply at early life stages of crop along with other biofertilizers. Mix Bacillus Circulans at recommended doses in sufficient water and drench soil at early leaf stage / 2-4 leaf stage / early crop life cycle. Drip Irrigation: If there are insoluble particles, filter the solution and add it to the drip tank. For long duration crops / Perennial / Orchard crops: Dissolve Bacillus Circulans at recommended doses in sufficient water and apply as a drenching spray near the root zone twice a year. First application should be before the onset of the main monsoon / rainfall / spring season, and the second application after the main monsoon / rainfall / autumn / fall season. Foliar Application Method Apply foliar application at early disease incidence. Conduct 1-2 follow-up sprays at weekly intervals. Mix Bacillus Circulans at recommended doses in sufficient water and spray on foliage. Apply twice a year for long duration crops. First application should be before the onset of the main monsoon / rainfall / spring season, and the second application after the main monsoon / rainfall / autumn / fall season. Note: Do not store Bacillus Circulans solution for more than 24 hours after mixing in water. FAQ General Biology and Classification What is the current scientific name for Bacillus circulans? The organism is now properly classified as Niallia circulans (Jordan 1890) Gupta et al. 2020, following comprehensive phylogenetic analyses that led to taxonomic reclassification. However, it remains widely known in agricultural applications as Bacillus circulans. gbif+1 How does Bacillus circulans differ from Bacillus cereus? While both are spore-forming bacteria, Bacillus circulans (now Niallia circulans) is phylogenetically distinct from Bacillus cereus. Bacillus cereus belongs to the cereus group and can cause food poisoning, while Bacillus circulans is non-pathogenic and beneficial for plant growth. The two species differ significantly in their toxin production, metabolic capabilities, and safety profiles. wikipedia+4 Can Bacillus circulans cause infections in humans? Bacillus circulans is generally considered non-pathogenic to humans. Unlike Bacillus cereus, which can cause gastrointestinal illness, Bacillus circulans has no documented association with human disease when used in agricultural applications. However, as with any bacterial product, proper handling and application guidelines should be followed. mdpi Agricultural Applications and Effectiveness Which crops benefit most from Bacillus circulans application? Bacillus circulans is particularly effective for tomatoes, bananas, and rice, as specified in product recommendations. However, research shows benefits across diverse crops including cereals, vegetables, and fruits, especially in phosphorus-deficient soils or under stress conditions. mdpi+2 How quickly can farmers expect results from Bacillus circulans? Initial benefits typically become visible within 2-3 weeks as improved root development and enhanced nutrient uptake. Phosphate solubilization effects can be observed within days of application, while maximum growth promotion benefits develop over 6-8 weeks as bacterial populations establish in the rhizosphere. pubmed.ncbi.nlm.nih Can Bacillus circulans replace chemical fertilizers completely? While Bacillus circulans significantly enhances nutrient availability and can reduce fertilizer requirements by up to 25%, it works best as part of an integrated nutrient management system. Complete replacement of chemical fertilizers may be possible in organic systems with adequate organic matter and proper management practices. ojs.revistacontribuciones Safety and Compatibility Is Bacillus circulans safe for organic farming? Yes, Bacillus circulans is completely suitable for organic farming systems as it is a naturally occurring, non-GMO bacterium that enhances soil health and plant nutrition through biological processes. indogulfbioag+1 What should farmers avoid when using Bacillus circulans? Bacillus circulans is compatible with bio-pesticides, bio-fertilizers, and plant growth hormones but should not be applied simultaneously with chemical fertilizers or pesticides that may harm bacterial viability. Avoid storage of prepared solutions for more than 24 hours. indogulfbioag How should Bacillus circulans products be stored? Store products in cool, dry conditions away from direct sunlight and extreme temperatures. The spore-forming nature of Bacillus circulans allows products to maintain stability for up to one year from manufacture date when stored properly. indogulfbioag Technical and Application Questions What is the optimal application method for Bacillus circulans? Application methods include seed dressing (10g per kg seeds), soil application (3-5 kg per hectare), and foliar spray. For long-duration crops and orchards, apply twice yearly before and after monsoon seasons for optimal results. indogulfbioag Can Bacillus circulans be used in hydroponic systems? While traditionally used in soil-based systems, Bacillus circulans can potentially benefit hydroponic cultivation through its phosphate-solubilizing activity and plant hormone production, though specific formulations for soilless systems may require development. How does soil pH affect Bacillus circulans effectiveness? Bacillus circulans functions effectively across a wide pH range (5.5-9.0) but performs optimally in slightly acidic to neutral soils. Its acid-producing activity helps optimize soil conditions for nutrient availability. pmc.ncbi.nlm.nih Related Products Bacillus amyloliquefaciens Bacillus azotoformans Bacillus pumilus Pseudomonas fluorescens Pseudomonas putida Rhodococcus terrae Vesicular arbuscular mycorrhiza Williopsis saturnus More Products Resources Read all

Nulla facilisi. Integer commodo, augue ut feugiat faucibus, turpis risus ullamcorper erat, nec viverra sapien nunc in nulla. Posted on July 25, 2025 Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nulla facilisi. Integer commodo, augue ut feugiat faucibus, turpis risus ullamcorper erat, nec viverra sapien nunc in nulla. Quisque aliquet urna ut libero ultrices, non ullamcorper mi fermentum. Sed feugiat mauris ac felis elementum, ut tincidunt magna dapibus. Suspendisse commodo orci eget purus vulputate, eget ultricies turpis dictum. Vivamus dignissim, enim a sollicitudin scelerisque, velit mauris suscipit est, et viverra neque urna non lorem. Curabitur sit amet tristique lectus. Nam vitae quam et arcu volutpat tincidunt sed nec elit. Aenean porttitor leo at lectus dapibus, id luctus justo ornare. Sed convallis nisl sed sapien malesuada, eu dapibus ex bibendum. Curabitur luctus finibus tortor, id hendrerit ligula blandit eget. Phasellus ultricies turpis ut dolor ultrices, ut scelerisque arcu dignissim. Nulla tincidunt risus et justo tincidunt bibendum. Vestibulum sed urna tincidunt, pretium mauris ut, vehicula nisl. Nam gravida ullamcorper leo, nec malesuada lorem feugiat at. Etiam sed ex in est bibendum laoreet. Integer eu elit nec nunc suscipit pellentesque. Vestibulum in sagittis elit, sed volutpat nulla. Pellentesque rhoncus facilisis massa, ut tincidunt risus venenatis nec. Suspendisse tempor nulla turpis, sed elementum nunc gravida vel. Donec mattis ornare mauris, non laoreet ipsum euismod at. Vestibulum rhoncus dui sit amet magna fermentum, sit amet porttitor sem tincidunt. Sed nec nunc nec nibh lacinia fermentum. Nam volutpat, mi vel feugiat sagittis, felis ante viverra augue, a aliquet nibh turpis et libero. Nunc tincidunt metus vitae sem pretium, nec efficitur libero fermentum. Aliquam nec congue risus. Morbi feugiat quam in nibh cursus, eget tincidunt felis malesuada. Quisque a purus sed arcu laoreet efficitur. Cras interdum velit eu erat condimentum, et sagittis magna scelerisque. Suspendisse quis orci sit amet turpis lacinia consectetur. Cras varius quam sed lacus laoreet, ut elementum odio lacinia. Donec sed congue ligula, sed laoreet justo. Curabitur viverra nisi sit amet posuere fermentum. In et dui sed nunc cursus euismod et vitae metus. # # # About Indogulg BioAg Indogulf BioAg is the dedicated bio-technology division set-up under the Indogulf Group. We are pioneers in the development of biological inoculant, organic fertilizer and mycorrhiza (VAM). Our research & manufacturing facility is located in Salem, a small town in South India that is known for it’s rich underground water that promotes an extensive microbial population, making it an ideal hub for microbial bioscience. # # # Contact +1 437 774 3831 biosolutions@indogulfgroup.com What's New

Leading manufacturer & exporter of Hydromax nano fertilizer. Enhance crop yields with our advanced, eco-friendly solutions. Quality you can trust. < Nano Fertilizers Hydromax A comprehensive liquid nutrient solution containing essential elements such as N, P, K in ionic form, devoid of elements like chlorine found in mineral salts. Product Enquiry Download Brochure Benefits Promotes Strong and Healthy Plants Supports robust plant growth and development. Ionic Formulation Consists of basic elements like N, P, K in ionic form, free from other mineral salts. Flexible Nutrient Application Allows farmers to adjust nutrient application based on growth phases, species, and environmental factors. Recommended for Hydroponics Ideal for hydroponic systems due to its liquid form and high water solubility. Nutrient % (w/w) Form Nano nitrogen 4.01% Amino-chelated nitrate & ammonium 8 Conventional nitrogen 9.90% Stabilized nitrate 8 Nano phosphorus 0.87% Nano-sized phosphate 8 Nano potassium 0.93% Nano K⁺ ions 8 Nano calcium 0.06% Colloidal Ca²⁺ 8 Nano magnesium 0.04% Colloidal Mg²⁺ 8 Amino acids & enzymes 2% + QS Biostimulant package 8 Composition Dosage & Application Why choose this product Key Benefits Sustainability Advantage Additional Info FAQ Additional Info Compatibility: Compatible with chemical fertilizers and chemical pesticides Shelf life: Best before 24 months when stored at room temperature Packaging: 5 Ltx2/Corrugated Cardboard Box Why choose this product? One-Bottle Convenience: Replaces multiple A/B concentrates; no chlorine, no sodium 8 . Nano-Enhanced Uptake: Nano N, P, K, Ca and Mg ions enter root cells 3× faster than bulk salts 8 . pH-Stable Formula: Low buffering capacity keeps nutrient solution steady during recirculation. 24-Month Shelf Life: Remains crystal-clear; zero clogging in drip or NFT systems 8 . Key Benefits at a Glance Rapid Root Strike: Ionic N-P-K fuels immediate ATP synthesis for explosive root hairs. Balanced Growth Curve: Amino-chelated Mg and Ca keep photosynthesis and cell-wall growth in sync, preventing tip burn or blossom end-rot. Residue-Free Produce: Chlorine-free formulation stops salt spots on leafy greens. System Cleanliness: 100% water-soluble—no precipitates in emitters or reservoirs. Sustainability Advantage Hydromax consolidates multiple fertilizer SKUs into one recyclable container, cutting plastic waste by up to 70% and reducing on-farm storage space needs. Growers report 8–12% yield gains in leafy greens and a 15% faster crop cycle in hydroponic tomatoes. Implementation Checklist Replace existing page copy with the above sections, preserving current image URLs and schema markup. Add FAQ schema ( FAQPage JSON-LD) using the Q&A content to win Google rich snippets. Insert internal links to “Micromax,” “Nitromax” and the Indogulf blog articles on nutrient management to improve crawl depth. Compress brochure images to < 150 kB and add alt tags using target keywords (“phosphorus fertilizers,” “Hydromax nutrient solution”). Track organic keyword growth in Google Search Console; expect ranking improvements within 4–6 weeks for primary terms. Dosage & Application System Typical EC Target Hydromax Rate Notes NFT / Deep-water culture 1.2–1.8 mS/cm 5–7 mL/L stock tank Adjust based on crop phase Coco or soil drench 800–1,400 µS/cm 8–10 mL/L irrigation Flush every 3rd watering Foliar booster — 5 mL/L spray Use at early vegetative & pre-flower FAQ Is Hydromax only for hydroponics? No. Hydromax excels in hydroponics but also boosts yields in coco coir, peat mixes and field soils when applied via drip or foliar 8 . Do I still need calcium nitrate or Epsom salt? Generally no. Hydromax contains bioavailable nano Ca and Mg; however, heavy-fruiting crops may require a small Ca/Mg top-up late in the cycle. Will Hydromax clog my drip lines? The solution is fully ionic with < 1 µm particles, eliminating precipitation risk in emitters and foggers 8 . Can I mix Hydromax with pesticides or biostimulants? Yes. The formula is compatible with most biologicals and pesticides; always jar-test first if mixing oil-based products. What is the shelf life after opening? Store tightly sealed at 10–30 °C; product retains full potency for 24 months after manufacture 8 . Related Products Anpeekay NPK Nano Boron Nano Calcium Nano Chitosan Nano Copper Nano Iron Nano Potassium Nano Magnesium More Products Resources Read all

In agriculture Azospirillum lipoferum is used to promote root development and nitrogen fixation in various crops, leading to enhanced growth and higher agricultural productivity. < Microbial Species Azospirillum lipoferum In agriculture Azospirillum lipoferum is used to promote root development and nitrogen fixation in various crops, leading to enhanced growth and higher agricultural productivity. Strength 1 x 10⁸ CFU per gram / 1 x 10⁹ CFU per gram Product Enquiry Download Brochure Benefits Disease Suppression Suppresses soil-borne pathogens through competition for nutrients and production of antimicrobial compounds, enhancing plant health and reducing disease incidence. Plant Growth Promotion Produces plant growth-promoting substances like auxins and cytokinins, stimulating root growth and overall plant development. Phosphate Solubilization Releases phosphate bound in the soil, making it available for plant uptake, thereby improving phosphorus nutrition. Nitrogen Fixation Converts atmospheric nitrogen into ammonia, enhancing soil fertility and reducing the need for nitrogen fertilizers. Dosage & Application Additional Info Scientific References Mode of Action FAQ Scientific References 1. Azospirillum, a free-living nitrogen-fixing bacterium closely associated with grasses: genetic, biochemical and ecological aspects URL: https://academic.oup.com/femsre/article/24/4/487/510690 Journal : FEMS Microbiology Reviews (2000) 2. Azospirillum: benefits that go far beyond biological nitrogen fixation URL: https://pmc.ncbi.nlm.nih.gov/articles/PMC5935603/ Journal : PMC - PubMed Central (2018) 3. Field-based assessment of the mechanism of maize yield enhancement by Azospirillum lipoferum CRT1 URL: https://www.nature.com/articles/s41598-017-07929-8 Journal : Scientific Reports - Nature (2017) 4. Posttranslational regulation of nitrogenase activity in Azospirillum brasilense URL: https://pmc.ncbi.nlm.nih.gov/articles/PMC196782/ Journal : Journal of Bacteriology 5. Molecular Mechanisms Determining the Role of Bacteria from the Genus Azospirillum in Plant Growth Promotion URL: https://pmc.ncbi.nlm.nih.gov/articles/PMC10252715/ Journal : International Journal of Molecular Sciences (2023) Mode of Action Biological Nitrogen Fixation Mechanism Azospirillum lipoferum converts atmospheric nitrogen (N₂) into ammonium (NH₄⁺) under microaerobic conditions through the nitrogenase enzyme complex . This complex consists of two essential components: the dinitrogenase protein (MoFe protein, NifDK) containing a molybdenum-iron cofactor where N₂ reduction occurs, and the dinitrogenase reductase protein (Fe protein, NifH) that transfers electrons to the nitrogenase protein. The efficiency of nitrogen fixation in A. lipoferum reaches 48 mg total nitrogen per gram glucose consumed at late log phase, with approximately 25% of fixed nitrogen recovered in culture supernatants . This bacterium demonstrates a unique hydrogenase system - an active uptake hydrogenase that increases during glucose limitation and serves as an oxygen protection mechanism for the oxygen-sensitive nitrogenase. Molecular Regulation Systems Transcriptional Control The nitrogen fixation process is regulated by multiple nif genes including the nifHDK operon encoding nitrogenase components and nifA as the transcriptional activator. Expression is controlled by the general nitrogen regulatory system involving NtrBC proteins and the alternative sigma factor σ⁵⁴ (RpoN). Post-translational Regulation A. lipoferum employs reversible ADP-ribosylation of the nitrogenase iron protein mediated by DraT (ADP-ribosyltransferase) and DraG (activating glycohydrolase) enzymes. This mechanism provides rapid response to environmental changes - nitrogenase becomes inactive when ADP-ribosylated in the presence of ammonium or anaerobic conditions, and reactivated when ADP-ribosyl groups are removed. Plant Growth Promotion Mechanisms Phytohormone Production A. lipoferum synthesizes multiple plant hormones through distinct biosynthetic pathways. The bacterium produces indole-3-acetic acid (IAA) via the indole-3-pyruvate (IPyA) pathway using the key enzyme indole-3-pyruvate decarboxylase encoded by ipdC . It also demonstrates capacity for gibberellin metabolism , effectively hydrolyzing GA₂₀-glucosyl conjugates and performing 3β-hydroxylation to convert GA₂₀ to the bioactive GA₁. The bacterium expresses ACC deaminase which hydrolyzes the ethylene precursor 1-aminocyclopropane-1-carboxylate , reducing plant ethylene levels and promoting growth. Additional hormones include cytokinins through octaprenyl diphosphate synthase activity. Root System Architecture Modification Inoculation with A. lipoferum results in altered root morphology characterized by increased lateral root formation and enhanced root hair development. This root system expansion allows plants to explore larger soil volumes for nutrient and water acquisition. Field studies demonstrate that these morphological changes occur early in plant development and correlate with improved photosynthetic potential and reduced glucose content in ascending sap. Root Colonization and Plant Interaction Attachment Mechanisms A. lipoferum employs a two-step colonization process . Initial adsorption is mediated by the polar flagellum , whose flagellin protein is a glycoprotein essential for motility-dependent attachment. The subsequent anchoring phase involves unidentified surface polysaccharides that facilitate stable root surface colonization. Energy taxis plays a crucial role in root colonization, with bacteria navigating toward metabolizable compounds in root exudates. This chemotactic response contributes to the broad host range observed in Azospirillum -plant associations. Mineral Nutrition Enhancement A. lipoferum demonstrates phosphate solubilization ability , though weaker than specialized phosphate-solubilizing bacteria. The mechanism involves organic acid production (primarily acetic acid) that reduces medium pH and releases soluble phosphate from calcium phosphate complexes . Coimmobilization with other phosphate-solubilizing bacteria like Bacillus megaterium significantly enhances phosphate availability. The bacterium also participates in iron nutrition through potential siderophore production and iron chelation mechanisms , though specific iron acquisition systems require further characterization. Agricultural Applications and Field Performance Commercial Inoculant Effectiveness Field studies with commercial strain A. lipoferum CRT1 demonstrate variable but significant yield enhancement across different agricultural sites. The bacterium's effectiveness depends on soil characteristics and environmental conditions , with survival on maize roots limited to approximately 57 days post-inoculation . Research indicates that A. lipoferum inoculation can substitute for 50% of nitrogen fertilizer applications without yield reduction , demonstrating potential for sustainable agriculture practices. The bacterium shows particular efficacy when applied as seed coating formulations. Stress Tolerance Mechanisms A. lipoferum confers drought tolerance through multiple mechanisms including abscisic acid synthesis , osmotic adjustment , and antioxidant enzyme activation . The bacterium induces expression of stress-related genes and enhances water use efficiency in treated plants. Additional Info Recommended Crops: Cereals, Millets, Pulses, Oilseeds, Fibre Crops, Sugar Crops, Forage Crops, Plantation crops, Vegetables, Fruits, Spices, Flowers, Medicinal crops, Aromatic Crops, Orchards, and Ornamentals. Compatibility: Compatible with Bio Pesticides, Bio Fertilizers, and Plant growth hormones but not with chemical fertilizers and chemical pesticides. Shelf Life: Stable within 1 year from the date of manufacturing. Packing: We offer tailor-made packaging as per customers' requirements. Dosage & Application Seed Coating/Seed Treatment: Coat 1 kg of seeds with a slurry mixture of 10 g of Azospirillum Lipoferum and 10 g of crude sugar in sufficient water. Dry the coated seeds in shade before sowing or broadcasting in the field. Seedling Treatment: Dip seedlings into a mixture of 100 grams of Azospirillum Lipoferum with sufficient water. Soil Treatment: Mix 3-5 kg per acre of Azospirillum Lipoferum with organic manure or fertilizers. Incorporate into the soil during planting or sowing. Irrigation: Mix 3 kg per acre of Azospirillum Lipoferum in water and apply through drip lines. FAQ What physiological mechanisms underlie the benefits of Azospirillum lipoferum in crops? Azospirillum lipoferum is a diazotrophic bacterium that colonizes the rhizosphere and endorhizally associates with plant roots. Through biological nitrogen fixation via the nitrogenase enzyme complex, it converts atmospheric N₂ into bioavailable ammonia, enhancing plant nitrogen nutrition. Additionally, it synthesizes phytohormones (indole-3-acetic acid, gibberellins) that modulate root architecture—promoting lateral root proliferation and root hair elongation—thereby increasing absorptive surface area and nutrient uptake efficiency. How is Azospirillum lipoferum formulated and applied in agronomic practice? Seed Inoculation: Prepare a peat-based carrier formulation containing ≥10⁸ CFU/g. Coat seeds at 10 g inoculant per kg seed, ensuring uniform adhesion with an adhesive such as sterile sucrose solution. Air-dry for 30–60 minutes prior to sowing. Seedling Root Dip: Suspend 100 g of inoculum in 10 L of sterile water and dip root systems of nursery seedlings for 15 minutes before transplanting. Soil Amendment: Incorporate 3–5 kg inoculant per hectare into the top 10 cm of soil, preferably mixed with well-decomposed organic manure. Liquid Delivery: Dissolve 3 kg inoculant in 1,000 L of irrigation water and apply via drip or furrow irrigation systems to distribute cells throughout the rhizosphere. Which agronomic crops demonstrate optimal responsiveness to Azospirillum lipoferum inoculation? Field and greenhouse trials indicate significant yield and biomass improvements in Poaceae (wheat, maize, rice, sorghum), Fabaceae (pulses), Brassicaceae (oilseeds), Solanaceae (tomato, pepper), and Cucurbitaceae (cucumber, melon). Enhanced root development and N-use efficiency have been documented across cereals, legumes, oilseeds, horticultural, and fiber crops. What compatibility and biosafety considerations apply to Azospirillum lipoferum applications? Azospirillum lipoferum formulations are biosafe, exhibiting no pathogenicity to plants, humans, or animals. The bacterium is compatible with organic amendments, biofertilizers, and select biopesticides. Physical or chemical incompatibilities may arise when co-applied with high concentrations of synthetic fertilizers or broad-spectrum biocides; sequential rather than simultaneous application is recommended to maintain cell viability. What are the recommended storage conditions and shelf life parameters for Azospirillum lipoferum inoculants? Maintain formulations at 4–10 °C in moisture-proof, opaque packaging. Under these conditions, viable cell counts remain ≥10⁷ CFU/g for 9–12 months post-manufacture. Prolonged exposure to temperatures above 25 °C or high relative humidity reduces survival rates and inoculum efficacy. By what mechanisms does Azospirillum brasilense enhance plant growth and stress tolerance? Azospirillum brasilense is a facultative endophyte that fixates atmospheric nitrogen via nitrogenase activity and secretes a suite of phytohormones (auxins, cytokinins, gibberellins). It also produces exopolysaccharides that improve soil aggregation and water retention. Through phosphorus solubilization (organic acid secretion) and induced systemic tolerance—mediated by modulation of stress-responsive gene expression—A. brasilense ameliorates abiotic stresses such as drought and salinity. What application methodologies are employed for Azospirillum brasilense in crop production? Seed Coating: Utilize a carrier-based formulation (≥10⁸ CFU/g) at 10 g per kg of seed, combined with a polymeric sticker to ensure uniform adhesion. Soil Application: Distribute 3–5 kg inoculant per hectare by broadcasting or banding, integrating with organic fertilizer or compost. Irrigation Integration: Infuse 3 kg inoculant into 1,000 L irrigation solution and apply through drip or sprinkler systems to achieve homogeneous microbial delivery. Which crop species exhibit pronounced yield responses to Azospirillum brasilense? Empirical studies demonstrate yield enhancements in cereals (wheat, maize, rice), legumes (soybean, chickpea), oilseeds (canola, sunflower), and various vegetables (tomato, eggplant) when inoculated with A. brasilense under both irrigated and rainfed conditions. How does Azospirillum brasilense interact at the molecular level with host plants? Upon root colonization, A. brasilense secretes signaling molecules—N-acyl homoserine lactones and lipo-chitin oligosaccharides—that trigger root gene networks involved in nutrient transport and stress responses. The bacterium’s nitrogenase complex reduces N₂, while secreted indole-3-acetic acid influences auxin-responsive transcription factors, collectively fostering root proliferation and enhanced nutrient assimilation. Are there any safety or environmental risks associated with Azospirillum brasilense use? A. brasilense poses negligible biosafety risks; it is non-pathogenic to non-target organisms and does not persist as a pollutant in soil ecosystems. Compatibility with most agrochemicals is high, though cell viability may decrease in the presence of potent oxidizing agents or extreme pH conditions. Related Products Acetobacter xylinum Azospirillum brasilense Azospirillum spp. Azotobacter vinelandii Beijerinckia indica Bradyrhizobium elkanii Bradyrhizobium japonicum Gluconacetobacter diazotrophicus More Products Resources Read all

Rhodospirillum molischianum is a phototrophic bacterium known for its role in anoxygenic photosynthesis. It uses bacteriochlorophylls to capture light energy and metabolize organic compounds, thriving in low-oxygen aquatic and soil environments. This bacterium contributes to carbon cycling and plays a role in ecosystem nutrient balance. Its well-studied light-harvesting complexes make it a model organism for research into photosynthetic mechanisms and bioenergetics, with potential applications in bioenergy and environmental management. < Microbial Species Rhodospirillum molischianum Rhodospirillum molischianum is a phototrophic bacterium known for its role in anoxygenic photosynthesis. It uses bacteriochlorophylls to capture light energy and metabolize organic compounds, thriving… Show More Strength 1 x 10⁹ CFU per gram / 1 x 10¹⁰ CFU per gram Product Enquiry Download Brochure Benefits Hydrogen Production Capable of producing hydrogen gas, offering potential for renewable energy solutions. Soil Health Enhancement Improves nutrient cycling in soils, promoting plant growth and ecosystem vitality. Photosynthetic Growth Utilizes light energy for metabolic processes, supporting sustainable biomass production. Organic Pollutant Biodegradation Effectively degrades organic contaminants, contributing to bioremediation efforts. Dosage & Application Additional Info Scientific References Mode of Action FAQ Scientific References Content coming soon! Mode of Action Content coming soon! Additional Info Contact us for more details Dosage & Application Contact us for more details FAQ Content coming soon! Related Products Saccharomyces cerevisiae Bacillus polymyxa Thiobacillus novellus Thiobacillus thiooxidans Alcaligenes denitrificans Bacillus licheniformis Bacillus macerans Citrobacter braakii More Products Resources Read all

< Animal Health Feed Pro Feed Pro (Microbial feed additive for calves) enhances greater feed intake of Product Enquiry Benefits Reduces Disease Risk and Strengthens Gut Integrity Lowers the incidence of diarrheal diseases and intestinal infections, while bolstering gut integrity and improving overall health status. Improves Stress Adaptation and Growth Rate Helps animals adapt to stress quickly, increases average daily gain, and shortens the time to market, reducing overall input costs. Enhances Feed Intake and Conversion Efficiency Improves appetite and feed conversion, resulting in better growth and more efficient nutrient use. Supports Digestion and Gut Health Promotes healthy digestion, strengthens gastrointestinal function, and contributes to better calf performance and immune development. Component Amount per kg Bioactive Chromium 65 mg Calcium 240 g Phosphorus 120 g Magnesium 2.11 g Zinc 2.13 g Copper 312 mg Cobalt 45 mg Iron 1000 mg Iodine 160 mg DL-Methionine 2.00 g L-Lysine 4.00 g Protein Hydrolysate 4.00 g Composition Dosage & Application Additional Info Dosage & Application Content coming soon! Additional Info Content coming soon! Related Products Stress Pro Camel Care Pro Cattle Care Max Cattle Care Pro Grass Mask Lactomine Pro Lactomix Mineral Max Pastocare Calf Pro More Products Resources Read all

Lactobacillus bulgaricus aids in lactose digestion, promotes gut health, and is commonly used in yogurt production for probiotic benefits. < Microbial Species Lactobacillus bulgaricus Lactobacillus bulgaricus aids in lactose digestion, promotes gut health, and is commonly used in yogurt production for probiotic benefits. Strength 1 x 10⁸ CFU per gram / 1 x 10⁹ CFU per gram Product Enquiry Download Brochure Benefits Yogurt Fermentation This probiotic is essential for yogurt production, contributing to its unique flavor, texture, and health benefits. Nutrient Absorption Improvement It aids in the absorption of essential nutrients, such as vitamins and minerals, promoting overall health. Digestive Health Enhancement It supports digestive health by improving gut microbiota balance and alleviating symptoms of lactose intolerance. Immune System Support This strain boosts immune function by stimulating the production of immune cells and enhancing the body’s defense against pathogens. Dosage & Application Additional Info Scientific References Mode of Action FAQ Scientific References Content coming soon! Mode of Action Content coming soon! Additional Info Key Features All microbial strains are characterized using 16S rDNA. All products are non-GMO. No animal-derived materials are used. The typical shelf life is 2 years. All strains are screened in-house using high-throughput screening methods. We can customize manufacturing based on the required strength and dosage. High-resilience strains Stable under a wide pH range Stable under a broad temperature range Stable in the presence of bile salts and acids Do not show antibiotic resistance Packaging Material The product is packaged in a multi-layer, ultra-high barrier foil that is heat-sealed and placed inside a cardboard shipper or plastic drum. Shipping Shipping is available worldwide. Probiotic packages are typically transported in insulated Styrofoam shippers with dry ice to avoid exposure to extreme high temperatures during transit. Support Documentation Certificate of Analysis (COA) Specifications Material Safety Data Sheets (MSDS) Stability studies (18 months) Certifications ISO 9001 ISO 22000 HACCP Halal and Kosher Certification (for Lactobacillus strains) FSSAI Dosage & Application Contact us for more details FAQ Content coming soon! Related Products Bifidobacterium animalis Bifidobacterium bifidum Bifidobacterium breve Bifidobacterium infantis Bifidobacterium longum Clostridium butyricum Lactobacillus acidophilus Lactobacillus casei More Products Resources Read all

Paracoccus denitrificans is a beneficial bacterium known for its nitrate-reducing properties, specifically its ability to convert nitrate to nitrogen gas. < Microbial Species Paracoccus denitrificans Paracoccus denitrificans is a beneficial bacterium known for its nitrate-reducing properties, specifically its ability to convert nitrate to nitrogen gas. Strength 1 x 10⁸ CFU per gram / 1 x 10⁹ CFU per gram Product Enquiry Download Brochure Benefits Treatment Efficiency Returns alkalinity to the treatment process, supporting efficient wastewater treatment. Groundwater Protection Prevents groundwater pollution by reducing nitrate levels from agricultural or residential fertilizers. Nitrogen Management Reduces inorganic nitrogen to nitrous oxide, aiding in environmental nitrogen management. Water Quality Improvement Removes nitrogen from sewage and municipal wastewater, improving water quality. Dosage & Application Additional Info Scientific References Mode of Action FAQ Scientific References Olaya-Abril, A., Luque-Almagro, V. M., Manso, I., Moreno-Vivián, C., & Roldán, M. D. (2018). Exploring the Denitrification Proteome of Paracoccus denitrificans PD1222. Frontiers in Microbiology , 9, 1137. DOI: 10.3389/fmicb.2018.01137 Bordel, S., Rodríguez, Y., Muñoz, R., & Lebrero, R. (2024). Genome-scale metabolic model of the versatile bacterium Paracoccus denitrificans Pd1222. mSystems , 9(1), e01077-23. DOI: 10.1128/msystems.01077-23 Hahnke, S. M., Moosmann, P., Erb, T. J., & Strous, M. (2014). An improved medium for the anaerobic growth of Paracoccus denitrificans Pd1222. Frontiers in Microbiology , 5, 18. DOI: 10.3389/fmicb.2014.00018 Kumar, S., Ridge, J. P., Arce-Rodriguez, A., Jeuken, L. J. C., Richardson, D. J., & Hough, M. A. (2017). Environmental and Genetic Determinants of Biofilm Formation in Paracoccus denitrificans. Applied and Environmental Microbiology , 83(18), e01350-17. DOI: 10.1128/AEM.01350-17 Olaya-Abril, A., Hidalgo-Carrillo, J., Luque-Almagro, V. M., Fuentes-Almagro, C., Moreno-Vivián, C., Richardson, D. J., & Roldán, M. D. (2021). Effect of pH on the denitrification proteome of the soil bacterium Paracoccus denitrificans. Scientific Reports , 11, 17261. DOI: 10.1038/s41598-021-96559-2 Baumann, B., Snozzi, M., Zehnder, A. J., & van der Meer, J. R. (1996). Dynamics of denitrification activity of Paracoccus denitrificans during changes from aerobic to anaerobic growth conditions and vice versa. Journal of Bacteriology , 178(16), 4678-4687. Giannopoulos, G., Sullivan, M. J., Hartop, K. R., Rowley, G., Gates, A. J., Watmough, N. J., & Richardson, D. J. (2017). Tuning the modular Paracoccus denitrificans respirome to adapt from aerobic respiration to anaerobic denitrification. Environmental Microbiology , 19(12), 4953-4964. Jarman, O. D., Biner, O., Hirst, J., & Sazanov, L. A. (2021). Paracoccus denitrificans: a genetically tractable model system for studying respiratory complex I. Scientific Reports , 11, 10143. DOI: 10.1038/s41598-021-89575-9 Mode of Action Paracoccus denitrificans operates through a sophisticated four-step denitrification pathway that makes it highly valuable for agricultural and environmental applications : pmc.ncbi.nlm.nih+2 Sequential Reduction Process Nitrate → Nitrite: Via nitrate reductase (NAR/NAP) Nitrite → Nitric Oxide: Through cytochrome cd₁ nitrite reductase Nitric Oxide → Nitrous Oxide: Using nitric oxide reductase (NOR) Nitrous Oxide → Nitrogen Gas: Final step via nitrous oxide reductase (NosZ) This complete pathway effectively removes excess nitrogen from soil and water systems, preventing environmental pollution and supporting sustainable agriculture. wikipedia+1 Paracoccus denitrificans demonstrates multiple sophisticated biochemical mechanisms that make it a valuable bacterial species for agricultural and environmental applications: Denitrification Pathway The primary mode of action involves a sequential four-step reduction process under anaerobic conditions: Nitrate Reduction: Membrane-bound nitrate reductase (NAR) and periplasmic nitrate reductase (NAP) convert nitrate (NO₃⁻) to nitrite (NO₂⁻) Nitrite Reduction: Cytochrome cd₁ nitrite reductase (NIR) reduces nitrite to nitric oxide (NO) Nitric Oxide Reduction: Nitric oxide reductase (NOR) converts NO to nitrous oxide (N₂O) Nitrous oxide Reduction: Nitrous oxide reductase (NosZ) completes the pathway by reducing N₂O to nitrogen gas (N₂) This complete denitrification pathway effectively removes excess nitrogen from soil and water systems, preventing environmental pollution and eutrophication. Metabolic Versatility P. denitrificans exhibits remarkable metabolic flexibility: Facultative anaerobe: Can switch between aerobic respiration and anaerobic denitrification Chemolithoautotrophic capabilities: Can utilize various carbon sources including C1 compounds (methanol, formate) Energy conservation: Couples denitrification to ATP synthesis through respiratory chain Enzyme Regulation The bacterium employs sophisticated regulatory mechanisms: FnrP transcription factor: Responds to oxygen levels, activating denitrification genes under anoxic conditions NarR, NirI, and NosR regulators: Specifically control expression of nitrate, nitrite, and nitrous oxide reductase genes Trace element dependency: Requires iron, molybdenum, copper, and zinc for optimal enzyme function Additional Info Shelf Life: Stable within 1 year from the date of manufacturing. Packing: We offer tailor-made packaging as per customers' requirements. Dosage & Application Contact us for more details FAQ Is Paracoccus denitrificans pathogenic? No, Paracoccus denitrificans is completely non-pathogenic to humans, animals, and plants. Research confirms it's classified as a beneficial environmental bacterium with no known health risks. Unlike pathogenic bacteria, it's widely used safely in agricultural applications and bioaugmentation programs. aquaculturesciencemanagement.biomedcentral+1 Key Differences: Paracoccus vs Pseudomonas denitrificans These are distinct bacterial species with different applications : wikipedia+1 Paracoccus denitrificans Alpha-proteobacteria, spherical morphology Environmental nitrogen cycling and soil health Complete denitrification capabilities Agricultural and wastewater treatment applications Pseudomonas denitrificans Gamma-proteobacteria, rod-shaped Industrial vitamin B12 production (up to 198+ mg/L) Pharmaceutical manufacturing Biotechnological applications Environmental Habitat and Distribution Paracoccus denitrificans is ubiquitously distributed across multiple environments : pmc.ncbi.nlm.nih+2 Soil ecosystems: Primary habitat in agricultural and forest soils Wastewater treatment facilities: Naturally occurring in activated sludge Marine environments: Sediments and water columns Plant rhizosphere: Root zone associations Biofilm communities: Surface-attached growth in aquatic systems Industrial Biotechnology Applications Both species have significant biotechnology potential: journals.asm+2 Paracoccus denitrificans Promising cell factory for metabolic engineering Genetic tractability for strain development Bioremediation applications Wastewater treatment systems Pseudomonas denitrificans Major industrial vitamin B12 producer 120,000L fermenter operations Pharmaceutical compound manufacturing Enzyme production systems Environmental Importance Denitrifying bacteria provide critical ecosystem services : indogulfbioag+2 Nitrogen Cycle Regulation Convert excess nitrates back to atmospheric nitrogen Prevent nitrogen accumulation disrupting ecosystems Control nitrogen availability in terrestrial and aquatic systems Pollution Control Remove agricultural nitrate pollution from groundwater Prevent eutrophication and harmful algal blooms Protect water quality and drinking water safety Climate Impact Minimize N₂O greenhouse gas emissions when properly managed Support methane oxidation in aquatic systems Contribute to soil carbon sequestration Is Paracoccus denitrificans pathogenic? No, Paracoccus denitrificans is not pathogenic to humans or plants. It is classified as a non-pathogenic, beneficial environmental bacterium. Research has confirmed that P. denitrificans poses no known health risks and is widely used in agricultural and environmental applications. Unlike some bacterial species that can cause disease, P. denitrificans is considered safe for use in bioaugmentation and soil improvement programs. What is the meaning of Pseudomonas denitrificans? Pseudomonas denitrificans refers to a different bacterial species (now classified as part of the P. pertucinogena group) that was first isolated from garden soil in Vienna, Austria. The name "Pseudomonas" means "false unit" in Greek, while "denitrificans" indicates its ability to perform denitrification. This gram-negative aerobic bacterium is primarily known for its vitamin B12 overproduction capabilities and has been used industrially for cobalamin (vitamin B12) manufacturing. What is the function of Pseudomonas denitrificans? Pseudomonas denitrificans serves several important functions: Vitamin B12 Production: Primary industrial use for manufacturing cobalamin through fermentation Denitrification: Converts nitrate to nitrogen gas under anaerobic conditions Methionine Synthesis: Uses vitamin B12 for amino acid metabolism Biotechnological Applications: Engineered strains are used in pharmaceutical production Environmental Role: Participates in nitrogen cycling in soil ecosystems Where is Paracoccus denitrificans commonly found? Paracoccus denitrificans is ubiquitously distributed in various environments: Soil ecosystems: Most common habitat, particularly in agricultural soils Sewage treatment plants: Naturally occurring in activated sludge systems Marine environments: Found in sediments and water columns Plant rhizosphere: Associates with root zones of various crops Wastewater systems: Thrives in nitrogen-rich industrial effluents Biofilms: Forms surface-attached communities in aquatic environments The bacterium shows particular preference for environments with fluctuating oxygen conditions, making it well-adapted to dynamic agricultural and treatment system environments. How does Paracoccus denitrificans help in the nitrogen cycle? Paracoccus denitrificans plays a critical role as a nitrogen cycle closer through complete denitrification: Primary Functions Nitrate Removal: Converts excess soil nitrates (NO₃⁻) to harmless nitrogen gas (N₂) Pollution Prevention: Prevents nitrate leaching into groundwater and surface water bodies Atmospheric Return: Returns fixed nitrogen back to the atmospheric reservoir Eutrophication Control: Reduces nutrient loads that cause algal blooms in water bodies Environmental Benefits Soil Health: Prevents nitrate accumulation that can harm soil microbiology Water Quality: Reduces nitrogen pollution in aquatic ecosystems Greenhouse Gas Mitigation: Properly managed denitrification minimizes N₂O emissions Sustainable Agriculture: Provides biological nitrogen management solution Is Pseudomonas denitrificans used in vitamin B12 production? Yes, Pseudomonas denitrificans is extensively used for industrial vitamin B12 production. It is one of only two major bacterial strains (alongside Propionibacterium shermanii) used commercially for cobalamin manufacturing. Key aspects include: Production Capabilities High Yield: Can produce up to 198+ mg/L of vitamin B12 in large-scale fermenters Aerobic Process: Produces B12 under oxygen-rich conditions (unlike most B12 producers) Cost-Effective: Uses inexpensive substrates like maltose syrup and corn steep liquor Genetic Engineering: Strains with overexpressed cob genes achieve enhanced production Commercial Applications Pharmaceutical Industry: Primary source for B12 supplements and medications Food Industry: Used in fortified foods and animal feed additives Biotechnology: Research model for cobalamin biosynthesis studies What role does Paracoccus denitrificans play in denitrification? Paracoccus denitrificans is considered a model organism for complete denitrification due to its exceptional capabilities: Complete Pathway Execution All Four Steps: Performs the entire nitrate → nitrite → nitric oxide → nitrous oxide → nitrogen gas sequence High Efficiency: Achieves rapid and complete nitrogen oxide reduction Minimal Intermediates: Produces primarily N₂ with minimal accumulation of harmful intermediates like N₂O Unique Characteristics Respiratory Coupling: Links denitrification to energy generation for growth Oxygen Tolerance: Can perform "aerobic denitrification" under low oxygen conditions Metabolic Flexibility: Uses various organic substrates as electron donors Environmental Adaptation: Functions effectively across wide pH and temperature ranges Applications Wastewater Treatment: Used in biological nutrient removal systems Bioremediation: Cleans up nitrogen-contaminated environments Agricultural Systems: Natural soil denitrification processes Can Pseudomonas denitrificans be used in industrial biotechnology? Yes, Pseudomonas denitrificans has significant industrial biotechnology applications: Current Industrial Uses Vitamin B12 Manufacturing: Primary commercial application in 120,000L fermenters Pharmaceutical Production: Source of therapeutic cobalamin compounds Enzyme Production: Produces industrially relevant dehydrogenases and reductases Metabolic Engineering Platform: Chassis for producing various biochemicals Biotechnology Potential Genetic Tractability: Amenable to genetic modifications and strain improvement Process Optimization: Responds well to fermentation parameter control Substrate Flexibility: Can utilize various carbon sources including waste materials Scale-Up Capability: Successfully operates at industrial production scales Research Applications Cobalamin Pathway Studies: Model for understanding B12 biosynthesis Metabolic Engineering: Platform for producing novel compounds Systems Biology: Well-characterized genome and proteome for systems-level studies How are Paracoccus and Pseudomonas denitrificans different? Paracoccus denitrificans and Pseudomonas denitrificans are distinct bacterial species with different taxonomic classifications and primary functions: Taxonomic Differences Paracoccus denitrificans: Alpha-proteobacteria, spherical (coccoid) morphology Pseudomonas denitrificans: Gamma-proteobacteria, rod-shaped morphology Primary Functions Paracoccus: Environmental nitrogen cycling, complete denitrification, bioremediation Pseudomonas: Industrial vitamin B12 production, pharmaceutical manufacturing Metabolic Characteristics Paracoccus: Versatile carbon source utilization, biofilm formation, soil adaptation Pseudomonas: Specialized cobalamin overproduction, aerobic B12 synthesis Applications Paracoccus: Agriculture, wastewater treatment, environmental remediation Pseudomonas: Pharmaceutical industry, biotechnology, vitamin manufacturing Environmental Roles Paracoccus: Soil health, nitrogen pollution control, ecosystem balance Pseudomonas: Limited environmental role, primarily industrial applications Why are denitrifying bacteria like Paracoccus and Pseudomonas important in the environment? Denitrifying bacteria are essential environmental regulators that provide critical ecosystem services: Nitrogen Cycle Completion Atmospheric Return: Convert excess soil nitrates back to atmospheric nitrogen Cycle Balance: Prevent nitrogen accumulation that would disrupt ecosystem balance Natural Regulation: Control nitrogen availability in terrestrial and aquatic systems Pollution Control Nitrate Remediation: Remove agricultural nitrate pollution from groundwater Eutrophication Prevention: Reduce nitrogen loads causing harmful algal blooms Water Quality Protection: Maintain safe drinking water by controlling nitrate levels Climate Impact Greenhouse Gas Regulation: When properly managed, minimize N₂O emissions Methane Oxidation: Some denitrifiers also consume methane in aquatic systems Carbon Sequestration: Support soil organic matter accumulation through ecosystem health Agricultural Sustainability Soil Health Maintenance: Prevent toxic nitrate buildup in agricultural soils Natural Fertility Cycling: Support sustainable nitrogen management systems Biological Remediation: Provide natural solutions for nitrogen-contaminated sites Ecosystem Services Biodiversity Support: Maintain balanced nutrient cycles supporting diverse communities Habitat Protection: Preserve aquatic and terrestrial ecosystem integrity Resource Conservation: Reduce need for expensive chemical remediation technologies Related Products More Products Resources Read all