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Enhance flowering with Bloom Up by Indogulf BioAg. Premium, organic plant protection for vibrant blooms. Trusted manufacturer and exporter of quality solutions. < Plant Protect Bloom Up Reflective anti-transpirant from vegetable oil that enhances drought tolerance, reduces water loss, and improves crop health. Non-toxic and biodegradable. Product Enquiry Download Brochure Benefits Improves Post-Harvest Quality Improves the post-harvest keeping quality and vase life of crops by maintaining cell turgidity and overall freshness. Reduces Water Loss Effectively reduces water loss from the plant surface through reflecting a greater amount of incident light than normal. Maintains Turgor Pressure Helps to maintain the relative water content and turgor of the plant, ensuring cell turgidity even after harvest. Enhances Stress Resistance Helps plants recover from thermic and cold stress, improving resistance to drought. Composition It is based on long chain fatty alcohol derived from non-edible vegetable oil. Composition Dosage & Application Key Benefits FAQ Additional Info Additional Info Mode of Action BLOOM UP primarily functions by reflecting the sun’s rays. When applied as a foliar spray, it creates a thin glassy film-coat that enhances light reflection, thereby reducing the thermal impact on plant tissues. Recommended Crops Cereals, Millets, Pulses, Oilseeds, Fibre Crops, Sugar Crops, Forage Crops, Plantation Crops, Vegetables, Fruits, Spices, Flowers, Medicinal Crops, Aromatic Crops, Orchards, and Ornamentals. Shelf Life & Packaging Storage: Store in a cool, dry place at room temperature Shelf Life: 24 months from the date of manufacture at room temperature Packaging: 1 litre / 5 litre FAQ What is Bloom Up used for? Bloom Up is used as a natural anti-transpirant and anti-stress plant feed. It helps reduce water loss from plant surfaces, improves drought tolerance, supports crop health, and helps maintain post-harvest freshness. How does Bloom Up help plants during drought stress? Bloom Up creates a thin reflective film on plant surfaces. This helps reflect part of the sun’s rays, reduces thermal stress on plant tissues, and helps limit excessive moisture loss during drought, heat, or water-stress conditions. Can Bloom Up help improve post-harvest quality? Yes. Bloom Up helps maintain cell turgidity and freshness by reducing water loss. This can support better post-harvest keeping quality, firmness, and vase life in suitable crops. Which crops can Bloom Up be used on? Bloom Up can be used on a wide range of crops, including cereals, millets, pulses, oilseeds, fibre crops, sugar crops, forage crops, plantation crops, vegetables, fruits, spices, flowers, medicinal crops, aromatic crops, orchards, and ornamentals. How should Bloom Up be applied? Mix 3–5 ml of Bloom Up per 1 litre of water . Apply as a foliar spray and ensure thorough coverage of the plant surface. Spray until the foliage is well covered, without excessive runoff. Can Bloom Up be used through a drip system? Bloom Up is primarily designed for foliar application because its main action depends on forming a reflective film on plant surfaces. For best results, it should be sprayed onto the crop canopy. Use through drip or irrigation systems should only be considered where specifically validated for the crop and application system. Is Bloom Up biodegradable? Yes. Bloom Up is biodegradable and non-toxic, making it suitable for sustainable crop stress-management programs. Key Benefits Reduces Water Loss from Plant Surfaces Bloom Up forms a thin reflective film on plant surfaces, helping reduce water loss and lowering the impact of excess heat and sunlight on plant tissues. Supports Plants During Drought Stress By helping plants conserve moisture, Bloom Up supports better tolerance to drought, heat, and water-stress conditions. Helps Maintain Turgor and Freshness Bloom Up helps maintain relative water content and cell turgidity, supporting healthier-looking crops and better freshness after harvest. Improves Post-Harvest Quality Bloom Up can help improve post-harvest keeping quality and vase life by reducing moisture loss and supporting tissue firmness. Enhances Crop Health Bloom Up supports overall crop health by helping plants manage environmental stress while maintaining normal physiological activity. Suitable for a Wide Range of Crops Bloom Up can be used on cereals, pulses, oilseeds, vegetables, fruits, flowers, orchards, ornamentals, plantation crops, spices, medicinal crops, and aromatic crops. Non-Toxic and Biodegradable Bloom Up is non-toxic, biodegradable, and suitable for environmentally responsible crop management programs. Dosage & Application Dosage: Mix 3-5 ml of Bloom Up per 1 liter of water. Drip System: Prepare the spray solution by mixing at the recommended rate (3-5 ml / liter). Adjust the volume of spray fluid according to crop canopy and local practices. Foliar Application: Ensure thorough coverage until dripping point. Recommended dosage is for guideline purpose only. More effective application rates may exist depending on specific circumstances. Related Products Trichoderma viride Beauveria bassiana Flyban Insecta Repel Larvicare Mealycare Metarhzium Anisopliae Mitimax More Products Resources Read all

Flavobacterium oceanosedimentum is a marine bacterium commonly found in ocean sediments, where it plays a critical role in nutrient cycling and organic matter decomposition. This bacterium degrades complex organic materials, contributing to the recycling of nutrients essential for marine ecosystem health. Additionally, F. oceanosedimentum demonstrates potential in bioremediation, particularly in degrading hydrocarbons and other pollutants in marine environments. Its metabolic adaptability and ability to thrive in challenging sediment conditions make it a valuable organism for maintaining ecological balance and supporting sustainable marine resource management. < Microbial Species Flavobacter oceanosedimentum Flavobacterium oceanosedimentum is a marine bacterium commonly found in ocean sediments, where it plays a critical role in nutrient cycling and organic matter decomposition. This bacterium degrades complex organic materials, contributing to the recycling of nutrients essential for marine ecosystem health. Additionally, F. oceanosedimentum demonstrates potential in bioremediation, particularly in degrading hydrocarbons and other pollutants in marine environments. Its metabolic adaptability and ability to thrive in challenging sediment conditions make it a valuable organism for maintaining ecological balance and supporting sustainable marine resource management. Strength 1 x 10⁸ CFU per gram / 1 x 10⁹ CFU per gram / 1 x 10¹⁰ CFU per gram Product Enquiry Buy this species Download Brochure Benefits Nutrient Cycling Contributes to nutrient cycling in marine ecosystems, supporting the health of aquatic life. Hydrocarbon Degradation Capable of degrading hydrocarbons, making it valuable for addressing oil spills and related pollution. Marine Pollutant Degradation Effectively breaks down organic pollutants in marine environments, aiding in ocean cleanup efforts. Sediment Bioremediation Enhances the degradation of contaminants in ocean sediments, promoting ecosystem restoration. Dosage & Application Additional Info Scientific References Mode of Action Sustainability Advantage FAQ Scientific References Content coming soon! Mode of Action Content coming soon! Additional Info Contact us for more details Dosage & Application Contact us for more details FAQ Content coming soon! Sustainability Advantage Related Products Saccharomyces cerevisiae Bacillus polymyxa Thiobacillus novellus Thiobacillus thiooxidans Alcaligenes denitrificans Bacillus licheniformis Bacillus macerans Citrobacter braakii More Products Resources Read all

Premier manufacturer & exporter of Enzymax, offering cutting-edge, eco-friendly solutions for effective environmental management. < Environmental Solutions Enzymax Enzyme-based agent for decomposing tough biomass (crop residues, fruit waste), effective at low temperatures, safe for beneficial organisms, approved for organic agriculture. Product Enquiry Download Brochure Benefits Versatility in Temperature Works well in low temperature conditions unlike microbes, allowing for decomposition even in colder environments. Faster Decomposition Requires lesser reaction time compared to microbes at low temperatures, speeding up the decomposition process. Compatibility with Agricultural Chemicals Compatible with various agricultural chemicals, including weedicides, fungicides, and herbicides, without losing effectiveness. Efficient Decomposition Contains potent enzymes which efficiently degrade hard-to-digest material into organic fertilizer/compost. Composition Dosage & Application Additional Info FAQ Composition Components Enzymax comprises of unique enzymes that decompose cellulose, lignin, protein, lipids and all other associated debris matter. The composition is proprietary. Dosage & Application Dose: 1-2 L per Ha depending on crop residue volume Crops: All Crop residues, Straw Crop residue after harvest is left in the field. Dilute recommended quantity of Enzymax in sufficient water and spray on crop residue. Crop residue from crops such as cotton, sugarcane and banana can be pulverized and decomposed in off field sites by treating with Enzymax at a dose of 1 L / cubic metre of biomass. Note: Do not store Enzymax solution for more than 24 hours after mixing in water. Additional Info Our application rates are for guidelines only. Compatibility: Enzymax is compatible with Biofertilizers and Biopesticides. Enzymax is compatible with chemical pesticides. chemical fungicides, weedicides, herbicides and chemical fertilizers Mode of action: Enzymes are strong agents which can break down cellulose, lignin, lipids and protein. The organic acids and enzymes hydrolyze and decompose the biomass by breaking down the cell wall and aid in faster decomposition. How to use: Shake the bottle well before use. This product should be mixed with clean water in a plastic container as per the dosage instructions and thoroughly mixed before pouring into organic waste. Instructions to open: Open the bottle outdoors with care. Do not shake the bottle before opening. The bottle has a double seal system - an external black cap and a white inner plug with a nozzle in the center. After opening the black outer cap, pierce the inner plug in the middle using any pointed tool. The nozzle should create a small hole through which the liquid fertilizer can pour out. Usage and storage: Protect from direct sunlight and store in a dark, cool place between 5 to 25°C (40-77°F). Do not refrigerate or freeze. Keep the container tightly sealed after use. Keep away from children and pets. Do not inhale or ingest. FAQ What is Enzymax used for? Enzymax is an enzyme-based composting accelerator specifically designed for decomposing tough, resistant biomass materials that are difficult to break down through natural processes alone. It is primarily used for: Crop Residues: Straw, corn stalks, hay, and other fibrous agricultural waste Fruit and Vegetable Waste: Processing waste from fruit canneries, juice production, and vegetable packing facilities Woody Materials: Wood chips, sawdust, paper waste, and lignocellulosic biomass Food Processing Waste: Pulp, peels, and discarded produce from food industries Garden and Landscape Waste: Leaves, grass clippings, branches, and yard trimmings The product works by providing specialized enzymes that target and break down the complex polymers found in plant material—specifically cellulose, lignin, protein, and lipids—converting them into simpler compounds that microorganisms can readily consume. This accelerates the composting process, reducing decomposition time from months to weeks. Is Enzymax a probiotic? No, Enzymax is fundamentally different from a probiotic product, though the distinction can be subtle. Key Differences: Enzymax (Enzyme-Based Product) Contains directly active enzymes that catalyze biochemical reactions Works through enzymatic catalysis to break down organic molecules Does not require living microorganisms to function Acts as a biochemical tool that works immediately upon application Particularly effective at low temperatures where microbial activity is limited Proprietary enzyme composition optimized for specific substrates Probiotics/Microbial Inoculants (e.g., Compost Pro, Enriched Earth) Contain live microorganisms (bacteria, fungi, actinomycetes) Work through microbial metabolism and reproduction Require favorable conditions (moisture, temperature, aeration, nutrients) to establish colonies Take time to colonize the compost pile and multiply Produce enzymes as part of their metabolic activity Introduce entire microbial communities for ecosystem development When to Use Each: Enzymax: When you have recalcitrant materials (woody, high-lignin waste), lower temperatures, or need rapid initial breakdown Probiotics: When you want complete microbial ecosystem development, pathogen elimination through competition, and long-term compost maturity Combined Approach: Many professional composters use both—applying Enzymax for initial substrate breakdown, then introducing probiotic inoculants to colonize and stabilize the pile What are the benefits of taking Enzymax? The benefits of using Enzymax in your composting operation are substantial and multifaceted: Speed and Efficiency Reduces composting time from 3-6 months to 4-8 weeks Enzymatic application can reduce required retention time by 30-50% Faster substrate breakdown increases processing capacity without expanding infrastructure Superior Substrate Degradation Cellulases break down cellulose (the most abundant plant polymer) into simpler sugars (cellobiose and glucose) Hemicellulases target hemicellulose, which comprises 20-35% of plant cell walls Ligninolytic enzymes degrade recalcitrant lignin structures that naturally resist decomposition Proteases break down proteins into amino acids and peptides Lipases hydrolyze fats and oils into glycerol and fatty acids This comprehensive enzymatic arsenal ensures complete substrate utilization Low-Temperature Operation Functions effectively at ambient and cool temperatures (below 40°C) Eliminates the need to rely on thermophilic bacteria that require high temperatures to activate Ideal for composting in cool climates or seasons Reduces energy requirements for temperature maintenance Safety and Environmental Benefits Contains no harmful chemicals or synthetic additives Safe for beneficial organisms including earthworms, mycorrhizal fungi, and nitrogen-fixing bacteria Approved for organic agriculture systems Does not interfere with the establishment of natural microbial communities Biodegradable and environmentally safe Reduces emissions of methane and other greenhouse gases by accelerating decomposition Enhanced Compost Quality More complete breakdown of organic matter leads to better nutrient availability Final compost contains higher concentrations of plant-available nutrients Improves soil structure, water retention, and microbial diversity when incorporated into soil Produces compost free from phytotoxic (plant-toxic) compounds Results in a dark, crumbly, earthy-smelling finished product Cost and Resource Efficiency Reduces labor costs by shortening composting cycles Decreases facility space requirements (smaller piles, faster turnover) Minimizes land requirements for staging waste materials Reduces transportation costs through faster waste conversion to usable compost What is the best accelerant for composting? The "best" composting accelerant depends on your specific circumstances, materials, and goals. Here's a comprehensive comparison: Enzyme-Based Accelerants (like Enzymax) Strengths: Most effective for tough, fibrous, or woody materials (high cellulose/lignin) Work at low temperatures Rapid initial substrate breakdown Direct enzymatic action requires no lag time for microbial establishment Best For: Agricultural residues, wood chips, crop waste, cool-climate composting Limitations: Don't provide microbial ecosystem development or pathogen elimination Microbial Inoculants (Thermophilic Bacteria Consortia) Strengths: Complete microbial ecosystem development Generate high temperatures (55-70°C) for pathogen elimination Produce multiple enzymes adapted to available substrates Create mature compost with stable humic compounds Faster overall composting (28-35 days with quality inoculants) Best For: General-purpose composting, pathogen-laden materials, municipal waste Limitations: Require optimization of moisture, aeration, and C:N ratio; slower initial breakdown of recalcitrant materials Natural/DIY Accelerants (Finished Compost, Manure, Effective Microorganisms) Strengths: Cost-effective Already contain established microbial communities Provide both enzymes and living microbes Best For: Budget-conscious operations, when commercial products unavailable Limitations: Variable effectiveness, inconsistent composition, may introduce weeds or pathogens Optimal Strategy: The most effective approach uses a tiered acceleration system: Phase 1: Apply Enzymax to substrate high in cellulose/lignin to achieve 30-40% mass reduction within 1-2 weeks Phase 2: Introduce microbial inoculants once temperature naturally rises and initial substrate breakdown occurs Phase 3: Maintain moisture, aeration, and C:N ratio; let microbes finish humification over 4-6 weeks Result: Complete degradation, pathogen elimination, and mature compost in 8-10 weeks This combined approach leverages the strengths of both enzyme and microbial systems for superior results. What chemicals are used in composting? Composting can involve various chemical additives, ranging from natural amendments to synthetic compounds. Here's a comprehensive breakdown: Organic/Natural Amendments (Approved for Organic Agriculture) Lime (Calcium Carbonate): Raises pH in acidic compost, neutralizes excess ammonia, reduces odor; also provides calcium Sulfur (Elemental): Lowers pH in alkaline conditions, provides sulfur nutrient Rock Phosphate: Slow-release phosphorus source Bone Meal & Blood Meal: Nitrogen sources and phosphorus amendment Biochar: Improves moisture retention, enhances microbial activity, absorbs ammonia Zeolite & Clay Minerals: Absorb ammonia and excess moisture; regulate pH Enzyme-Based Additives (Enzymax Category) Cellulases: Cleave cellulose polymers into glucose Proteases: Break down proteins into amino acids Lipases: Hydrolyze lipids into glycerol and fatty acids Hemicellulases: Target hemicellulose polymers Ligninolytic Peroxidases & Laccases: Oxidize and depolymerize lignin structures Microbial Inoculants (Beneficial Microorganisms) Thermophilic Bacteria: Bacillus, Thermus, Geobacillus species Cellulolytic Fungi: Trichoderma, Aspergillus species Actinomycetes: Streptomyces species for humification Nitrogen-Fixing Bacteria: Enhance nitrogen content Chemical Additives (Industrial/Conventional Composting) Urea (NH₂CONH₂): Synthetic nitrogen source; high analysis (46-0-0 NPK) Ammonium Nitrate: Synthetic nitrogen; highly soluble Phosphoric Acid: Adjusts pH and provides phosphorus Ammonia: Adds nitrogen directly; increases temperature Potassium Chloride: Potassium source Guano (Natural but Concentrated): High-analysis nitrogen and phosphorus Biologically Active Compounds Humic Acids & Fulvic Acids: Already partially decomposed organic matter; enhances nutrient cycling Seaweed Extract: Provides trace elements and growth hormones Effective Microorganisms (EM): Multi-species consortia of bacteria, yeast, and phototrophs Specialty Additives Peat Moss or Coconut Coir: Carbon source, moisture retention Compost Tea: Aqueous extract containing dissolved nutrients and microbes Vermicompost: Worm-processed material; introduces beneficial microbes Mycorrhizal Inoculants: Fungal spores that colonize compost ecosystem Chemical Comparisons for Compost Quality: Component Organic/Natural Options Synthetic Options Effect on Compost Nitrogen Blood meal, manure, Enzymax Urea, ammonia, ammonium nitrate Speeds decomposition; excess causes ammonia loss Phosphorus Bone meal, rock phosphate, guano Phosphoric acid Improves nutrient content Potassium Wood ash, seaweed, kelp meal Potassium chloride Enhances finished compost quality pH Adjustment Lime, sulfur Phosphoric acid, ammonia Controls acidity/alkalinity Microbial Activity Biochar, zeolite, compost None equivalent Improves structure and microbial diversity Key Consideration: For organic certification, only natural and approved biological amendments (like Enzymax and most microbial inoculants) are permitted. Synthetic chemicals are restricted to conventional composting operations. What enzymes are involved in decomposition? Decomposition is orchestrated by a specialized consortium of enzymes produced by bacteria, fungi, and actinomycetes. Each targets specific substrate polymers: Primary Hydrolytic Enzymes (Break Down Plant Structures) Cellulases (EC 3.2.1.4 family) Function: Cleave β-1,4-glycosidic bonds in cellulose Products: Cellobiose (disaccharide) and glucose (monosaccharide) Mechanism: Three-enzyme system working synergistically: Endoglucanases : Cut randomly within cellulose chains Exoglucanases (Cellobiohydrolases) : Remove cellobiose units from chain ends β-Glucosidases : Complete hydrolysis to glucose Produced by: Trichoderma reesei (fungi), Bacillus species (bacteria), Streptomyces species (actinomycetes) Significance: Cellulose comprises 40-50% of plant dry matter; is the most abundant organic polymer on Earth Hemicellulases (Multiple enzyme families) Function: Degrade hemicellulose (xylans, mannans, arabinoxylans) Enzyme types: Xylanases : Attack xylan backbone (β-D-xylopyranosyl bonds) Mannanases : Cleave mannan polymers Arabinofuranosidases : Remove arabinose side chains Acetyl Esterases : Remove acetyl groups Products: Xylose, mannose, and other pentose sugars Significance: Hemicelluloses are 20-35% of plant cell walls; more easily degradable than cellulose Ligninolytic Enzymes (Oxidoreductases for Lignin Degradation) Function: Break down and oxidize the highly recalcitrant lignin polymer Primary enzyme types: Laccases (Laccase Multicopper Oxidases) : Catalyze oxidation of phenolic compounds; produced by white-rot fungi Lignin Peroxidases (LiP) : Use hydrogen peroxide to oxidize aromatic compounds and lignin fragments Manganese Peroxidases (MnP) : Oxidize manganese and lignin structures Dye-Decolorizing Peroxidases (DyP) : Attack highly oxidized phenolic substrates Unspecific Peroxygenases (UPO) : Broad-spectrum oxidation Mechanism: Oxidative depolymerization breaks carbon-carbon and ether bonds in lignin Produced by: White-rot fungi (Phanerochaete chrysosporium, Trametes versicolor, Pleurotus species), some bacteria (Bacillus cereus, Rhodococcus species) Significance: Lignin is the second most abundant biopolymer; extremely resistant to degradation Secondary Hydrolytic Enzymes (Process Breakdown Products) Proteases (Endopeptidases and Aminopeptidases) Function: Break down proteins and peptides into amino acids Mechanism: Endopeptidases : Cleave peptide bonds within protein chains Aminopeptidases : Remove amino acids sequentially from chain ends Carboxypeptidases : Remove terminal amino acids Products: Free amino acids, small peptides Produced by: Bacillus species, Pseudomonas species, most decomposing bacteria and fungi Significance: Proteins comprise 5-10% of plant biomass; nitrogen is limiting nutrient in compost Lipases (Serine Hydrolases) Function: Hydrolyze triglycerides and other lipids into glycerol and fatty acids Mechanism: Cleave ester bonds between glycerol backbone and fatty acid chains Products: Glycerol, monoglycerides, free fatty acids Produced by: Pseudomonas, Bacillus, and Candida species; various fungi Significance: Fats comprise 5-15% of some food waste; oil-based materials resist degradation Amylases (Glycoside Hydrolases) Function: Cleave α-1,4 and α-1,6 glycosidic bonds in starch and glycogen Mechanism: α-Amylase : Cleaves bonds randomly within starch chains β-Amylase : Removes maltose units from chain ends Glucoamylase : Completes hydrolysis to glucose Products: Glucose, maltose, dextrins Produced by: Bacillus species (especially Bacillus subtilis), Aspergillus species, Trichoderma species Significance: Carbohydrates are readily degradable and provide quick energy for rapid microbial growth Pectinases (Polygalacturonases and Pectin Esterases) Function: Degrade pectin (found in plant middle lamellae and cell walls) Mechanism: Cleave galacturonic acid polymers; remove methoxy and acetyl groups Products: Galacturonic acid, oligomers Produced by: Aspergillus, Penicillium, and Bacillus species Significance: Facilitate breakdown of fruit and vegetable waste Xylanases (Specific Hemicellulase Family) Function: Specifically target and cleave xylan (β-1,4-linked xylose polymer) Mechanism: Endoxylanases cut within chains; exoxylanases remove xylose units Products: Xylose oligomers and monomers Produced by: Trichoderma, Aspergillus, Bacillus species Significance: Xylans comprise 5-30% of plant cell walls Tertiary Enzymes (Nutrient Cycling & Stabilization) Phosphatases (Acid and Alkaline) Function: Release phosphate from organic phosphate compounds Products: Plant-available orthophosphate (PO₄³⁻) Significance: Improves phosphorus availability in finished compost Urease (Nitrogen Metabolism) Function: Hydrolyzes urea into ammonia and CO₂ Significance: Converts urea amendments into bioavailable nitrogen Catalase & Peroxidase (Oxidative Enzymes) Function: Decompose hydrogen peroxide and reactive oxygen species Significance: Protect cells from oxidative stress; indicate microbial vitality Enzymatic Succession During Composting Phases: Composting Phase Temperature Dominant Enzymes Function Psychrophilic (Startup) <20°C Amylase, protease, lipase Rapid breakdown of simple, readily available compounds Mesophilic (Acceleration) 20-40°C Cellulase, protease, amylase Active mass reduction; 50% substrate loss in 1-2 weeks Thermophilic (Peak) 40-70°C Cellulase, hemicellulase, ligninolytic enzymes Intensive degradation of recalcitrant materials; pathogen elimination Curing (Maturation) <40°C Ligninolytic peroxidases, secondary hydrolases Humification; stabilization into humic/fulvic acids Why Multiple Enzymes Are Required: Enzymatic degradation is not a sequential "assembly line" but a synergistic network where: Lytic Polysaccharide Monooxygenases (LPMOs) introduce breaks in crystalline cellulose, making it accessible to cellulases Hemicellulases expose cellulose microfibrils by removing surrounding hemicellulose Ligninolytic enzymes oxidize and depolymerize lignin, creating passages for bacterial penetration Proteases release amino acids that fuel thermogenesis and rapid microbial growth Lipases break down wax coatings on plant surfaces, improving overall substrate accessibility Enzymax provides a proprietary blend of these key enzymes in optimized ratios, allowing rapid substrate breakdown even when natural microbial populations are slow to establish. Enzymax stands apart from probiotic products by providing directly active enzymes rather than living microorganisms. It excels at decomposing tough plant materials—especially those high in cellulose and lignin—through enzymatic catalysis. While different from probiotics, Enzymax complements microbial inoculants perfectly in a comprehensive composting strategy. Understanding the specific enzymes involved in decomposition (cellulases, ligninolytic peroxidases, proteases, lipases, and many others) reveals why Enzymax's proprietary enzyme composition is specifically designed to accelerate the complex biochemical transformation of crop residues, fruit waste, and other challenging biomass into nutrient-rich, plant-available compost. Related Products Cellulomax Compost Pro Enriched Earth More Products Resources Read all

Rhizobium leguminosarum is a species of nitrogen-fixing bacteria that forms symbiotic relationships with leguminous plants, particularly peas, beans, and clover. These bacteria colonize the plant's root system and create nodules, where they convert atmospheric nitrogen (N₂) into ammonia (NH₃) through the enzyme nitrogenase. This process provides the plant with essential nitrogen, facilitating its growth while simultaneously improving soil fertility. Rhizobium leguminosarum plays a key role in sustainable agriculture by reducing the need for synthetic nitrogen fertilizers and enhancing crop yields naturally. < Microbial Species Rhizobium leguminosarum Rhizobium leguminosarum is a species of nitrogen-fixing bacteria that forms symbiotic relationships with leguminous plants, particularly peas, beans, and clover. These bacteria colonize the plant's root system and create nodules, where they convert atmospheric nitrogen (N₂) into ammonia (NH₃) through the enzyme nitrogenase. This process provides the plant with essential nitrogen, facilitating its growth while simultaneously improving soil fertility. Rhizobium leguminosarum plays a key role in sustainable agriculture by reducing the need for synthetic nitrogen fertilizers and enhancing crop yields naturally. Strength 1 x 10⁸ CFU per gram / 1 x 10⁹ CFU per gram / 1 x 10¹⁰ CFU per gram Product Enquiry Buy this species Download Brochure Benefits Phosphorus Solubilization Rhizobium leguminosarum increases phosphorus availability by converting insoluble phosphates intoc plant accessible forms. This enhances nutrient absorption , promotes vigorous plant growth , and elevates crop productivity . Stress Tolerance Rhizobium leguminosarum strengthens plant resilience against various abiotic stresses including drought, salinity, and nutrient scarcity, thereby enhancing crop performance under challenging environmental conditions. Enhanced Symbiosis Rhizobium leguminosarum establishes efficient symbiotic associations with diverse leguminous plants, significantly improving nitrogen fixation , stimulating robust root development , and maximizing overall crop yields . Disease Resistance By enhancing the health and microbial balance of the rhizosphere , Rhizobium leguminosarum actively contributes to disease suppression . It aids plants in resisting soil-borne pathogens , significantly reducing the prevalence of plant diseases . Dosage & Application Additional Info Scientific References Mode of Action Sustainability Advantage FAQ Scientific References Signaling in the Rhizobium-legume symbiosis Oldroyd, G. E., Murray, J. D., Poole, P. S., & Downie, J. A. (2011). Annual Review of Genetics , 45, 119-144. Link to Article Rhizobium–legume symbiosis and nitrogen fixation under severe conditions and in an arid climate Zahran, H. H. (1999). Microbiology and Molecular Biology Reviews , 63(4), 968-989. Link to Article Leghemoglobin and the oxygen diffusion barrier in root nodules Appleby, C. A. (1984). Annual Review of Plant Physiology , 35(1), 443-478. Link to Article Nitrogenase structure and function Hoffman, B. M., Lukoyanov, D., Yang, Z. Y., Dean, D. R., & Seefeldt, L. C. (2014). Chemical Reviews , 114(8), 4041-4062. Link to Article Reactive oxygen species in legume root nodules Puppo, A., Groten, K., Bastian, F., Carzaniga, R., Soussi, M., Lucas, M. M., & Harrison, J. (2005). Plant Physiology , 137(4), 1202-1209. Link to Article Mode of Action Mode of Action: Rhizobium leguminosarum Rhizobium leguminosarum employs a sophisticated mechanism of action to establish symbiotic relationships with leguminous plants, significantly contributing to plant growth and soil fertility. The process begins with the exchange of chemical signals between the plant roots and the bacteria. Flavonoids secreted by legume roots attract Rhizobium bacteria, which in response, produce Nod factors (lipochitooligosaccharides) crucial for initiating symbiosis. Upon recognition of Nod factors, root hairs begin to curl, forming structures that encapsulate the bacteria. These bacteria penetrate the root hair and multiply, triggering the formation of infection threads through which Rhizobium migrates towards the root cortex. Concurrently, cortical cells undergo rapid division, resulting in the formation of specialized structures called nodules. Schematic representation of establishment of legume-rhizobia symbiosis and biological nitrogen-fixation process in nodules Within these nodules, Rhizobium differentiates into a specialized form known as bacteroids. These bacteroids utilize the enzyme nitrogenase to catalyze the conversion of inert atmospheric nitrogen (N₂) into ammonia (NH₃), a form of nitrogen readily assimilated by the plant. This nitrogen fixation is energy-intensive, requiring significant ATP and electrons derived from plant photosynthesis. The enzyme nitrogenase is highly sensitive to oxygen; hence, the nodule environment is adapted to maintain low oxygen concentrations through the plant-derived protein leghemoglobin, facilitating optimal nitrogenase function. Additionally, Rhizobium leguminosarum is equipped with protective antioxidant systems such as glutathione peroxidase (Gpx), which mitigates oxidative stress by neutralizing reactive oxygen species (ROS) generated during high metabolic activity within nodules. This antioxidant activity is essential for efficient nodulation and nitrogen fixation, as oxidative stress can significantly impair bacterial survival and nodule functionality. Thus, Rhizobium leguminosarum’s mode of action encompasses chemical signaling, physical interaction with the host plant, differentiation into nitrogen-fixing bacteroids, maintenance of an oxygen-regulated microenvironment, and robust antioxidant protection. Collectively, these mechanisms underscore the bacterium’s critical role in sustainable agriculture through improved crop nutrition and soil health. Additional Info Recommended Crops: Cereals, Millets, Pulses, Oilseeds, Fibre Crops, Sugar Crops, Forage Crops, Plantation crops, Vegetables, Fruits, Spices, Flowers, Medicinal crops, Aromatic Crops, Orchards, and Ornamentals. Compatibility: Compatible with Bio Pesticides, Bio Fertilizers, and Plant growth hormones but not with chemical fertilizers and chemical pesticides. Shelf Life: Stable within 1 year from the date of manufacturing. Packing: We offer tailor-made packaging as per customers' requirements. Dosage & Application Seed Coating/Seed Treatment: 1 kg of seeds will be coated with a slurry mixture of 10 g of Rhizobium Leguminosarum and 10 g of crude sugar in sufficient water. The coated seeds will then be dried in shade and sown or broadcast in the field. Seedling Treatment: Dip the seedlings into the mixture of 100 grams Rhizobium Leguminosarum and a sufficient amount of water. Soil Treatment: Mix 3-5 kg per acre of Rhizobium Leguminosarum with organic manure/organic fertilizers. Incorporate the mixture and spread it into the field at the time of planting/sowing. Irrigation: Mix 3 kg per acre of Rhizobium Leguminosarum in a sufficient amount of water and run it into the drip lines. FAQ What is Rhizobium leguminosarum? Rhizobium leguminosarum is a species of nitrogen-fixing bacteria that forms symbiotic relationships with leguminous plants, such as peas, beans, lentils, and clover. It colonizes plant root nodules, converting atmospheric nitrogen into ammonia, which is readily usable by plants. How does Rhizobium leguminosarum benefit plant growth? Rhizobium leguminosarum significantly enhances plant growth by: Providing nitrogen directly to plants, reducing the need for chemical fertilizers. Increasing overall plant biomass and yield, especially in nitrogen-deficient soils. Producing growth-promoting substances like indole acetic acid (IAA), which further stimulate root development and enhance nutrient uptake. What role does Rhizobium leguminosarum play in soil health? Rhizobium leguminosarum contributes to soil health by: Improving soil fertility through the natural fixation of nitrogen. Enhancing soil structure by increasing root biomass and soil organic matter content. Supporting the activity of beneficial soil microorganisms, thereby promoting a healthy soil ecosystem. Read here for Rhizobium Species: Role in Plant Nutrition, Crop Quality, Soil biology and Climate Change Mitigation Potential. What ecological values does Rhizobium leguminosarum offer? Ecological benefits include: Reducing reliance on synthetic fertilizers, thus lowering agricultural chemical runoff and groundwater contamination. Promoting biodiversity by fostering sustainable agricultural practices. Contributing to carbon sequestration by increasing soil organic matter. Can Rhizobium leguminosarum protect plants against diseases or stress conditions? Yes, Rhizobium leguminosarum: Enhances plant resilience to abiotic stresses such as drought and salinity by improving root architecture and nutrient uptake. Indirectly contributes to plant disease resistance by improving plant vigor and stimulating defense mechanisms against pathogens. How can Rhizobium leguminosarum be effectively utilized in agriculture? Effective utilization strategies include: Seed inoculation with commercial Rhizobium leguminosarum formulations prior to planting legumes. Integrating crop rotation practices that include leguminous plants to maintain soil nitrogen levels naturally. Combining Rhizobium inoculation with other plant-growth-promoting microbes for synergistic effects. Sustainability Advantage Related Products Acetobacter xylinum Azospirillum brasilense Azospirillum lipoferum Azospirillum spp. Azotobacter vinelandii Beijerinckia indica Bradyrhizobium elkanii Bradyrhizobium japonicum More Products Resources Read all

< Animal Health Aquamin Aquamin is a specialized multi-mineral aquatic feed that is used for treating fishes & shrimps. It helps greatly in inducing moulting and maintains equilibrium in osmoregulation. Overall improvement in growth and survival rates of the treated fish. Product Enquiry Benefits Improves Moulting and Growth in Crustaceans Aids in proper moulting and supports shell development through an optimal mineral balance, improving growth and reducing deformities. Prevents Mineral Deficiencies and Health Issues Supplies essential minerals, nutrients, and amino acids required by fish and shrimp, helping to prevent loose shell, white muscle, muscle cramp, and imbalances in shell hardness. Strengthens Immunity and Osmoregulation Enhances immune response and supports a stable osmoregulation mechanism, helping aquatic species adapt to environmental fluctuations more effectively. Enhances Pond Productivity and Water Quality Optimizes feed conversion ratio, enriches both primary and secondary productivity, and helps maintain the ideal pH level in pond water to maximize overall aquaculture output. Component Amount per 100g Calcium 20% Phosphorus 12% Choline Chloride 1% Magnesium 5% Copper 0.10% Zinc 0.80% Manganese 0.12% Iodine 0.03% Iron 0.40% Cobalt 0.01% Selenium 0.00% Probiotics 3.5g Excipients add 100g Composition Distinction FAQ Additional Info FAQ Content coming soon! Dosage & Application Content coming soon! Additional Info Content coming soon! Related Products Piscicare Livcare Aquatract Aqua Energy Aqua Pro Probio Aqua More Products Resources Read all

Bradyrhizobium elkanii a bacterium that forms symbiotic relationships with legume roots, significantly improving nitrogen availability in the soil, which is essential for leguminous crop production. < Microbial Species Bradyrhizobium elkanii Bradyrhizobium elkanii a bacterium that forms symbiotic relationships with legume roots, significantly improving nitrogen availability in the soil, which is essential for leguminous crop production. Strength 1 x 10⁸ CFU per gram / 1 x 10⁹ CFU per gram / 1 x 10¹⁰ CFU per gram Product Enquiry Buy this species Download Brochure Benefits Nitrogen Fixation Bradyrhizobium elkanii forms symbiotic relationships with leguminous plants, fixing atmospheric nitrogen into ammonia, which enhances soil fertility and plant growth. Enhanced Nutrient Availability It enhances the availability of essential nutrients such as phosphorus and iron to the host plant, contributing to improved plant health and yield. Stress Tolerance Bradyrhizobium elkanii produces stress-protective compounds like exopolysaccharides, aiding plants in coping with environmental stresses such as drought and salinity. Biocontrol Agent It competes with pathogenic microorganisms in the rhizosphere, helping to suppress plant diseases and promote healthier plant growth. Dosage & Application Additional Info Scientific References Mode of Action Sustainability Advantage FAQ Scientific References Scientific References and Molecular Mechanisms of Symbiosis (2025 Update) Overview of Bradyrhizobium elkanii Symbiotic Signaling The establishment of B. elkanii-legume symbiosis is a sophisticated molecular dialogue involving plant-derived signals (flavonoids), bacterial Nod factors (NFs), Type III secretion system (T3SS) effectors, and host-encoded resistance proteins. This intricate regulatory network determines host specificity, nodule organogenesis, and nitrogen fixation efficiency. 1. Molecular Signaling Initiation Flavonoid-Mediated Activation Host-to-Bacterium Signal:Legume roots experiencing nitrogen starvation exude flavonoid compounds (e.g., genistein, daidzein, luteolin) into the rhizosphere. These flavonoids penetrate the B. elkanii cell membrane and bind to the NodD regulatory protein, a member of the LysR family of transcriptional regulators. Key Research Findings: Flavonoid concentrations as low as 10⁻⁸ M activate nod gene expression in B. elkanii Different legume species exude distinct flavonoid profiles, contributing to host specificity Transcription of the nodYABCSUIJnolMNOnodZ operon is directly dependent upon NodD-flavonoid complexes TtsI (transcriptional activator of T3SS) is also responsive to flavonoids and coordinates both Nod factor and T3SS expression Regulatory Architecture The B. elkanii regulatory circuit involves: NodD: LysR-type regulator controlling nod gene expression NodW: Regulatory protein modulating flavonoid recognition TtsI: Transcriptional regulator of T3SS genes, activated by plant flavonoids Coordination of these regulators ensures spatiotemporal expression of symbiotic genes 2. Nod Factor Biosynthesis and Host Recognition Structure and Function Nod Factors (NFs):Nod factors are lipochitooligosaccharides (LCOs) comprising a backbone of 3–5 N-acetyl-D-glucosamine (GlcNAc) units with a long-chain fatty acyl group (C16–C18) attached to the non-reducing terminus. Nod Gene Clusters in B. elkanii: nodA: Encodes N-acetyl transferase; transfers the acyl chain to the GlcNAc backbone nodB: N-acetyl lyase; removes N-acetyl group from the non-reducing terminus nodC: Chitin synthase; synthesizes the GlcNAc backbone nodS, nodU, nodI, nodJ: Involved in modification and transport of Nod factors nodZ: Encodes a glucosidase involved in Nod factor modification for B. elkanii-specific legume recognition Nod Factor Modification B. elkanii produces modified Nod factors unique to this species: Acetyl substitution patterns differ between strains Host-specific decorations on the oligosaccharide backbone determine compatibility with legume receptors (NFRs: Nod Factor Receptors) Molecular recognition is highly specific; B. elkanii NF structure triggers nodulation in soybean (Glycine max), but not in hosts compatible with other rhizobia Structural Variations and Host Specificity B. elkanii genomes harbor extensive nodulation gene repertoires: Multiple nod gene variants on symbiotic islands allow synthesis of a spectrum of Nod factor structures Comparative genomic analysis reveals gene duplications and deletions affecting Nod factor decoration These variations contribute to the competitive nodulation phenotype of B. elkanii and its ability to nodulate multiple legume hosts at variable efficiency 3. Type III Secretion System (T3SS) and Effector Proteins T3SS Architecture The T3SS is a molecular syringe-like apparatus embedded in the bacterial cell envelope that delivers effector proteins (Nops: nodulation outer proteins) directly into host plant cells. T3SS Components in B. elkanii: RhcJ: Outer membrane channel protein RhcV: Inner membrane channel protein RhcQ: ATPase providing energy for protein secretion RhcC, RhcD, RhcE, RhcF: Basal body proteins FlhA, FliK, FliP: Apparatus assembly proteins Transcriptional Control: T3SS gene expression is controlled by TtsI (transcriptional activator) TtsI is activated by plant flavonoids, creating a coordinated response with Nod factor synthesis The T3SS is activated only in the presence of compatible plant roots, preventing wasteful energy expenditure in the soil T3SS Effector Proteins and Functions NopL: Key Determinant for Nodule Organogenesis Function: NopL is among the most critical T3SS effectors, particularly for B. elkanii USDA61 symbiosis with certain legume species (e.g., Vigna mungo). NopL-deleted mutants form infection threads on Vigna mungo roots but fail to establish nodules, indicating its essential role in nodule primordia formation NopL is exclusively conserved among Bradyrhizobium and Sinorhizobium genera, suggesting ancient evolutionary origin Phylogenetic analysis indicates NopL diverged from the canonical T3SS lineage, suggesting specialized symbiotic function Mechanism: NopL enters host cell nuclei and likely interacts with plant transcription factors Suppresses host immune responses that would otherwise block infection Triggers expression of early nodulation genes required for meristem initiation Bel2-5: NF-Independent Nodulation Effector Dual Functions: In some legumes (e.g., soybean nfr1 mutants), Bel2-5 can trigger nodulation independently of Nod factors In soybean carrying the Rj4 allele (dominant resistance gene), Bel2-5 acts as a virulence factor, triggering immune responses that prevent infection Structural Features: Contains ubiquitin-like protease (ULP) domain Two EAR (ethylene-responsive element-binding factor-associated amphiphilic repression) motifs for transcriptional regulation Nuclear localization signal (NLS) enabling entry into plant cell nuclei Internal repeat sequences with unknown function Shares structural similarity with XopD from the plant pathogen Xanthomonas campestris pv. vesicatoria Domain-Function Correlation: The C-terminal ULP domain and upstream regions are critical for Bel2-5-dependent nodulation phenotypes Mutations in EAR motifs abolish nodulation ability Deletion of NLS impairs nuclear targeting and symbiotic function InnB: Strain-Specific Symbiotic Modulator Host-Specific Effects: InnB promotes nodulation on Vigna mungo cultivars InnB restricts nodulation on Vigna radiata cv. KPS1 This differential phenotype reflects distinct recognition mechanisms in different legume species Expression and Localization: innB expression is flavonoid-dependent and TtsI-regulated InnB protein is secreted via T3SS and translocated into host cells Adenylate cyclase assays confirm T3SS-dependent translocation into nodule cells NopM: Ubiquitin Ligase Triggering Senescence Function: NopM triggers early senescence-like responses in incompatible hosts (e.g., Lotus species). Possesses E3 ubiquitin ligase domain and leucine-rich-repeat domain Acts similarly to PAMP-triggered immunity (PTI) and effector-triggered immunity (ETI) in pathogenic bacteria Mediates ubiquitination of host target proteins, leading to degradation and immune responses Results in browning of nodules and disrupted symbiosis Phylogenetic Conservation: NopM homologs are found in both pathogenic and symbiotic bacteria, highlighting the evolutionary relatedness of virulence and symbiotic mechanisms NopF: Infection Thread Inhibitor Role in Host Specificity: NopF triggers inhibition of infection thread formation in Lotus japonicus Gifu Represents a post-recognition checkpoint for host-pathogen compatibility Allows alternative legume accessions (L. burttii, L. japonicum MG-20) to proceed with symbiosis, despite presence of NopF NopP2: Fine-Tuning Symbiotic Efficiency Function: NopP2 fine-tunes symbiotic effectiveness with Vigna radiata. Located within the symbiotic island near the nif cluster Differential effects depending on host genotype and strain background Contributes to variable nodulation phenotypes among B. elkanii strains 4. Host Specificity and Rj Gene-Mediated Resistance The Rj Gene System in Soybean Soybean (Glycine max) possesses a dominant host resistance system controlled by Rj (Rejection) genes that restrict nodulation by specific Bradyrhizobium strains. Rj4 Gene: Encodes a thaumatin-like protein (TLP), a member of the pathogenesis-related (PR-5) protein family Structurally similar to plant anti-fungal proteins Restricts nodulation by many B. elkanii strains, particularly Type B strains (e.g., USDA61) Soybean cultivars carrying Rj4 are incompatible with B. elkanii but compatible with Bradyrhizobium diazoefficiens USDA110 Rj2 Gene: Encodes a TIR-NBS-LRR protein (Toll-interleukin receptor/nucleotide-binding site/leucine-rich repeat) Represents a receptor-like immune protein structurally similar to plant R proteins for pathogen resistance Critical amino acid I490 (isoleucine) in Rj2 determines incompatibility with Bradyrhizobium diazoefficiens USDA122 Restricts specific rhizobial strains but allows infection by compatible strains Rj3 Gene: Restricts B. elkanii Type B strains (e.g., BLY3-8, BLY6-1, USDA33) despite allowing nodulation by B. japonicum USDA110 T3SS and its effectors are critical for Rj3-mediated incompatibility Mutations in T3SS components (TtsI, RhcJ) overcome Rj3 restriction, confirming T3SS involvement Gene-for-Gene Model of Symbiotic Specificity The B. elkanii-soybean system exemplifies a gene-for-gene interaction: Bacterial avirulence gene (avr): T3SS effector genes (e.g., nopL, bel2-5, nopM) function as avirulence determinants Plant resistance gene (R): Soybean Rj genes encode receptors recognizing effector-triggered immune responses Incompatibility occurs when bacterial effector matches soybean R gene recognition specificity Compatibility requires bacterial effectors that evade or suppress Rj-mediated immunity 5. Infection and Nodule Development Infection Thread Formation Stages: Pre-infection: Nod factors bind to NFR1/NFR5 receptors on legume root epidermis, activating early symbiotic signaling Infection initiation: B. elkanii invades through root hair curling (Nod factor-dependent) or via crack entry (T3SS-dependent in certain genotypes) Intercellular infection: Bacteria travel through infection threads (wall-bound tubular structures) into the cortex Release and bacteroid formation: Bacteria are released into cortical cells and enclosed within plant-derived peribacteroid membranes Role of T3SS in Infection Nod factor-independent nodulation: B. elkanii T3SS effectors (particularly Bel2-5) can trigger nodulation of soybean nfr1 mutants lacking functional Nod factor receptors Infection thread progression: T3SS suppresses plant defense responses (ROS production, ethylene synthesis) that normally block infection thread elongation Bacterial release: T3SS effectors facilitate bacterial transition from infection threads into cortical cells for bacteroid development Nodule Organogenesis and Development Transcriptional Reprogramming: B. elkanii T3SS effectors and Nod factors activate soybean early nodulation genes: ENOD40, ENOD93, NIN (Nodule Inception), NSP1, NSP2 These plant genes activate meristem-like programs in cortical cells, initiating nodule primordia Coordinated T3E activity (NopL, Bel2-5, NopP2) is essential for primordia formation Nodule Maturation: Infected cells undergo endoreduplication (multiple rounds of DNA replication without cell division) Cortical cells expand to accommodate dividing bacterial cells Peribacteroid membranes establish nutrient exchange compartments Gibberellin Role: B. elkanii synthesizes gibberellin precursor (GA₉) via cytochrome P450 monooxygenase Host soybean expresses GA 3-oxidases (GA3ox) within nodules, converting GA₉ to bioactive GA₄ GA₄ regulates nodule size, influences meristem bifurcation, and modulates senescence Higher GA levels correlate with increased nodule size and bacterial progeny, providing selective advantage to GA-producing strains 6. Nitrogen Fixation Biochemistry Nitrogenase Enzyme Complex Components: Component I (MoFe protein): Contains molybdenum and iron clusters Component II (Fe protein): Contains iron-sulfur cluster; transfers electrons to Component I Electron donors: Bacteroid respiration provides reducing power; organic acids (malate, α-ketoglutarate) drive electron transport Catalytic Reaction:[ \text{N}_2 + 8 e^- + 16 \text{ATP} \to 2 \text{NH}_3 + \text{H}_2 + 16 \text{ADP} + 16 P_i ] Key Features: Requires strictly anaerobic conditions (oxygen sensitivity) Demands substantial ATP input (~16 molecules ATP per N₂ molecule fixed) B. elkanii bacteroids express oxygen-scavenging mechanisms including leghemoglobin synthesis Oxygen Management in Nodules Oxygen Gradient: Outer nodule layers maintain aerobic respiration for ATP generation Interior nodule zones remain anaerobic for nitrogenase activity B. elkanii respiration consumes oxygen in bacterial layers, maintaining hypoxia in nitrogenase-active compartments Oxygen-Protective Mechanisms: Leghemoglobin (plant-encoded, bacteroid-synthesized iron-containing protein) buffers oxygen at nanomolar levels, preventing nitrogenase inactivation Bacteroid differentiation produces enlarged, polyploid cells with reduced permeability to oxygen Expressed late nodulation proteins (Nols) contribute to oxygen protection Metabolic Integration Carbon-Nitrogen Balance: Host plants provide carbohydrates (photosynthetically-derived organic acids) to bacteroids B. elkanii oxidizes organic acids via citric acid cycle and electron transport chains, generating ATP and reducing equivalents for nitrogenase Efficient strains (e.g., B. elkanii USDA76) show higher enzyme levels for Nod factor synthesis and metabolic integration Ammonia Utilization: Ammonia fixed by nitrogenase is rapidly assimilated via glutamine synthetase (GS) in bacteroids However, much ammonia is excreted to host cells, where plants incorporate it into amino acids (glutamine, aspartate) Plant cells return nitrogen to bacteroids as amino acids and organic compounds, establishing exchange equilibrium 7. Regulatory Networks and Gene Expression NifA-RpoN Regulatory Circuit NifA: Sigma-54-dependent transcriptional activator controlling expression of nitrogen fixation (nif) and related genes Activates nifHDK genes encoding nitrogenase structural proteins Responsive to oxygen levels; activated under microoxic conditions characteristic of nodule interiors Coordinates temporal expression of nif genes with nodule development progression RpoN: Sigma-54 RNA polymerase recognizing NifA-bound promoters Directs transcription from nif promoters bearing NifA-binding sites Links nitrogen fixation gene expression to nodule maturation stage GlnR Regulatory Protein Function: Controls nitrogen assimilation genes and cross-talks with symbiotic signaling Represses genes for nitrogen scavenging (e.g., ABC transporters) when ammonia is abundant Releases repression when ammonia becomes limiting, activating alternative nitrogen acquisition pathways Prevents metabolic conflict during high nitrogen fixation rates AdeR (Adenine Deaminase Regulator) Role: Modulates purine metabolism and symbiotic efficiency Controls genes involved in nucleotide synthesis Adjusted expression enables rapid bacterial replication in nodules while supporting biosynthesis of symbiotic proteins 8. Comparative Genomics: Symbiotic Island Architecture Symbiotic Island Composition B. elkanii genomes contain low GC-content regions (symbiotic islands) harboring symbiosis-essential genes: Island A (Main symbiotic island): ~690 kb Contains nod cluster: nodABC, nodD, nodZ, regulatory sequences Contains nif cluster: nifHDK, nifENX, fixABCX Contains fix genes (flavoproteins, cytochromes) for electron transport Island B (Small region): ~4–44 kb Variable across strains; minimal genes Island C: ~200–518 kb Contains additional metabolic and regulatory genes Variable gene content among B. elkanii strains Lateral Gene Transfer and Evolutionary Plasticity Pangenome Analysis: Bradyrhizobium pangenome: 84,078 gene families across species Core genome: 824 genes (essential cell processes) Accessory genome: 42,409 genes (including symbiotic, metabolic, stress response functions) B. elkanii genomes are moderately stable compared to highly plastic genomes of some Sinorhizobium species Genetic Variations: SNPs and indels in symbiotic islands correlate with symbiotic phenotype differences Polymorphisms in nif, fix, and nodulation regulatory genes drive intraspecific variation Integrative conjugative elements (ICEs) facilitate horizontal transfer of symbiotic genes between Bradyrhizobium strains 9. Stress Response and Environmental Adaptation Osmotic Stress Tolerance Mechanisms: Production of exopolysaccharides (EPS) and trehalose Upregulation of osmolyte synthesis under salt stress Maintenance of cell membrane integrity under water deficit Acid-Soil Adaptation pH Tolerance: Many B. elkanii strains tolerate pH 4.5–6.5, though optimal nodulation occurs at pH 6.0–7.5 Expression of acid-tolerance proteins enables survival in acidic soils Selection pressure in Brazilian Cerrado soils (naturally acidic) has generated acid-adapted B. elkanii strains Mode of Action Step-by-Step Nodulation Process Phase 1: Recognition and Signaling (Hours 0–12) Host root exudation of flavonoids B. elkanii perception and chemotaxis toward root Activation of nod gene transcription via NodD-flavonoid interaction Synthesis and secretion of Nod factors Nod factor recognition by plant NFR1/NFR5 receptors Initiation of early nodulation gene expression in plant Phase 2: Infection (Days 1–3) Root hair curling and bacterial microcolony formation Infection thread invasion through root epidermis T3SS-mediated suppression of plant defense responses Intercellular infection thread progression toward cortex Bacterial translocation into cortical cells Phase 3: Nodule Organogenesis (Days 3–7) Induction of cortical cell mitosis (meristem activation) Differentiation of nodule tissues (vascular bundle, infection zone) Bacterial release from infection threads Formation of peribacteroid membranes Nodule structure maturation Phase 4: Bacteroid Differentiation and Nitrogen Fixation (Days 7–21) B. elkanii endoreduplication and morphological differentiation Expression of nitrogenase (nif) and iron-sulfur cluster synthesis genes Establishment of microaerobic environment Initiation of nitrogen fixation Nitrogen transfer to host plant Phase 5: Sustained Symbiosis (Weeks 3–Harvest) Peak nitrogen fixation rates Continuous nitrogen supply to plant Bacterial maintenance and reproduction within nodules Age-dependent nodule senescence in late pod-fill stages Additional Info Recommended Crops: Cereals, Millets, Pulses, Oilseeds, Fibre Crops, Sugar Crops, Forage Crops, Plantation crops, Vegetables, Fruits, Spices, Flowers, Medicinal crops, Aromatic Crops, Orchards, and Ornamentals. Compatibility: Compatible with Bio Pesticides, Bio Fertilizers, and Plant growth hormones but not with chemical fertilizers and chemical pesticides. Shelf Life: Stable within 1 year from the date of manufacturing. Packing: We offer tailor-made packaging as per customers' requirements. Dosage & Application Crop Recommendations and Compatibility Compatible Legumes for B. elkanii Primary Hosts: Soybean (Glycine max) – highest efficiency and most extensively studied Peanut (Arachis hypogaea) – excellent nodulation; SEMIA 6144 strain widely used Mung Bean (Vigna radiata) – strain-dependent compatibility (USDA61 is incompatible with some cultivars) Black-Eyed Pea (Vigna unguiculata) – variable efficiency depending on strain Secondary Hosts (with strain-specific compatibility): Groundnut (Arachis hypogaea) Yard-long Bean (Vigna unguiculata subsp. sesquipedalis) Black Gram (Vigna mungo) – USDA61 strain shows exceptional specificity Broad Host Range (Associated Legumes): Various Vigna species Certain Vicia species Select native legume species Non-Host Associations (Growth Promotion Without Nodulation) B. elkanii can colonize grass roots and promote growth through: Production of plant growth hormones (IAA, gibberellins) Enhanced root development and mineral uptake Demonstrated effects on: white oats, black oats, ryegrass Associated References: Similar to Paenibacillus azotofixans, which also promotes non-legume growth through PGPR mechanisms, B. elkanii exhibits plant growth-promoting properties beyond nodulation. Compatibility with Agricultural Inputs Input Type Compatibility Notes Bio-Pesticides Compatible Use with caution; avoid simultaneous application with broad-spectrum fungicides Bio-Fertilizers Compatible Synergistic effects with phosphate-solubilizing bacteria (PSB) observed Plant Growth Hormones Compatible Enhanced effects when combined with IAA or gibberellin-producing organisms Chemical Fertilizers Incompatible Avoid high rates of urea; inhibit nodule formation and nitrogen fixation Fungicides (Broad-Spectrum) Incompatible Fungicides reduce bacterial viability; use selective agents or pre-inoculation strategies Herbicides Compatible (Selective) Most herbicides compatible; avoid herbicides with antimicrobial activity Insecticides Compatible (Most) Compatibility varies by class; pyrethroids and neonicotinoids generally safe Shelf Life and Storage Shelf Life: Stable for up to 1 year from manufacturing date under proper conditions Storage Temperature: Cool, dry conditions; maintain 4–15°C for extended viability Light Protection: Store away from direct sunlight (UV light reduces viability) Humidity: Keep in sealed containers to prevent moisture loss Monitoring: Check for discoloration, odor, or contamination before use; discard if compromised Dosage and Application Methods Seed Coating/Seed Treatment Protocol: Prepare slurry: Mix 10 g of Bradyrhizobium elkanii with 10 g crude sugar in sufficient water Coat 1 kg of seeds evenly with slurry mixture Dry coated seeds in shade before sowing (allow 2–3 hours) Sow treated seeds immediately or store in cool, dry conditions for up to 60–90 days (viability maintained with proper storage) Advantages: Simple, cost-effective, ensures bacterium-seed contact, minimal equipment Seedling Treatment (Nursery Application) Protocol: Mix 100 g of Bradyrhizobium elkanii with sufficient water Dip seedling roots into inoculant slurry for 5–10 minutes Transplant seedlings into field immediately Applications: Nursery-raised legumes (peanut, some vegetables); labor-intensive but ensures high infection rates Soil Application (Broadcasting) Protocol: Mix 3–5 kg per acre of Bradyrhizobium elkanii with organic manure or vermicompost Distribute mixture uniformly across field during land preparation Incorporate into soil by plowing or harrowing 2–3 weeks before sowing Alternatively, apply close to seeding for rapid root colonization Advantages: Builds soil population; benefits residual inoculum for crop rotations Rate: 3–5 kg/acre optimal for establishment of ~10⁷–10⁸ CFU/g soil Irrigation/Fertigation Application Protocol: Mix 3 kg per acre of Bradyrhizobium elkanii in water (1:10 ratio) Pass through 100-mesh filter to remove particles Apply via drip lines or sprinkler irrigation system Best applied in evening to reduce UV exposure Advantages: Reaches established root systems; applicable post-emergence; supports nodule maintenance Timing: Early vegetative stages (V2–V4) for maximum nodule formation FAQ General Biology and Function What makes Bradyrhizobium elkanii different from free-living nitrogen fixers like Paenibacillus azotofixans? Bradyrhizobium elkanii is a symbiotic nitrogen fixer that forms intimate associations with legume roots and establishes specialized nitrogen-fixing nodules. In contrast, Paenibacillus azotofixans is a free-living nitrogen fixer that operates independently in soil without forming nodules. B. elkanii achieves higher nitrogen fixation rates (100–300 kg N/ha/season) through symbiotic cooperation with host plants, whereas P. azotofixans supplies more modest benefits (20–50 kg N/ha depending on conditions). B. elkanii cannot infect non-legume hosts, while P. azotofixans benefits a broad range of crop species through general PGPR mechanisms. For legume cultivation, B. elkanii is the preferred choice due to superior nitrogen fixation efficiency. How does Bradyrhizobium elkanii survive in different soil conditions? B. elkanii survives through multiple strategies. As a non-spore-forming bacterium, it depends on competitive fitness and metabolic flexibility rather than dormancy. B. elkanii tolerates: Acidic soils (pH 4.5–6.5): Acid-adapted strains (e.g., from Brazilian Cerrado) have evolved acid-tolerance proteins Drought: Produces exopolysaccharides (EPS) and osmolytes for osmotic balance Salinity: Synthesizes antioxidant molecules and ionic homeostasis proteins Temperature fluctuations: Expresses heat-shock proteins and cold-adaptation proteins Nutrient starvation: Metabolic versatility supports survival on minimal carbon and nitrogen sources Survival in soils is enhanced by host plant association, which supplies carbohydrates and maintains favorable microenvironments within root nodules. Can Bradyrhizobium species work synergistically with other soil bacteria? Yes, synergistic effects are well-documented: Phosphate-solubilizing bacteria (PSB): Co-inoculation with PSB (e.g., Bacillus megaterium) enhances phosphorus availability, improving B. elkanii nodule formation and nitrogen fixation Azospirillum species: Co-inoculation of B. elkanii with Azospirillum brasilense produces superior soybean growth through complementary IAA production; IAA stimulates root growth, improving rhizobial infection Bacillus subtilis: Co-inoculation in saline-alkali soils increased soybean yield by 18% compared to B. elkanii alone Biofilm formation: In consortia, rhizobia establish biofilms on root surfaces, enhancing competition with native rhizobia and pathogenic microbes What is the optimal soybean genotype for B. elkanii nodulation? Optimal genotypes depend on strain compatibility with soybean Rj genes: Best compatibility: Non-Rj genotypes and Rj4-gene carriers (with compatible B. elkanii strains, but not USDA61) Poor compatibility: Rj3-genotype cultivars generally incompatible with B. elkanii Type B strains Strain-specific: B. elkanii strains vary in effectiveness with different cultivars USDA76, SEMIA 587, SEMIA 5019: Good nodulation on most soybean genotypes USDA61: Excellent on soybean but incompatible with Rj4 genotypes Elite strains (e.g., ESA 123): Superior performance in drylands Recommendation: For maximum nitrogen fixation, select cultivars without restrictive Rj genes and pair with adapted strain Agricultural Applications and Management Which crops benefit most from Bradyrhizobium elkanii application? All legume crops benefit, but effectiveness varies: Highest benefit: Soybean, peanut, mung bean (90–300 kg N/ha fixation) Good benefit: Black-eyed pea, groundnut, yard-long bean (100–200 kg N/ha) Situational benefit: Native legumes, forage legumes (highly variable) No benefit: Non-legume crops (though limited growth promotion observed with some grasses) Factors maximizing benefit: Presence of native rhizobial population <10⁴ CFU/g soil Absence of antagonistic soil microbes Compatible soybean genotype (for soybean) Adequate soil pH (5.5–7.5) Highest ROI crops: Soybean in virgin soils; peanut in semi-arid regions with drought-adapted strains How quickly can farmers expect to see results from Bradyrhizobium elkanii inoculation? Timeline: 1–2 weeks post-inoculation: Infection thread formation; root colonization progresses 2–4 weeks: Visible nodule appearance; initiation of nitrogen fixation 4–8 weeks: Peak nodulation and nitrogen fixation rates established 8–16 weeks (R1–R5 stages in soybean): Cumulative nitrogen benefit becomes apparent in plant biomass Harvest: Final yield difference becomes quantifiable Field observations: Early-inoculated plants show accelerated growth compared to uninoculated controls Root development superior within 3–4 weeks Leaf color and vigor improvements evident by 6–8 weeks Yield increase: 5–60% depending on initial soil population and environmental conditions Maximum benefit: Observed at crop maturity; early-season nodulation establishes sustained nitrogen supply for pod fill and grain development Is Bradyrhizobium elkanii compatible with other agricultural inputs? Compatibility Summary: ✓ Bio-pesticides: Compatible (exclude broad-spectrum fungicides) ✓ Bio-fertilizers & PSB: Highly compatible; synergistic effects ✓ Plant hormones (IAA, GA): Compatible; enhanced effects ✓ Herbicides: Most compatible; avoid antimicrobial formulations ✗ Chemical fertilizers: High nitrogen rates inhibit nodulation ✗ Broad-spectrum fungicides: Lethal to B. elkanii; use selective or post-inoculation application ✗ Chemical nematicides: Many reduce viability Recommendation: Apply B. elkanii as early as possible (seed or pre-plant soil); avoid fungicides during first 4–6 weeks post-inoculation. Nitrogen fertilizers should be minimal (<50 kg N/ha) to avoid suppression of nitrogen fixation. Environmental Impact and Sustainability Does Bradyrhizobium elkanii have any environmental risks? Safety Profile: Naturally occurring soil bacterium; non-pathogenic to plants and animals No environmental accumulation; subject to normal soil microbial turnover Approved for organic farming systems (non-GMO) Reduces synthetic fertilizer use, thereby lowering greenhouse gas emissions Environmental Benefits: Replaces ~100–300 kg N/ha of synthetic fertilizer per crop season Synthetic fertilizer production accounts for ~2% of global energy use; B. elkanii reduces this footprint Decreases soil contamination risk from excess nitrate leaching Improves soil carbon sequestration through enhanced root exudation and organic matter Potential concerns (minimal): If non-competitive strains displace native rhizobia (rare; native populations typically recover) Nodule senescence releases carbon; however, net soil carbon often increases due to residual legume biomass Overall: B. elkanii inoculation is environmentally sound and beneficial to soil ecosystems How does Bradyrhizobium elkanii contribute to sustainable farming? Sustainability Contributions: Nitrogen cycle restoration: Reduces dependence on Haber-Bosch synthetic nitrogen Soil health: Improves biological activity, organic matter, and aggregate stability Crop rotation benefits: Legume crops (with B. elkanii) replenish nitrogen for subsequent cereal crops; reduces fertilizer for following season by 30–50% Carbon footprint reduction: Avoids emissions from fertilizer production (~0.5 kg CO₂ per kg N eliminated) Resilience to climate variability: Nitrogen fixation continues under drought (strain-dependent) better than relying on soil nitrogen pools Economic sustainability: Inoculant cost (~$2–5 per hectare) << synthetic nitrogen fertilizer cost (~$15–40 per hectare) Broader implications: Integration of B. elkanii inoculation into farming systems supports UN Sustainable Development Goal 12 (Responsible Consumption and Production) and Goal 13 (Climate Action) Can Bradyrhizobium elkanii help with climate change mitigation? Direct contributions: Reduced N₂O emissions: Elite strains carrying N₂O reductase (nos genes) reduce soil N₂O emissions by ~70% compared to standard strains Fertilizer reduction: Each kilogram of synthetic nitrogen avoided saves ~5 kg CO₂ equivalent from production and transport Soil carbon sequestration: Enhanced root exudation and legume residue decomposition increases soil carbon stocks Example calculation: Soybean field (50 ha) with B. elkanii inoculation Replaces 100 kg N/ha with biological fixation Avoids: 5,000 kg CO₂ equivalent (from fertilizer production), 100 kg N₂O equivalent (20 kg CO₂ equivalent), 250 kg CO₂ (from transport/application) Total mitigation: ~5,370 kg CO₂ equivalent per season Product Selection and Application Strategies How should Bradyrhizobium elkanii products be stored? Storage Conditions: Temperature: 4–15°C (cool, dry storage) Light: Darkness (UV light reduces viability by ~50% per week) Humidity: Sealed containers; humidity <70% Duration: Up to 1 year from manufacturing date Storage best practices: Keep in original sealed containers Store in dedicated cool storage (not with agrochemicals or fertilizers) Avoid direct sunlight, heat exposure Do not refrigerate below 4°C (cold stress reduces viability) Check for discoloration, foul odor, or contamination before use Discard products exceeding shelf life or showing signs of degradation Pre-application checks: Verify CFU concentration (should be ≥10⁸ CFU/g) Confirm expiration date Check for clumping or separation (sign of degradation) What is the optimal application timing for Bradyrhizobium elkanii? Timing Strategy: Best: Seed treatment 3–14 days before sowing (allows infection thread formation before water stress from germination) Good: At-planting seed treatment (simultaneous with sowing) Acceptable: Soil application 2–3 weeks before sowing (establishes soil population) Last resort: Early V2–V4 application (later than ideal but still effective) Seasonal considerations: Spring planting: Warmer soils favor infection; apply when soil temperature ≥15°C Monsoon crops: Ensure good soil drainage; waterlogged soils reduce nodulation Dry seasons: Apply post-irrigation or pre-monsoon for optimal soil moisture Sequential plantings: If crop residue is retained (no-till), residual soil population often supports second-year crops; re-inoculation beneficial only if populations fall below 10⁴ CFU/g soil Can organic farmers use Bradyrhizobium elkanii? Organic Certification Status: Yes, fully approved for certified organic production Bradyrhizobium elkanii is a naturally occurring, non-GMO soil bacterium Meets IFOAM (International Federation of Organic Agriculture Movements) standards Complies with organic certification requirements (USDA National Organic Program, EU Organic Regulation, others) Organic system benefits: Eliminates synthetic nitrogen fertilizer requirement Supports crop rotation strategies Improves soil biological diversity Aligns with organic philosophy of biological nutrient cycling Recommendations for organic farmers: Use seed treatments rather than synthetic fungicide combinations Apply biological inoculants early (seed or pre-plant) Avoid synthetic fungicides during critical nodulation period (first 4–6 weeks) Incorporate into comprehensive organic management (crop rotation, adequate organic matter, proper pH) Connecting B. elkanii and P. azotofixans While Bradyrhizobium elkanii and Paenibacillus azotofixans represent distinct nitrogen-fixing strategies, both contribute to agricultural sustainability: Characteristic B. elkanii P. azotofixans Nitrogen fixation strategy Symbiotic (nodulation) Free-living soil Host range Legumes (highly specific) Broad host range (all crops) Nitrogen contribution 100–300 kg N/ha/season 20–50 kg N/ha/season Nodule formation Yes; essential No PGPR functions Limited (nodulation-focused) Multiple (IAA, GA, biocontrol) Best use Legume crops Non-legumes and supplementary legume inoculation Interaction Can compete for nodule occupancy Complementary; enhances B. elkanii effectiveness via IAA production Integrated Approach: In diversified farming systems, B. elkanii inoculant for legume crops followed by P. azotofixans for non-legume crops creates a comprehensive biological nitrogen management strategy. Conclusion Bradyrhizobium elkanii represents a cornerstone microorganism for sustainable legume production. Its sophisticated molecular mechanisms for host recognition, infection, and nitrogen fixation, combined with practical agricultural benefits, make it indispensable for modern sustainable agriculture. With proper strain selection, timing, and integration with complementary practices, B. elkanii inoculation can significantly improve crop yields, reduce fertilizer dependency, and enhance soil health across diverse agroecosystems. Sustainability Advantage Related Products Acetobacter xylinum Azospirillum brasilense Azospirillum lipoferum Azospirillum spp. Azotobacter vinelandii Beijerinckia indica Bradyrhizobium japonicum Gluconacetobacter diazotrophicus More Products Resources Read all

Leading manufacturer & exporter of Mykrobak O&G, a natural, eco-friendly solution for soil & wastewater treatment, ensuring sustainability & efficiency. < Environmental Solutions Mykrobak O&G Mykrobak O&G is a biotechnological solution with potent microbes for breaking down oil and grease, including petroleum hydrocarbons. Product Enquiry Download Brochure Benefits Non-Toxic and Biodegradable Eco-friendly and biodegradable formulation that is harmless to humans and animals. Effective Dispersion Can be dispersed using fogging units to cover large areas effectively, especially in environments where noxious gases may build up. Versatile Odor Neutralization Neutralizes a wide range of odors including hydrogen sulfide, ammonia, amines, mercaptans, and more in various environments. Wide Application Suitable for use in wastewater treatment plants, petrochemical industry, food processing plants, composting yards, municipal dumping grounds, and public toilets. Composition Dosage & Application Additional Info FAQ Composition Dosage & Application Direction to use 1 litres of Mykrobak O&G need to be activated with the help of 50 litres of water and hold for 30 minutes for the deactivation of preservatives. Dosing It depends on the volume of waste water and oil & grease content in the waste water Additional Info Bacterial consortium belongs to the following: Hydrocarbon-reducing bacteria Hydrolytic bacteria Hyperthermophilic and thermophilic bacteria Nitrifying and denitrifying bacteria Photosynthetic bacteria & fluorescent bacteria Fermentative bacteria Acetogenic bacteria Odour control bacteria Enzymes belong to the co-enzymes of the following groups: Oxidoreductases Transferases Lyases Advantages of Mykrobak products: Promote the formation of potential and sustainable biomass Reduce contaminants, toxicity, pollutants, and bad odors Initiate biodegradation quickly Effective in reducing COD/BOD in ETP/STP/WTP Help in the fastest commissioning of biological treatment processes in ETP/STP, etc. Boost MLSS production rapidly Reduce ammoniacal nitrogen Improve digester system recovery Increase the efficiency of biogas production Improve tertiary treatment Reduce large quantities of organic compounds Improve the aquatic environment Clarify ponds and lakes water Safe and natural Economically feasible FAQ Content coming soon! Related Products Mykrobak Aerobic Mykrobak Anaerobic Wastewater Treatment Mykrobak Biotoilet Mykrobak Composting Mykrobak Dairy Mykrobak Drop Mykrobak Fog Mykrobak N&P Booster More Products Resources Read all

Hirsutella Thompsonii is a beneficial fungus used to control various small arachnids such as mites. It produces spores that penetrate the mite's cuticle, leading to paralysis and death. < Microbial Species Hirsutella thompsonii Hirsutella Thompsonii is a beneficial fungus used to control various small arachnids such as mites. It produces spores that penetrate the mite's cuticle, leading to paralysis and death. Strength 1 x 10⁸ CFU per gram / 1 x 10⁹ CFU per gram Product Enquiry Buy this species Download Brochure Benefits Effective mode of action Infects mites through their body contact, leading to paralysis and death. Rapid population reduction Quickly reduces and eliminates mite populations without developing resistance. Environmentally friendly Hirsutella thompsonii is safe for the environment and non-target organisms. High specificity Targets a range of mites and small arachnids while being safe for other organisms. Dosage & Application Additional Info Scientific References Mode of Action Sustainability Advantage FAQ Scientific References Sreerama Kumar P, Singh L. Enabling mycelial application of Hirsutella thompsonii for managing the coconut mite. Experimental and Applied Acarology , 2009;46(1–4):169–182. DOI: 10.1007/s10493-008-9177-3 Mazet I, Vey A. Hirsutellin A, a toxic protein produced in vitro by Hirsutella thompsonii . Microbiology , 1995;141(6):1343–1348. DOI: 10.1099/13500872-141-6-1343 Herrero-Galán E, Lacadena J, Martínez del Pozo Á, Boucias DG, Olmo N, Oñaderra M, Gavilanes JG. The insecticidal protein hirsutellin A from the mite fungal pathogen Hirsutella thompsonii is a ribotoxin. Proteins , 2008;72(1):217–228. DOI: 10.1002/prot.21910 Herrero-Galán E, García-Ortega L, Olombrada M, Lacadena J, Martínez del Pozo Á, Gavilanes JG, Oñaderra M. Hirsutellin A: a paradigmatic example of the insecticidal function of fungal ribotoxins. Insects , 2013;4(3):339–356. DOI: 10.3390/insects4030339 — free full text Maimala S, Tartar A, Boucias D, Chandrapatya A. Detection of the toxin hirsutellin A from Hirsutella thompsonii . Journal of Invertebrate Pathology , 2002;80(2):112–126. DOI: 10.1016/s0022-2011(02)00123-4 Rossi-Zalaf LS, Alves SB. Susceptibility of Brevipalpus phoenicis to entomopathogenic fungi. Experimental and Applied Acarology , 2006;40(1):37–47. DOI: 10.1007/s10493-006-9024-3 Peng CYS, Zhou X, Kaya HK. Virulence and site of infection of the fungus, Hirsutella thompsonii , to the honey bee ectoparasitic mite, Varroa destructor . Journal of Invertebrate Pathology , 2002;81(3):185–195. DOI: 10.1016/s0022-2011(02)00188-x Rosas-Acevedo JL, Boucias DG, Lezama R, Sims K, Pescador A. Exudate from sporulating cultures of Hirsutella thompsonii inhibit oviposition by the two-spotted spider mite Tetranychus urticae . Experimental and Applied Acarology , 2003;29(3–4):213–225. DOI: 10.1023/a:1025801817004 Omoto C, McCoy CW. Toxicity of purified fungal toxin hirsutellin A to the citrus rust mite Phyllocoptruta oleivora (Ash.). Journal of Invertebrate Pathology , 1998;72(3):319–322. DOI: 10.1006/jipa.1998.4813 Gerson U, Gafni A, Paz Z, Sztejnberg A. A tale of three acaropathogenic fungi in Israel: Hirsutella , Meira and Acaromyces . Experimental and Applied Acarology , 2008;46(1–4):183–194. DOI: 10.1007/s10493-008-9202-6 Nuvoloni FM, de Castro EB, Feres RJF. The role of native vegetation on infection rates of Calacarus heveae (Acari: Eriophyidae) by Hirsutella thompsonii (Ascomycota: Ophiocordycipitaceae). Experimental and Applied Acarology , 2014;63(2):157–169. DOI: 10.1007/s10493-014-9771-5 Demite PR, Feres RJF. Seasonality of pathogenic fungi in mites of rubber tree plantations adjacent to fragments of Cerrado. Brazilian Journal of Biology , 2008;68(3):535–538. DOI: 10.1590/s1519-69842008000300010 Kanga LHB, James RR, Boucias DG. Hirsutella thompsonii and Metarhizium anisopliae as potential microbial control agents of Varroa destructor , a honey bee parasite. Journal of Invertebrate Pathology , 2002;81(3):175–184. DOI: 10.1016/s0022-2011(02)00177-5 Smith KE, Wall R, French NP. The use of entomopathogenic fungi for the control of parasitic mites, Psoroptes spp. Veterinary Parasitology , 2000;92(2):97–105. DOI: 10.1016/s0304-4017(00)00277-6 Bałazy S, Wrzosek M, Sosnowska D, Tkaczuk C, Muszewska A. Laboratory trials to infect insects and nematodes by some acaropathogenic Hirsutella strains. Journal of Invertebrate Pathology , 2008;97(2):103–113. DOI: 10.1016/j.jip.2007.08.006 Savi PJ, de Moraes GJ, Hountondji FCC, Nansen C, de Andrade DJ. Compatibility of synthetic and biological pesticides with a biocontrol agent Phytoseiulus longipes (Acari: Phytoseiidae). Experimental and Applied Acarology , 2024;93(2):273–295. DOI: 10.1007/s10493-024-00926-3 — free full text Liu WZ, Boucias DG, McCoy CW. Extraction and characterization of the insecticidal toxin hirsutellin A produced by Hirsutella thompsonii var. thompsonii . Experimental Mycology , 1995;19(4):254–262. DOI: 10.1006/emyc.1995.1032 Boucias DG, Farmerie WG, Pendland JC. Cloning and sequencing of cDNA of the insecticidal toxin hirsutellin A. Journal of Invertebrate Pathology , 1998;72(3):258–261. DOI: 10.1006/jipa.1998.4762 Peer-reviewed sources were located via PubMed. Free full-text links point to PubMed Central where an open-access version is available. Mode of Action Content coming soon! Additional Info What this organism is Hirsutella thompsonii Fisher is a naturally occurring fungal pathogen of mites — an acaropathogen , meaning a fungus whose natural hosts are mites rather than insects. It is classified in the Ascomycota, family Ophiocordycipitaceae [11] . It is not a newly discovered organism. H. thompsonii is found naturally in mite populations across tropical and subtropical growing regions, and it is a well-documented cause of natural epizootics — population crashes — in eriophyid mites on citrus, coconut, rubber and other crops [11] [12] . Products based on it have been developed and used commercially since the 1980s, which makes it one of the longest-studied mycoacaricides. Documented host range Evidence is strongest for eriophyid and tenuipalpid mites: Mite group Example species studied Evidence Eriophyidae Citrus rust mite ( Phyllocoptruta oleivora ) The natural host from which the fungus was first characterized; purified fungal toxin reached almost 100% mortality in leaf bioassays at 100 µg/mL [9] Eriophyidae Coconut mite ( Aceria guerreronis ) Field application of a mycelial formulation reduced post-treatment mite populations by 85.6–97.1% [1] Eriophyidae Rubber tree mites ( Calacarus heveae , Phyllocoptruta seringueirae ) Natural infection documented; strongly seasonal [11] [12] Tenuipalpidae False spider mite ( Brevipalpus phoenicis ) Most virulent of 52 fungal isolates screened; mortality above 90% at 10⁷ conidia/mL [6] Tetranychidae Two-spotted spider mite ( Tetranychus urticae ) Culture exudate suppressed female fecundity by 100% over the first six days in laboratory assay [8] Varroidae Varroa destructor (honey bee mite) Laboratory and observation-hive studies; note that only 3 of 9 isolates tested were infectious [7] [13] Host range is not unlimited. In one in-vitro evaluation against the sheep and rabbit scab mite Psoroptes ovis , H. thompsonii showed no pathogenicity at all, while Metarhizium anisopliae did [14] . Susceptibility has to be established for the specific mite you are targeting. Why strain identity matters Performance varies substantially between isolates of the same species. In a screen of H. thompsonii isolates against Varroa destructor , only three of nine were infectious, and among those three the time to 50% mortality ranged from 52.7 to 96.7 hours [7] . Molecular work has also found H. thompsonii to be a genetically heterogeneous species [15] . Published results for one isolate do not establish the same performance for another. Formulation and storage Formulations: wettable powder (1 × 10⁸ CFU/g) and soluble powder (1 × 10⁹ CFU/g) Packaging: tailor-made to customer requirement Shelf life: stable for 1 year from the date of manufacture under the stated storage conditions Storage: cool, dry conditions, out of direct sunlight; do not store prepared spray solution for more than 24 hours Because this is a living organism, storage conditions directly determine what reaches the field. Heat and prolonged sunlight during storage or transport reduce viable propagule counts. Where it fits Hirsutella thompsonii is used as a component of an integrated mite-management program alongside monitoring, cultural practice, conservation of natural enemies and, where required, conventional acaricides applied on a resistance-management schedule. It is not a substitute for monitoring, and it is not positioned as a stand-alone replacement for every acaricide application. Dosage & Application Rates below are taken from the current product page. Confirm against the approved market-specific label before publication — see open items 1 and 2. Application rates Formulation Potency Foliar rate Water volume Frequency Wettable powder 1 × 10⁸ CFU/g 2 kg per acre (5 kg per hectare) Sufficient for full wetting of the infested plant part Two applications per season for perennial crops Soluble powder 1 × 10⁹ CFU/g 200 g per acre (500 g per hectare) Sufficient for full wetting of the infested plant part Two applications per season for long-duration crops Water volume is not fixed by the rate. What matters is that the spray reaches the mites, which on most crops means high-volume, high-coverage application rather than a concentrated low-volume pass. When to apply Hirsutella thompsonii is a living fungus that has to reach the mite, germinate on its cuticle and grow into it. Timing is therefore not a calendar decision — it is a decision about mite stage, weather, and canopy conditions. Apply at the first confirmed sign of mite activity, not after visible crop damage. Mite populations are far easier to hold down while they are building than after they have peaked. Time applications to humid periods. In coconut systems the conventional pattern is one application before the onset of monsoon and one after. In rubber plantations in Brazil, natural infection of pest mites by H. thompsonii peaked during the rainy season and fell below 5% in the dry season, which is why the authors described it as suited to rainy-season use [12] . Spray in the early morning or late afternoon. Conidia need moisture to germinate and are damaged by strong sunlight; simulated sunlight reduced conidium production by H. thompsonii in laboratory testing [1] . Avoid application immediately before heavy rain, which washes the inoculum off the leaf or fruit surface before it can establish. Repeat as the monitoring data requires. A second application is normally needed where mite pressure continues, because the fungus does not act instantly — see the response-time note below. Mixing and spray preparation Prepare the spray fresh and use it within 24 hours of mixing , as stated on the product label. Keep the spray tank out of direct sun while mixing and during application. Agitate continuously so the suspension does not settle. Do not use chlorinated water without checking it first; free chlorine can reduce viability of the propagules. Clean the tank of previous chemical residues, particularly fungicide residues, before loading. Check spray-water pH and hardness against the label requirements. Coverage Eriophyid mites are small and often sheltered. Coconut mite ( Aceria guerreronis ) lives under the fruit perianth; citrus rust mite lives on the fruit and leaf surface; tarsonemid and tetranychid mites concentrate on the underside of leaves. Direct the spray at the part of the plant the mite actually occupies , not simply at the canopy. Coverage failure is one of the most common reasons a biological acaricide underperforms. How quickly to expect a response This is not a knockdown product. Infection develops over days, not hours. Under laboratory conditions on the false spider mite Brevipalpus phoenicis , conidia were attached to the mite cuticle at 24 hours, colonization was evident by 48 hours, and the fungus produced new conidia on the cadaver at 120 hours [6] . On Varroa destructor , the time to 50% mortality ranged from 52.7 to 96.7 hours depending on which isolate was used [7] . Assess results five to seven days after application, not the next day. Before scale-up Any rate not printed on a registered label for your market is a starting point for controlled trial design, not a registered label recommendation. Confirm compatibility, viability after mixing, plant safety and performance on a small block before commercial use. Results may vary with mite species, crop, canopy, climate, application method, water quality and management. Use only in accordance with the current product label and local requirements. Dosage & Application Rates below reflect the formulation grades currently listed on the product page (1×10⁸, 1×10⁹ and 1×10¹⁰ CFU/g). Confirm every figure against the current approved market-specific label before publication — see open items 1 and 2. Application rates Formulation Potency Foliar rate Soil application Water volume Frequency Wettable powder 1 × 10⁸ CFU/g 2 kg/acre (5 kg/hectare) 2–5 kg/acre (5–12.5 kg/hectare) Sufficient for full coverage of the infested plant part Two applications per season for long-duration crops (pre- and post-monsoon) Soluble powder 1 × 10⁹ CFU/g 200 g/acre (500 g/hectare) 200–500 g/acre (500 g–1.25 kg/hectare) Sufficient for full coverage of the infested plant part Two applications per season for long-duration crops Soluble powder (high potency) 1 × 10¹⁰ CFU/g Technical powder - Generally not used in the field at this concentration - — — Rates on registered labels are set per market and per crop; the table above is the rate structure currently published on the site, not a substitute for the approved label in your target market. When to apply Lecanicillium lecanii is a living fungus. It has to land on the pest, germinate, and penetrate the cuticle before it does anything — so timing is a decision about pest stage and canopy humidity, not a fixed calendar date. Target the young stages. Susceptibility is not uniform across the pest's life cycle. In whitefly, second-instar nymphs were the most susceptible life stage tested, followed by third-instar nymphs, in a study on Bemisia tabaci Q-biotype [1] . Scout for nymphs and time the first application to when young nymphs are present rather than waiting for visible adult flight activity. Spray into humid conditions, not a dry spell. Every step of infection — conidial attachment, germination, cuticle penetration — is a moisture-dependent process on an exposed leaf surface. Published screening of entomopathogenic fungi for aphid control found the highest virulence of a L. lecanii strain at 75% relative humidity and 25°C, with control values near 100% within two to five days; the same strain still outperformed the other fungi tested at a lower 45% RH, but humidity remained a first-order variable in every trial in that study [2] . Early morning or evening application, when leaf surfaces retain moisture longest, is preferable to a midday spray into low humidity. Match temperature to the biology. L. lecanii is mesophilic, with optimum growth around 25°C and conidial germination reported across a broad 15–30°C range; one screened strain was also able to germinate at 35°C, wider than several other isolates tested in the same study [2] . Very cool or very hot spray windows will slow both germination and pest kill. Do not expect a fast knock-down. Infection is a multi-day process. In an ultrastructural study on the mealybug Phenacoccus fraxinus , conidia had attached and begun penetrating the cuticle by 24 hours, and internal tissues (fat body, trachea, muscle, nerve tissue) were being colonized by 48 hours [3] . Assess results several days after application, not the next day. Repeat applications as monitoring data requires, and follow the biannual (pre- and post-monsoon) pattern shown above for long-duration crops. Mixing and spray preparation Prepare the spray fresh and use it within 24 hours of mixing. Keep the spray tank out of direct sun while mixing and during application; sunlight and desiccation both reduce propagule viability on the leaf surface. Agitate continuously so the suspension does not settle. Treat fungicides as incompatible unless a specific test says otherwise. L. lecanii is a fungus, and it has been documented as highly sensitive to fungicide chemistry. In a 2023 study, wild-type Akanthomyces (Lecanicillium) lecanii strains were sensitive to the triazole fungicide tebuconazole at agriculturally recommended field doses; the authors had to develop UV-mutagenized, fungicide-resistant strains specifically because the wild-type organism was not tolerant of it [4] . This is the single most important compatibility caution on this page — clean the tank of fungicide residues before loading, and do not tank-mix with a fungicide unless it has been tested against the specific product batch. Check spray-water pH, hardness and residual chlorine against the label requirements; chlorinated water can reduce propagule viability in microbial products generally. Coverage Whiteflies and aphids cluster on the underside of leaves and around new growth. Direct the spray at the underside of the canopy and the growing points , not just the top of the plant. Coverage failure — spraying the upper canopy while the pest colonizes leaf undersides — is one of the most common reasons a biological insecticide underperforms in the field. Before scale-up Any rate not printed on a registered label for your market is a starting point for controlled trial design, not a registered label recommendation. Confirm compatibility, viability after mixing, plant safety and performance on a small block before commercial use. Results may vary with pest species, life stage, crop, canopy, climate, application method, water quality and management. Use only in accordance with the current product label and local requirements. FAQ What is Hirsutella thompsonii and why is it used as a mycoacaricide? Hirsutella thompsonii Fisher is a fungus that naturally infects and kills mites. "Mycoacaricide" simply means a mite-control product based on a fungus. It is used because it is one of the few fungal pathogens whose natural hosts are plant-feeding mites rather than insects, because it causes natural population crashes in eriophyid mites in the field, and because it has a mode of action unrelated to synthetic acaricides. It belongs to the Ascomycota, family Ophiocordycipitaceae [11] . Does Hirsutella thompsonii control coconut eriophyid mite ( Aceria guerreronis )? Coconut mite is one of the best-documented targets. In a field study in India, a mycelial formulation of H. thompsonii tank-mixed with adjuvants reduced post-treatment coconut mite populations by 85.6–97.1%. Palms treated with the fungus plus glycerol showed a mean pre-harvest nut damage grade of 2.0, against 4.0 in untreated control palms [1] . Two boundaries on that result. It was obtained with a specific mycelial formulation and specific adjuvants, and coconut mite lives under the fruit perianth, which makes spray coverage the limiting factor in practice. Results depend heavily on getting inoculum into that sheltered space. How does Hirsutella thompsonii infect and kill mites? By direct penetration of the cuticle, followed by internal colonization — not by ingestion, and not by contact paralysis. Scanning electron microscopy of the infection sequence on Brevipalpus phoenicis showed conidia attached to the mite integument within 24 hours, germinating and penetrating at the base of the setae on the hysterosoma; colonization was evident by 48 hours, and mycelium and conidiophores emerged from the cadaver by 120 hours [6] . On Varroa destructor , the point of entry was identified as the membranous arolium of the leg sucker [7] . The fungus also produces hirsutellin A (HtA) , a 15 kDa protein toxin [2] that has been characterized as a ribotoxin — it inactivates ribosomes by cleaving a single specific bond in ribosomal RNA, shutting down protein synthesis in the host [3] [4] . Purified HtA reached close to 100% mortality of adult citrus rust mite at 100 µg/mL in leaf bioassays, and reduced egg-laying before the mites died [9] . Importantly, the toxin is not the whole story. A survey of 162 H. thompsonii strains found the HtA gene in 100 of them, but its presence was not associated with greater insecticidal activity — strains with and without an amplifiable HtA gene produced filtrates causing the same average mortality. The authors concluded that HtA is required for neither survival nor pathogenicity, and that other, uncharacterized factors are involved [5] . A separate study found that an exudate from sporulating cultures suppressed spider mite egg-laying through a factor distinct from HtA [8] . Which types of mites are most commonly targeted by Hirsutella thompsonii ? Chiefly three families: Eriophyidae (rust, bud, gall and blister mites) — citrus rust mite, coconut mite, rubber tree mites. This is where the evidence is strongest [1] [9] [12] Tenuipalpidae (false spider mites) — Brevipalpus phoenicis , where H. thompsonii was by far the most virulent of 52 fungal isolates screened [6] Tetranychidae (spider mites) — effects on Tetranychus urticae are documented, including strong suppression of egg-laying [8] It has also been studied against the honey bee parasite Varroa destructor [7] [13] . It is not universally pathogenic to mites: against the scab mite Psoroptes ovis it showed no pathogenicity in laboratory testing [14] . Why do humidity, temperature and sunlight affect Hirsutella thompsonii performance? Because every step of infection is a biological process happening on an exposed leaf or fruit surface. Humidity is the critical one. A conidium must take up water to germinate before it can penetrate the mite. This requirement is well enough established to have decided the outcome of a commercial program: in Israel, H. thompsonii was introduced twice against citrus rust mite and killed 80–90% of exposed mites, but "due to its requirements for near-saturation humidities was deemed unsuitable for local outdoors conditions" [10] . That is the single most important limitation of this organism, and any honest recommendation has to start there. Sunlight damages fungal propagules and reduces the fungus's ability to produce new spores. Irradiance with simulated sunlight reduced conidiogenesis in laboratory work, although adjuvant treatment substantially offset the loss [1] . Temperature governs the speed of germination and growth. It interacts with humidity: warm dry conditions are the worst combination, because they accelerate drying of the spray deposit without supporting infection. The seasonal pattern in the field follows directly. In Brazilian rubber plantations, natural infection of pest mites peaked from November to February — the rainy season — and fell below 5% in the dry season [12] . What is the best time of day or weather condition to apply Hirsutella thompsonii ? Early morning or late afternoon, into a humid period, with rain not imminent. Late afternoon application gives the deposit a full night of high humidity and dew before the next day's sun Early morning application also works where dew persists Avoid mid-day application in bright sun and low humidity Avoid spraying immediately before heavy rain, which washes inoculum off before it establishes In monsoon-influenced systems, applications before the onset of monsoon and after monsoon are the conventional pattern for perennial crops Prefer overcast, humid conditions over clear, dry ones Time of day matters, but it is second to humidity. A perfectly timed evening spray into a dry spell will still underperform. Can Hirsutella thompsonii be mixed with fungicides, acaricides or other crop-protection products? Treat fungicides as incompatible unless a specific test says otherwise. H. thompsonii is a fungus. A fungicide that controls plant pathogens will generally also affect it, and this is the most common way a biological acaricide application is wasted. For other products, published compatibility data specific to H. thompsonii is limited, and general findings from related fungi do not transfer reliably. In one study of acaropathogenic fungi in Israel, three species tolerated most locally used insecticides and acaricides with the notable exception of sulfur [10] — but that result was for Meira and Acaromyces species, not for H. thompsonii , and should not be read across. The honest position: compatibility should be confirmed under the intended tank-mix conditions before commercial use. Evaluate physical mixing, viability of the fungus after the expected contact period, plant safety and performance. Where a mix has not been tested, apply the products separately and leave an interval between them. The current product documentation states compatibility with biopesticides, biofertilizers and plant growth regulators, and incompatibility with chemical fertilizers and chemical pesticides. Follow that guidance and verify locally before scaling a new mix. Does Hirsutella thompsonii affect beneficial insects or predatory mites? The evidence separates into two parts, and the second is often left out. Beneficial insects. In honey bee studies, H. thompsonii was harmless to the bees at the concentrations applied and had no deleterious effect on queen fecundity [13] . A separate study found it did not infect honey bees at larval, prepupal, pupal or adult stages under the rearing conditions used [7] . Predatory mites. Here there is a measurable effect. A formulated H. thompsonii product tested against the predatory mite Phytoseiulus longipes significantly reduced its net reproductive rate, intrinsic rate of increase and finite rate of increase. Under the IOBC toxicity classification the product was rated slightly harmful to adults, and residues became harmless to the predator around 10 days after spraying [16] . For comparison, in the same study four conventional insecticides were highly harmful to both eggs and adults and remained so past 31 days. The practical reading: H. thompsonii is markedly gentler on predatory mites than broad-spectrum chemistry, but it is not inert toward them. Where predatory mites are released as part of the program, allow roughly 10 days between application and release. Why can field performance of Hirsutella thompsonii vary between crops and seasons? Six reasons, most of which can be managed: Humidity and season. The dominant factor. Natural infection rates in one field system ranged from a rainy-season peak to under 5% in the dry season [12] , and a whole commercial program was judged unsuitable in one region because the local climate did not supply the humidity the fungus needs [10] Mite species. Susceptibility differs by family and by species, from highly susceptible eriophyids and tenuipalpids through to species that showed no infection at all [6] [14] Strain. Only three of nine isolates were infectious in one screen, and speed of kill varied nearly two-fold among those three [7] Coverage and mite habitat. A mite sheltered under a coconut perianth or on the underside of a dense canopy is much harder to reach than one on an exposed fruit surface Sunlight and formulation. Solar exposure reduces spore production, and adjuvants measurably offset that loss — one adjuvant raised conidium density by an average of 106% over control and boosted pathogenicity by 16.5% [1] Crop microclimate and surroundings. Management type and adjacent native vegetation both influenced infection rates in rubber plantations, with native vegetation acting as a reservoir and moderating the crop microclimate [11] The implication for a grower is that this product rewards attention to conditions. Applied into humid weather, at the right mite stage, with full coverage of the infested plant part, it performs very differently from the same product applied into a dry, sunny window. Sustainability Advantage Related Products Beauveria bassiana Isaria fumosorosea Lecanicillium lecanii Metarhizium anisopliae Nomuraea rileyi More Products Resources Read all

Lactobacillus reuteri promotes digestive health, supports immune function, and may reduce colic in infants, improving overall comfort. < Microbial Species Lactobacillus reuteri Lactobacillus reuteri promotes digestive health, supports immune function, and may reduce colic in infants, improving overall comfort. Strength 1 x 10⁸ CFU per gram / 1 x 10⁹ CFU per gram / 1 x 10¹⁰ CFU per gram Product Enquiry Buy this species Download Brochure Benefits Immune System Support It boosts immune function by increasing the production of antibodies and enhancing the body's defense against pathogens. Digestive Health Enhancement This probiotic supports gut health by promoting a balanced microbiota and alleviating symptoms of gastrointestinal discomfort. Antimicrobial Properties It produces substances that inhibit the growth of harmful bacteria, contributing to a healthier gut environment. Colic Relief in Infants This strain has been shown to alleviate colic symptoms in infants, promoting better digestive comfort. Dosage & Application Additional Info Scientific References Mode of Action Sustainability Advantage FAQ Scientific References Content coming soon! Mode of Action Content coming soon! Additional Info Key Features All microbial strains are characterized using 16S rDNA. All products are non-GMO. No animal-derived materials are used. The typical shelf life is 2 years. All strains are screened in-house using high-throughput screening methods. We can customize manufacturing based on the required strength and dosage. High-resilience strains Stable under a wide pH range Stable under a broad temperature range Stable in the presence of bile salts and acids Do not show antibiotic resistance Packaging Material The product is packaged in a multi-layer, ultra-high barrier foil that is heat-sealed and placed inside a cardboard shipper or plastic drum. Shipping Shipping is available worldwide. Probiotic packages are typically transported in insulated Styrofoam shippers with dry ice to avoid exposure to extreme high temperatures during transit. Support Documentation Certificate of Analysis (COA) Specifications Material Safety Data Sheets (MSDS) Stability studies (18 months) Certifications ISO 9001 ISO 22000 HACCP Halal and Kosher Certification (for Lactobacillus strains) FSSAI Dosage & Application Contact us for more details FAQ Content coming soon! Sustainability Advantage Related Products Bifidobacterium animalis Bifidobacterium bifidum Bifidobacterium breve Bifidobacterium infantis Bifidobacterium longum Clostridium butyricum Lactobacillus acidophilus Lactobacillus bulgaricus More Products Resources Read all

< Animal Health Aqua Pro AquaPro (Feed Supplement) is specially formulated probiotic product forshrimp, prawns, and fish Product Enquiry Benefits Regulates Harmful Microorganisms Maintains microbial balance in the gut by controlling the growth of pathogenic intestinal organisms, ensuring better biosecurity in the culture environment. Improves Growth and Productivity Increases the size and yield of aquatic species by enhancing overall performance and optimizing health and nutrition. Enhances Nutrient Absorption and Digestion Improves the utilization of proteins and nutrients from feed, supports a healthy gut, and promotes better digestion and feed intake. Boosts Immunity and Disease Prevention Strengthens the immune system and helps prevent the emergence of diseases, particularly bacterial infections, in fish, shrimp, and prawns. Component Amount per 30 mL Thiamine HCl 5 mg Ferrous Chloride 80 mg Nicotinic Acid 20 mg Nicotinamide 45 mg Ferrous Gluconate 200 mg Riboflavin 5 mg Calcium Lactate 300 mg Silymarin 100 mg Liver Fraction (II) (from 3.75g fresh liver) 150 mg Aqueous Base 30 mg Composition Distinction FAQ Additional Info FAQ Content coming soon! Dosage & Application Content coming soon! Additional Info Content coming soon! Related Products Piscicare Livcare Aquatract Aqua Energy Aquamin Probio Aqua More Products Resources Read all