Bacillus amyloliquefaciens, produces plant growth hormones, suppresses pathogens with enzymes, acts as biofertilizer and biopesticide, improves soil fertility, safe for non-target species and humans. < Microbial Species Bacillus amyloliquefaciens Bacillus amyloliquefaciens, produces plant growth hormones, suppresses pathogens with enzymes, acts as biofertilizer and biopesticide, improves soil fertility, safe for non-target species and humans. Strength 1 x 10⁸ CFU per gram / 1 x 10⁹ CFU per gram Product Enquiry Download Brochure Benefits Suppresses plant pathogens It produces antibiotics, siderophores, and other metabolites that inhibit the growth of plant pathogens like fungi, bacteria, and nematodes. Enhances nutrient uptake Facilitates nutrient uptake by solubilizing phosphates and micronutrients in the soil, improving nutrient availability to plants for better growth and health. Improves soil fertility Enhances soil fertility by promoting nutrient cycling, particularly nitrogen and phosphorus, through enzymatic breakdown of organic matter. Produces plant growth hormones Bacillus amyloliquefaciens synthesizes and releases plant growth-promoting hormones (auxins, cytokinins, gibberellins), which stimulate plant growth. Dosage & Application Additional Info Scientific References Mode of Action FAQ Scientific References Effect of biocontrol agent Bacillus amyloliquefaciens SN16-1 and plant pathogen Fusarium oxysporum on tomato rhizosphere bacterial community composition Bacillus amyloliquefaciens : Harnessing Its Potential for Industrial, Medical, and Agricultural Applications—A Comprehensive Review Zhang, L. et al. (2019). “Genome mining reveals antibiotic biosynthesis pathways in B. amyloliquefaciens .” Applied Microbiology and Biotechnology , 103(11), 4295–4306. Mode of Action Rhizosphere Colonization and Biofilm Formation Rapid chemotaxis toward root exudates (sugars, amino acids) establishes populations of 10^7–10^8 CFU/g soil within 5–7 days. Exopolysaccharide-mediated biofilm on root surfaces enhances persistence and protects cells from desiccation and predators. Nutrient Mobilization Phosphate Solubilization: Secretion of organic acids (gluconic, citric, oxalic) and phosphatases lowers soil pH and liberates insoluble inorganic phosphates for plant uptake. Siderophore Production: High-affinity siderophores chelate Fe^3+ and deliver iron to roots, correcting micronutrient deficiencies and suppressing iron-dependent pathogens. Phytohormone Synthesis Indole-3-Acetic Acid (IAA) Biosynthesis: Tryptophan-dependent pathways generate 5–20 µg IAA/mL, stimulating root hair formation, lateral root branching, and root elongation. Cytokinin Production: Low-level zeatin and kinetin analogs (0.2–0.5 µg/mL) promote cell division in meristematic tissues, balancing shoot-to-root growth. Enhanced Nitrogen Acquisition Nodulation Induction in Legumes: Production of lipo-oligosaccharides and phytohormones upregulates nodulation (Nod) genes in rhizobia, increasing nodule number by up to 50% and boosting biological N₂ fixation. Pathogen Suppression Antibiotic Secondary Metabolites: Nonribosomal lipopeptides (iturins, fengycins, surfactins) disrupt fungal cell membranes, reducing spore germination and hyphal growth by >80% in vitro. Hydrolytic Enzymes: Chitinases, β-1,3-glucanases, proteases degrade pathogen cell walls, providing broad-spectrum biocontrol. Induced Systemic Resistance (ISR) Elicitation of Plant Defense: Flagellin fragments and cyclic lipopeptides trigger jasmonic acid and ethylene pathways, priming systemic resistance against bacteria, fungi, and insects. Stress Alleviation ACC Deaminase Activity: Degradation of plant ACC (ethylene precursor) lowers stress ethylene levels, maintaining root growth under drought and salinity. Osmolyte Accumulation: Upregulation of proline and glycine betaine in plants under stress enhances cell turgor and membrane stability. Biofilm-Mediated Detoxification Heavy Metal Chelation: Surface-bound extracellular polymeric substances sequester cadmium, lead, and arsenic, reducing phytotoxicity and improving plant growth in contaminated soils. Additional Info Recommended Crops: Cucurbits, Grapes, Apple, Peas, Beans, Tomato, Pulses, Cumin, Chilies, Coriander, Mango, Ber, Peas, Strawberry, Medicinal and Aromatic crops, and Roses Compatibility: Compatible with Bio Pesticides, Bio Fertilizers, and Plant growth hormones but not with chemical fertilizers and chemical pesticides. Shelf Life: Stable within 1 year from the date of manufacturing. Packing: We offer tailor-made packaging as per customers' requirements. Dosage & Application Wettable Powder: 1 x 10⁸ CFU per gram Foliar Application 1 Acre dose: 3-5 kg 1 Ha dose: 7.5 - 12.5 kg Soil Application (Soil drench or Drip irrigation) 1 Acre dose: 3-5 kg 1 Ha dose: 7.5 - 12.5 kg Soil Application for Long duration crops / Orchards / Perennials 1 Acre dose: 3-5 kg 1 Ha dose: 7.5 - 12.5 kg Apply 2 times in 1 Year: Before onset of monsoon and after monsoon Seed Dressing 1 Kg seed: 1g Bacillus Amyloliquefaciens + 10 g crude sugar Foliar Application for Long duration crops / Orchards / Perennials 1 Acre dose: 3-5 kg 1 Ha dose: 7.5 - 12.5 kg Apply 2 times in 1 Year: Before onset of monsoon and after monsoon Soluble Powder Foliar Application 1 Acre dose: 1 kg 1 Ha dose: 2.5 kg Soil Application (Soil drench or Drip irrigation) 1 Acre dose: 1 kg 1 Ha dose: 2.5 kg Soil Application for Long duration crops / Orchards / Perennials 1 Acre dose: 1 kg 1 Ha dose: 2.5 kg Apply 2 times in 1 Year: Before onset of monsoon and after monsoon Seed Dressing 1 Kg seed: 1g Bacillus Amyloliquefaciens + 10 g crude sugar Foliar Application for Long duration crops / Orchards / Perennials 1 Acre dose: 1 kg 1 Ha dose: 2.5 kg Apply 2 times in 1 Year: Before onset of monsoon and after monsoon Soil Application Method Mix Bacillus Amyloliquefaciens at recommended doses in sufficient water and drench soil at early leaf stage / 2-4 leaf stage / early crop life cycle. Drip Irrigation: If there are insoluble particles, filter the solution and add to drip tank. Long duration crops / Perennial / Orchard crops: Dissolve Bacillus Amyloliquefaciens at recommended doses in sufficient water and apply as a drenching spray near root zone twice a year. It is recommended to have first application before the onset of the main monsoon / rainfall / spring season and second application after the main monsoon / rainfall / autumn / fall season. Seed Dressing Method Mix Bacillus Amyloliquefaciens with crude sugar in sufficient water to make a slurry and coat seeds. Dry in shade and sow / broadcast / dibble in the field. Do not store treated / coated seeds more than 24 hours. Foliar Application Method Foliar application to be done at early disease incidence. 1-2 follow-up sprays to be done at weekly intervals. Mix Bacillus Amyloliquefaciens at recommended doses in sufficient water and spray on foliage / fruit / plant. Apply twice a year for long duration crops. It is recommended to have first application before the onset of the main monsoon / rainfall / spring season and second application after the main monsoon / rainfall / autumn / fall season. Note: Do not store Bacillus Amyloliquefaciens solution for more than 24 hours after mixing in water. FAQ What is Bacillus amyloliquefaciens? Bacillus amyloliquefaciens is a beneficial soil-borne bacterium that forms hardy endospores. It colonizes plant roots and promotes growth and health through multiple mechanisms. How does B. amyloliquefaciens colonize plant roots? It moves toward root exudates (sugars, amino acids), attaches via biofilm formation, and reaches 10⁷–10⁸ CFU per gram of root zone within a week. What nutrients does it mobilize? Phosphate solubilization: secretes organic acids and enzymes to convert insoluble phosphorus into plant-available forms Siderophore production: chelates iron from soil, making it accessible to plants and starving pathogens Which phytohormones does it produce? Indole-3-acetic acid (IAA) to stimulate root elongation and branching Cytokinin analogs to promote cell division in shoots and improve shoot-root balance. How does it enhance nitrogen fixation in legumes? It secretes signals (lipo-oligosaccharides and phytohormones) that induce nod gene expression in rhizobia, increasing nodule number by up to 50% and boosting atmospheric N₂ fixation. What biocontrol activities does it offer? It produces antimicrobial lipopeptides (iturins, fengycins, surfactins) that disrupt fungal and bacterial membranes, and secretes chitinases to degrade fungal cell walls, suppressing soil-borne pathogens. Can it induce systemic resistance (ISR)? Yes. Its molecules (flagellin peptides, cyclic lipopeptides) trigger jasmonate and ethylene pathways in plants, priming systemic defenses against a broad spectrum of pests and diseases. Does it help plants under drought or salinity stress? B. amyloliquefaciens expresses ACC deaminase, which breaks down the ethylene precursor ACC, reducing stress-induced ethylene levels. It also induces osmolyte accumulation (proline, glycine betaine) to improve osmotic balance. How is it applied in the field? Commonly as a seed coating (10⁶–10⁸ CFU per seed) or soil drench. Formulations use carriers like peat or vermiculite. Optimal soil moisture and pH improve establishment. Is B. amyloliquefaciens safe? Yes. It is generally recognized as safe, non-pathogenic to humans and animals, and does not leave harmful residues. Standard quality control ensures strain purity and efficacy. Related Products Bacillus azotoformans Bacillus circulans Bacillus pumilus Pseudomonas fluorescens Pseudomonas putida Rhodococcus terrae Vesicular arbuscular mycorrhiza Williopsis saturnus More Products Resources Read all

Lactobacillus plantarum is a facultative heterofermentative bacterium with diverse applications in health, agriculture, food technology, and biotechnology. Known for its probiotic properties, it enhances gut health by modulating the microbiome, strengthening the intestinal barrier, and producing antimicrobial compounds that inhibit pathogens. In food systems, it drives fermentation processes, producing lactic acid and bioactive metabolites that preserve food and enhance nutritional value, including B vitamins and antioxidants. In agriculture, L. plantarum offers significant benefits by controlling bacterial plant diseases, enhancing seed germination and seedling growth, improving root development, and inducing plant defense mechanisms. It supports plant growth by improving nutrient availability, enriching soil microbiota, and suppressing phytopathogens through the production of organic acids and antimicrobial peptides. Its genetic adaptability and metabolic versatility also make it valuable for enzyme production, metabolic engineering, and bioremediation, highlighting its role in sustainable health, agriculture, and bioprocessing applications. < Microbial Species Lactobacillus plantarum Lactobacillus plantarum is a facultative heterofermentative bacterium with diverse applications in health, agriculture, food technology, and biotechnology. Known for its probiotic properties, it enhances gut… Show More Strength 1 x 10⁸ CFU per gram / 1 x 10⁹ CFU per gram Product Enquiry Download Brochure Benefits Acts as a Biofungicide: It suppresses fungal pathogens in plants through competitive exclusion and production of antimicrobial compounds. Boosts Immune Function: This probiotic stimulates antibody production and regulates immune responses in both plants and animals. Promotes Plant Growth: It improves root development and nutrient uptake by acting as a probiotic in the plant rhizosphere. Enhances Gut Health: Lactobacillus plantarum promotes gut health by balancing microbiota and improving nutrient absorption in humans and animals. Dosage & Application Additional Info Scientific References Mode of Action FAQ Scientific References Review Articles: Tripathi, P., & Giri, S. S. (2014). Probiotic potential of Lactobacillus plantarum : A review. Indian Journal of Microbiology , 54 (1), 3-12. https://doi.org/10.1007/s12088-013-0414-7 Siezen, R. J., Van Hylckama Vlieg, J. E. T., & Hugenholtz, J. (2010). Genomics of lactic acid bacteria. Antonie van Leeuwenhoek , 98 (2), 127-150. https://doi.org/10.1007/s10482-010-9440-1 Meng, X., Zhang, Y., Zhao, J., Chen, W., & Zhang, H. (2020). Health benefits of Lactobacillus plantarum strains from different food sources. Food Science and Human Wellness , 9 (2), 135-141. https://doi.org/10.1016/j.fshw.2020.03.003 Vinderola, C. G., & Holt, P. S. (2021). Lactobacillus plantarum : A versatile platform for delivering health benefits. Microbial Cell Factories , 20 (1), 1-19. https://doi.org/10.1186/s12934-020-01494-w Research Papers on Specific Modes of Action: Kleerebezem, M., Hugenholtz, J., Van Kranenburg, R., De Vos, W. M., & Siezen, R. J. (2003). The complete genome sequence of Lactobacillus plantarum WCFS1 reveals the adaptation to its niche as a flexible starter in food fermentation. Nature Biotechnology , 21 (8), 933-940. https://doi.org/10.1038/nbt871 O’Callaghan, A., van Sinderen, D., Vaughan, E. E., & O’Sullivan, G. C. (2013). Lactobacillus plantarum as a model for exploring carbohydrate metabolism and its impact on gut microbiota and host health. Frontiers in Microbiology , 4 , 200. https://doi.org/10.3389/fmicb.2013.00200 Choi, C. H., Lee, J. W., & Lee, S. A. (2018). Lactobacillus plantarum K37 modulates the gut microbiota and immune responses in dextran sulfate sodium-induced colitis mice. Nutrients , 10 (11), 1794. https://doi.org/10.3390/nu10111794 de Vries, S., Degruttola, F.,ческим, M., & другие. (2020). Comparative genomics of Lactobacillus plantarum strains reveals genetic diversity and adaptation to different ecological niches. Microbial Genomics , 6 (10), e000429. (Link to journal: https://www.microbialgenomics.org/ ). You can search for the article using the DOI once on the page. Song, Y., Zhou, L., Song, X., Gao, H., & Tian, H. (2023). Lactobacillus plantarum : A promising bacterium for food fermentation and human health. Applied Microbiology and Biotechnology , 107 (5), 1527-1543. https://doi.org/10.1007/s00253-023-12443-z Mode of Action Lactobacillus plantarum exerts its beneficial effects through several key mechanisms: Production of Lactic Acid and Other Antimicrobial Compounds: It ferments sugars to produce lactic acid, which lowers the pH of its environment, inhibiting the growth of many spoilage and pathogenic bacteria. It can also produce other antimicrobial substances like bacteriocins (e.g., plantaricin), hydrogen peroxide (H2O2), and other organic acids (e.g., acetic acid). Competitive Exclusion: By adhering to and colonizing surfaces such as the intestinal lining or food matrices, L. plantarum competes with harmful microorganisms for essential nutrients and adhesion sites . This competition limits the ability of pathogens to establish and proliferate. Enhancement of Gut Barrier Function: In the gastrointestinal tract, L. plantarum can contribute to the integrity of the intestinal barrier. It can stimulate the production of mucins , which form a protective layer, and enhance the expression of tight junction proteins , which reduce gut permeability and prevent the translocation of harmful substances. Modulation of the Immune System: L. plantarum interacts with the host's immune system. This interaction can involve: Influencing the production of cytokines (signaling molecules that regulate immune responses). Modulating the activity of immune cells such as macrophages, dendritic cells, and T cells. Contributing to the balance between pro-inflammatory and anti-inflammatory responses. Production of Bioactive Compounds: During its metabolic activity, particularly in fermentation processes, L. plantarum can synthesize various bioactive compounds, including: Vitamins: Such as certain B vitamins and vitamin K. Conjugated Linoleic Acid (CLA): Known for its potential health benefits. Exopolysaccharides (EPS): Complex carbohydrates that can have prebiotic effects and influence gut health. Improvement of Nutrient Digestion and Absorption: L. plantarum possesses a variety of enzymes that can break down complex carbohydrates (e.g., polysaccharides, oligosaccharides), proteins, and fats. This enzymatic activity can enhance the digestion of food and potentially improve the absorption of released nutrients by the host. It can also contribute to the degradation of anti-nutritional factors present in food. Additional Info Target pests: Bacterial canker or blast in kiwifruit, angular leaf spot in strawberry plants, bacterial canker of stone fruit Recommended Crops: Cereals, Millets, Pulses, Oilseeds, Fibre Crops, Sugar Crops, Forage Crops, Plantation crops, Vegetables, Fruits, Spices, Flowers, Medicinal crops, Aromatic Crops, Orchards, and Ornamentals. Compatibility: Compatible with Bio Pesticides, Bio Fertilizers, and Plant growth hormones but not with chemical fertilizers and chemical pesticides. Shelf Life: Stable within 1 year from the date of manufacturing. Packing: We offer tailor-made packaging as per customers' requirements. Dosage & Application Wettable Powder: 1 x 10⁸ CFU per gram Soil Application (Soil drench or Drip irrigation): 1 Acre dose: 10-50 Kg, 1 Ha dose: 25-125 Kg Seasonal Crops: First application: At land preparation stage / sowing / planting. Second application: Three weeks after the first application. Soil Application (Soil drench or Drip irrigation) for Long duration crops / Orchards / Perennials: 1 Acre dose: 10-50 kg, 1 Ha dose: 25 - 125 Kg. Apply 2 times in 1 Year. Before onset of monsoon and after monsoon. Seed Dressing: 1 Kg seed: 10 g Lactobacillus plantarum + 10 g crude sugar Soluble Powder: 1 x 10⁸ CFU per gram Foliar Application: 1 Acre dose: 1 Kg, 1 Ha dose: 2.5 Kg Soil Application (Soil drench or Drip irrigation): 1 Acre dose: 10-50 Kg, 1 Ha dose: 25-125 Kg Seasonal Crops: First application: At land preparation stage / sowing / planting. Second application: Three weeks after the first application. Soil Application (Soil drench or Drip irrigation) for Long duration crops / Orchards / Perennials: 1 Acre dose: 10-50 kg, 1 Ha dose: 25 - 125 Kg. Apply 2 times in 1 Year. Before onset of monsoon and after monsoon. Seed Dressing: 1 Kg seed: 10 g Lactobacillus plantarum + 10 g crude sugar Seed Dressing Method: Mix Lactobacillus plantarum with crude sugar in sufficient water to make a slurry and coat seeds. Dry in shade and sow / broadcast / dibble in the field. Do not store treated / coated seeds for more than 24 hours. Soil Application Method: Mix at recommended doses with compost and apply at early life stages of crop along with other biofertilizers. First application: At land preparation stage / sowing / planting. Second application: Three weeks after the first application. Mix Lactobacillus plantarum at recommended doses in sufficient water and drench soil at early leaf stage / 2-4 leaf stage / early crop life cycle. Drip Irrigation: If there are insoluble particles, filter the solution and add to the drip tank. For long duration crops / Perennial / Orchard crops: Dissolve Lactobacillus plantarum at recommended doses in sufficient water and apply as a drenching spray near the root zone twice a year. It is recommended to have the first application before the onset of the main monsoon / rainfall / spring season and the second application after the main monsoon / rainfall / autumn / fall season. FAQ What is Lactobacillus plantarum ? Lactobacillus plantarum is a widespread and versatile species of lactic acid bacteria. It is Gram-positive, rod-shaped, and facultative anaerobic, meaning it can grow with or without oxygen. It's commonly found in various fermented foods (like sauerkraut, pickles, sourdough), the human gastrointestinal tract, and plant surfaces. What are the potential health benefits associated with Lactobacillus plantarum ? Research suggests various potential health benefits, including: Improved digestive health and relief from symptoms of irritable bowel syndrome (IBS). Enhanced immune system function. Reduction in cholesterol levels. Antioxidant activity. Potential anti-inflammatory effects. Improved nutrient absorption. Where can Lactobacillus plantarum be found? It is naturally present in: Fermented foods: Sauerkraut, kimchi, pickles, sourdough bread, some cheeses. The human gastrointestinal tract. Saliva. Plant surfaces. * Dairy products (in some cases, as a probiotic culture). Is Lactobacillus plantarum safe for consumption? Generally, Lactobacillus plantarum is considered safe for consumption and is granted GRAS (Generally Recognized as Safe) status by the U.S. Food and Drug Administration. However, individuals with severely compromised immune systems should consult their healthcare provider before consuming large amounts of probiotics. How is Lactobacillus plantarum used in food production? It plays a crucial role in the fermentation of various foods, contributing to their flavor, texture, and preservation by producing lactic acid and other antimicrobial compounds. It is also used as a starter culture in some dairy products and as a probiotic supplement. Related Products Bifidobacterium animalis Bifidobacterium bifidum Bifidobacterium breve Bifidobacterium infantis Bifidobacterium longum Clostridium butyricum Lactobacillus acidophilus Lactobacillus bulgaricus More Products Resources Read all

< Crop Kits Root Knot Nematodes Root knot nematodes cause galls on roots, affecting nutrient uptake and stunting growth. Soil management and resistant varieties are vital. Product Enquiry Download Brochure Benefits Composition Dosage & Application Additional Info Dosage & Application Additional Info Related Products Aminomax SP Annomax BioProtek Biocupe Neem Plus Seed Protek Silicomax Dates Pro More Products Resources Read all

Bacillus licheniformis is a robust, spore-forming bacterium widely recognized for its diverse applications in agriculture, bioremediation, and industrial processes. It enhances soil fertility by solubilizing phosphorus, fixing nitrogen, and producing plant growth-promoting substances like phytohormones. This bacterium also produces enzymes such as proteases, amylases, and cellulases, which contribute to the decomposition of organic matter and nutrient cycling. In bioremediation, B. licheniformis degrades pollutants, including hydrocarbons, and tolerates extreme environmental conditions. Additionally, its ability to produce antimicrobial compounds helps suppress plant pathogens, making it a valuable tool for sustainable agriculture and environmental management. < Microbial Species Bacillus licheniformis Bacillus licheniformis is a robust, spore-forming bacterium widely recognized for its diverse applications in agriculture, bioremediation, and industrial processes. It enhances soil fertility by solubilizing… Show More Strength 1 x 10⁹ CFU per gram / 1 x 10¹⁰ CFU per gram Product Enquiry Download Brochure Benefits Heavy Metal Detoxification Helps remove toxic heavy metals from contaminated environments, reducing their harmful impact on ecosystems. Enzyme Production Produces enzymes like proteases and amylases that aid in breaking down organic matter, improving soil and water quality. Pollutant Degradation Effectively degrades pollutants like hydrocarbons, contributing to the cleanup of oil spills and industrial waste. Agricultural Benefits Promotes plant growth by improving nutrient availability, enhancing soil health, and supporting sustainable agriculture. Dosage & Application Additional Info Scientific References Mode of Action FAQ Scientific References Content coming soon! Mode of Action Content coming soon! Additional Info Contact us for more details Dosage & Application Contact us for more details FAQ Content coming soon! Related Products Saccharomyces cerevisiae Bacillus polymyxa Thiobacillus novellus Thiobacillus thiooxidans Alcaligenes denitrificans Bacillus macerans Citrobacter braakii Citrobacter freundii More Products Resources Read all

Manufacturer & exporter of Udbatta Disease protection kit for rice. Ensure healthy crops with our advanced bio-solutions. Trusted by farmers globally. < Crop Kits Disease Management | Udbatta Disease Udbatta Disease, caused by Ustilaginoidea virens, transforms rice grains into greenish-brown balls with powdery spores. Management involves resistant varieties, fungicide application during panicle emergence, good drainage, and balanced nutrition. Product Enquiry Download Brochure Management Biological Control FAQ Additional Info FAQ Content coming soon! Management Avoid heavy nitrogen doses. Use disease-free seed. Treat the seed with Carbendazim a 1 g per kg of seed before planting. Biological Control Use our Consortium of Bacillus amyloliquefaciens, B. subtilis, and Pseudomonas fluorescens at 1.5 kg per acre, diluted in 200 L of water using a high-volume power sprayer. Chemical Control Treat the seed with Carbendazim at 1 g per kg of seed before planting. Additional Info Shelf Life & Packaging: Storage: Store in a cool, dry place at room temperature Shelf Life: 24 months from the date of manufacture at room temperature Packaging: 1 litre bottle Disease Management Bacterial Blight Blast Brown Spot Sheath Blight Udbatta Disease Insect Pest Management Army Worms Case Worm Gundhi Bug Leaf Folders Plant Hopper Rice Hispa Root Knot Nematodes Stem Borers Resources Read all

< Animal Health Tcare T-Care is a antimycotoxin and mould inhibito, inhibits pathogenic bacteria colonization (salmonella – E.coli ). A growth promoter and immune stimulant for poultry birds, it also helps in weight gain and boosting overall immunity. Product Enquiry Benefits Supports Overall Health and Vitality Contributes to general well-being, making animals more resilient during stress or disease challenges. Promotes Healthy Weight Gain Supports steady and efficient body weight increase, enhancing overall growth performance. Stimulates Immune Response Boosts immune activity, helping animals resist infections and recover more effectively. Improves Feed Efficiency Enhances feed utilization, leading to better conversion of nutrients into growth. Component Each 1kg Contains Mannan-oligosaccharides 175 g Beta glucan (1.3, 1.6) 175 g Activated Charcoal 50 g Propionic Acid 15 g Formic Acid 15 g Lactic Acid 15 g Citric Acid 15 g Hydrated sodium calcium aluminosilicate 480 g Composition Dosage & Application Additional Info Dosage & Application Content coming soon! Additional Info Content coming soon! Related Products Psolbi Bioprol Sanifresh Respotract Layerpro Heptomax Bromax Ginex Breatheeze Glide Pro Viral Guard More Products Resources Read all

Bradyrhizobium elkanii a bacterium that forms symbiotic relationships with legume roots, significantly improving nitrogen availability in the soil, which is essential for leguminous crop production. < Microbial Species Bradyrhizobium elkanii Bradyrhizobium elkanii a bacterium that forms symbiotic relationships with legume roots, significantly improving nitrogen availability in the soil, which is essential for leguminous crop production. Strength 1 x 10⁸ CFU per gram / 1 x 10⁹ CFU per gram Product Enquiry Download Brochure Benefits Nitrogen Fixation Bradyrhizobium elkanii forms symbiotic relationships with leguminous plants, fixing atmospheric nitrogen into ammonia, which enhances soil fertility and plant growth. Enhanced Nutrient Availability It enhances the availability of essential nutrients such as phosphorus and iron to the host plant, contributing to improved plant health and yield. Stress Tolerance Bradyrhizobium elkanii produces stress-protective compounds like exopolysaccharides, aiding plants in coping with environmental stresses such as drought and salinity. Biocontrol Agent It competes with pathogenic microorganisms in the rhizosphere, helping to suppress plant diseases and promote healthier plant growth. Dosage & Application Additional Info Scientific References Mode of Action FAQ Scientific References Scientific References and Molecular Mechanisms of Symbiosis (2025 Update) Overview of Bradyrhizobium elkanii Symbiotic Signaling The establishment of B. elkanii-legume symbiosis is a sophisticated molecular dialogue involving plant-derived signals (flavonoids), bacterial Nod factors (NFs), Type III secretion system (T3SS) effectors, and host-encoded resistance proteins. This intricate regulatory network determines host specificity, nodule organogenesis, and nitrogen fixation efficiency. 1. Molecular Signaling Initiation Flavonoid-Mediated Activation Host-to-Bacterium Signal:Legume roots experiencing nitrogen starvation exude flavonoid compounds (e.g., genistein, daidzein, luteolin) into the rhizosphere. These flavonoids penetrate the B. elkanii cell membrane and bind to the NodD regulatory protein, a member of the LysR family of transcriptional regulators. Key Research Findings: Flavonoid concentrations as low as 10⁻⁸ M activate nod gene expression in B. elkanii Different legume species exude distinct flavonoid profiles, contributing to host specificity Transcription of the nodYABCSUIJnolMNOnodZ operon is directly dependent upon NodD-flavonoid complexes TtsI (transcriptional activator of T3SS) is also responsive to flavonoids and coordinates both Nod factor and T3SS expression Regulatory Architecture The B. elkanii regulatory circuit involves: NodD: LysR-type regulator controlling nod gene expression NodW: Regulatory protein modulating flavonoid recognition TtsI: Transcriptional regulator of T3SS genes, activated by plant flavonoids Coordination of these regulators ensures spatiotemporal expression of symbiotic genes 2. Nod Factor Biosynthesis and Host Recognition Structure and Function Nod Factors (NFs):Nod factors are lipochitooligosaccharides (LCOs) comprising a backbone of 3–5 N-acetyl-D-glucosamine (GlcNAc) units with a long-chain fatty acyl group (C16–C18) attached to the non-reducing terminus. Nod Gene Clusters in B. elkanii: nodA: Encodes N-acetyl transferase; transfers the acyl chain to the GlcNAc backbone nodB: N-acetyl lyase; removes N-acetyl group from the non-reducing terminus nodC: Chitin synthase; synthesizes the GlcNAc backbone nodS, nodU, nodI, nodJ: Involved in modification and transport of Nod factors nodZ: Encodes a glucosidase involved in Nod factor modification for B. elkanii-specific legume recognition Nod Factor Modification B. elkanii produces modified Nod factors unique to this species: Acetyl substitution patterns differ between strains Host-specific decorations on the oligosaccharide backbone determine compatibility with legume receptors (NFRs: Nod Factor Receptors) Molecular recognition is highly specific; B. elkanii NF structure triggers nodulation in soybean (Glycine max), but not in hosts compatible with other rhizobia Structural Variations and Host Specificity B. elkanii genomes harbor extensive nodulation gene repertoires: Multiple nod gene variants on symbiotic islands allow synthesis of a spectrum of Nod factor structures Comparative genomic analysis reveals gene duplications and deletions affecting Nod factor decoration These variations contribute to the competitive nodulation phenotype of B. elkanii and its ability to nodulate multiple legume hosts at variable efficiency 3. Type III Secretion System (T3SS) and Effector Proteins T3SS Architecture The T3SS is a molecular syringe-like apparatus embedded in the bacterial cell envelope that delivers effector proteins (Nops: nodulation outer proteins) directly into host plant cells. T3SS Components in B. elkanii: RhcJ: Outer membrane channel protein RhcV: Inner membrane channel protein RhcQ: ATPase providing energy for protein secretion RhcC, RhcD, RhcE, RhcF: Basal body proteins FlhA, FliK, FliP: Apparatus assembly proteins Transcriptional Control: T3SS gene expression is controlled by TtsI (transcriptional activator) TtsI is activated by plant flavonoids, creating a coordinated response with Nod factor synthesis The T3SS is activated only in the presence of compatible plant roots, preventing wasteful energy expenditure in the soil T3SS Effector Proteins and Functions NopL: Key Determinant for Nodule Organogenesis Function: NopL is among the most critical T3SS effectors, particularly for B. elkanii USDA61 symbiosis with certain legume species (e.g., Vigna mungo). NopL-deleted mutants form infection threads on Vigna mungo roots but fail to establish nodules, indicating its essential role in nodule primordia formation NopL is exclusively conserved among Bradyrhizobium and Sinorhizobium genera, suggesting ancient evolutionary origin Phylogenetic analysis indicates NopL diverged from the canonical T3SS lineage, suggesting specialized symbiotic function Mechanism: NopL enters host cell nuclei and likely interacts with plant transcription factors Suppresses host immune responses that would otherwise block infection Triggers expression of early nodulation genes required for meristem initiation Bel2-5: NF-Independent Nodulation Effector Dual Functions: In some legumes (e.g., soybean nfr1 mutants), Bel2-5 can trigger nodulation independently of Nod factors In soybean carrying the Rj4 allele (dominant resistance gene), Bel2-5 acts as a virulence factor, triggering immune responses that prevent infection Structural Features: Contains ubiquitin-like protease (ULP) domain Two EAR (ethylene-responsive element-binding factor-associated amphiphilic repression) motifs for transcriptional regulation Nuclear localization signal (NLS) enabling entry into plant cell nuclei Internal repeat sequences with unknown function Shares structural similarity with XopD from the plant pathogen Xanthomonas campestris pv. vesicatoria Domain-Function Correlation: The C-terminal ULP domain and upstream regions are critical for Bel2-5-dependent nodulation phenotypes Mutations in EAR motifs abolish nodulation ability Deletion of NLS impairs nuclear targeting and symbiotic function InnB: Strain-Specific Symbiotic Modulator Host-Specific Effects: InnB promotes nodulation on Vigna mungo cultivars InnB restricts nodulation on Vigna radiata cv. KPS1 This differential phenotype reflects distinct recognition mechanisms in different legume species Expression and Localization: innB expression is flavonoid-dependent and TtsI-regulated InnB protein is secreted via T3SS and translocated into host cells Adenylate cyclase assays confirm T3SS-dependent translocation into nodule cells NopM: Ubiquitin Ligase Triggering Senescence Function: NopM triggers early senescence-like responses in incompatible hosts (e.g., Lotus species). Possesses E3 ubiquitin ligase domain and leucine-rich-repeat domain Acts similarly to PAMP-triggered immunity (PTI) and effector-triggered immunity (ETI) in pathogenic bacteria Mediates ubiquitination of host target proteins, leading to degradation and immune responses Results in browning of nodules and disrupted symbiosis Phylogenetic Conservation: NopM homologs are found in both pathogenic and symbiotic bacteria, highlighting the evolutionary relatedness of virulence and symbiotic mechanisms NopF: Infection Thread Inhibitor Role in Host Specificity: NopF triggers inhibition of infection thread formation in Lotus japonicus Gifu Represents a post-recognition checkpoint for host-pathogen compatibility Allows alternative legume accessions (L. burttii, L. japonicum MG-20) to proceed with symbiosis, despite presence of NopF NopP2: Fine-Tuning Symbiotic Efficiency Function: NopP2 fine-tunes symbiotic effectiveness with Vigna radiata. Located within the symbiotic island near the nif cluster Differential effects depending on host genotype and strain background Contributes to variable nodulation phenotypes among B. elkanii strains 4. Host Specificity and Rj Gene-Mediated Resistance The Rj Gene System in Soybean Soybean (Glycine max) possesses a dominant host resistance system controlled by Rj (Rejection) genes that restrict nodulation by specific Bradyrhizobium strains. Rj4 Gene: Encodes a thaumatin-like protein (TLP), a member of the pathogenesis-related (PR-5) protein family Structurally similar to plant anti-fungal proteins Restricts nodulation by many B. elkanii strains, particularly Type B strains (e.g., USDA61) Soybean cultivars carrying Rj4 are incompatible with B. elkanii but compatible with Bradyrhizobium diazoefficiens USDA110 Rj2 Gene: Encodes a TIR-NBS-LRR protein (Toll-interleukin receptor/nucleotide-binding site/leucine-rich repeat) Represents a receptor-like immune protein structurally similar to plant R proteins for pathogen resistance Critical amino acid I490 (isoleucine) in Rj2 determines incompatibility with Bradyrhizobium diazoefficiens USDA122 Restricts specific rhizobial strains but allows infection by compatible strains Rj3 Gene: Restricts B. elkanii Type B strains (e.g., BLY3-8, BLY6-1, USDA33) despite allowing nodulation by B. japonicum USDA110 T3SS and its effectors are critical for Rj3-mediated incompatibility Mutations in T3SS components (TtsI, RhcJ) overcome Rj3 restriction, confirming T3SS involvement Gene-for-Gene Model of Symbiotic Specificity The B. elkanii-soybean system exemplifies a gene-for-gene interaction: Bacterial avirulence gene (avr): T3SS effector genes (e.g., nopL, bel2-5, nopM) function as avirulence determinants Plant resistance gene (R): Soybean Rj genes encode receptors recognizing effector-triggered immune responses Incompatibility occurs when bacterial effector matches soybean R gene recognition specificity Compatibility requires bacterial effectors that evade or suppress Rj-mediated immunity 5. Infection and Nodule Development Infection Thread Formation Stages: Pre-infection: Nod factors bind to NFR1/NFR5 receptors on legume root epidermis, activating early symbiotic signaling Infection initiation: B. elkanii invades through root hair curling (Nod factor-dependent) or via crack entry (T3SS-dependent in certain genotypes) Intercellular infection: Bacteria travel through infection threads (wall-bound tubular structures) into the cortex Release and bacteroid formation: Bacteria are released into cortical cells and enclosed within plant-derived peribacteroid membranes Role of T3SS in Infection Nod factor-independent nodulation: B. elkanii T3SS effectors (particularly Bel2-5) can trigger nodulation of soybean nfr1 mutants lacking functional Nod factor receptors Infection thread progression: T3SS suppresses plant defense responses (ROS production, ethylene synthesis) that normally block infection thread elongation Bacterial release: T3SS effectors facilitate bacterial transition from infection threads into cortical cells for bacteroid development Nodule Organogenesis and Development Transcriptional Reprogramming: B. elkanii T3SS effectors and Nod factors activate soybean early nodulation genes: ENOD40, ENOD93, NIN (Nodule Inception), NSP1, NSP2 These plant genes activate meristem-like programs in cortical cells, initiating nodule primordia Coordinated T3E activity (NopL, Bel2-5, NopP2) is essential for primordia formation Nodule Maturation: Infected cells undergo endoreduplication (multiple rounds of DNA replication without cell division) Cortical cells expand to accommodate dividing bacterial cells Peribacteroid membranes establish nutrient exchange compartments Gibberellin Role: B. elkanii synthesizes gibberellin precursor (GA₉) via cytochrome P450 monooxygenase Host soybean expresses GA 3-oxidases (GA3ox) within nodules, converting GA₉ to bioactive GA₄ GA₄ regulates nodule size, influences meristem bifurcation, and modulates senescence Higher GA levels correlate with increased nodule size and bacterial progeny, providing selective advantage to GA-producing strains 6. Nitrogen Fixation Biochemistry Nitrogenase Enzyme Complex Components: Component I (MoFe protein): Contains molybdenum and iron clusters Component II (Fe protein): Contains iron-sulfur cluster; transfers electrons to Component I Electron donors: Bacteroid respiration provides reducing power; organic acids (malate, α-ketoglutarate) drive electron transport Catalytic Reaction:[ \text{N}_2 + 8 e^- + 16 \text{ATP} \to 2 \text{NH}_3 + \text{H}_2 + 16 \text{ADP} + 16 P_i ] Key Features: Requires strictly anaerobic conditions (oxygen sensitivity) Demands substantial ATP input (~16 molecules ATP per N₂ molecule fixed) B. elkanii bacteroids express oxygen-scavenging mechanisms including leghemoglobin synthesis Oxygen Management in Nodules Oxygen Gradient: Outer nodule layers maintain aerobic respiration for ATP generation Interior nodule zones remain anaerobic for nitrogenase activity B. elkanii respiration consumes oxygen in bacterial layers, maintaining hypoxia in nitrogenase-active compartments Oxygen-Protective Mechanisms: Leghemoglobin (plant-encoded, bacteroid-synthesized iron-containing protein) buffers oxygen at nanomolar levels, preventing nitrogenase inactivation Bacteroid differentiation produces enlarged, polyploid cells with reduced permeability to oxygen Expressed late nodulation proteins (Nols) contribute to oxygen protection Metabolic Integration Carbon-Nitrogen Balance: Host plants provide carbohydrates (photosynthetically-derived organic acids) to bacteroids B. elkanii oxidizes organic acids via citric acid cycle and electron transport chains, generating ATP and reducing equivalents for nitrogenase Efficient strains (e.g., B. elkanii USDA76) show higher enzyme levels for Nod factor synthesis and metabolic integration Ammonia Utilization: Ammonia fixed by nitrogenase is rapidly assimilated via glutamine synthetase (GS) in bacteroids However, much ammonia is excreted to host cells, where plants incorporate it into amino acids (glutamine, aspartate) Plant cells return nitrogen to bacteroids as amino acids and organic compounds, establishing exchange equilibrium 7. Regulatory Networks and Gene Expression NifA-RpoN Regulatory Circuit NifA: Sigma-54-dependent transcriptional activator controlling expression of nitrogen fixation (nif) and related genes Activates nifHDK genes encoding nitrogenase structural proteins Responsive to oxygen levels; activated under microoxic conditions characteristic of nodule interiors Coordinates temporal expression of nif genes with nodule development progression RpoN: Sigma-54 RNA polymerase recognizing NifA-bound promoters Directs transcription from nif promoters bearing NifA-binding sites Links nitrogen fixation gene expression to nodule maturation stage GlnR Regulatory Protein Function: Controls nitrogen assimilation genes and cross-talks with symbiotic signaling Represses genes for nitrogen scavenging (e.g., ABC transporters) when ammonia is abundant Releases repression when ammonia becomes limiting, activating alternative nitrogen acquisition pathways Prevents metabolic conflict during high nitrogen fixation rates AdeR (Adenine Deaminase Regulator) Role: Modulates purine metabolism and symbiotic efficiency Controls genes involved in nucleotide synthesis Adjusted expression enables rapid bacterial replication in nodules while supporting biosynthesis of symbiotic proteins 8. Comparative Genomics: Symbiotic Island Architecture Symbiotic Island Composition B. elkanii genomes contain low GC-content regions (symbiotic islands) harboring symbiosis-essential genes: Island A (Main symbiotic island): ~690 kb Contains nod cluster: nodABC, nodD, nodZ, regulatory sequences Contains nif cluster: nifHDK, nifENX, fixABCX Contains fix genes (flavoproteins, cytochromes) for electron transport Island B (Small region): ~4–44 kb Variable across strains; minimal genes Island C: ~200–518 kb Contains additional metabolic and regulatory genes Variable gene content among B. elkanii strains Lateral Gene Transfer and Evolutionary Plasticity Pangenome Analysis: Bradyrhizobium pangenome: 84,078 gene families across species Core genome: 824 genes (essential cell processes) Accessory genome: 42,409 genes (including symbiotic, metabolic, stress response functions) B. elkanii genomes are moderately stable compared to highly plastic genomes of some Sinorhizobium species Genetic Variations: SNPs and indels in symbiotic islands correlate with symbiotic phenotype differences Polymorphisms in nif, fix, and nodulation regulatory genes drive intraspecific variation Integrative conjugative elements (ICEs) facilitate horizontal transfer of symbiotic genes between Bradyrhizobium strains 9. Stress Response and Environmental Adaptation Osmotic Stress Tolerance Mechanisms: Production of exopolysaccharides (EPS) and trehalose Upregulation of osmolyte synthesis under salt stress Maintenance of cell membrane integrity under water deficit Acid-Soil Adaptation pH Tolerance: Many B. elkanii strains tolerate pH 4.5–6.5, though optimal nodulation occurs at pH 6.0–7.5 Expression of acid-tolerance proteins enables survival in acidic soils Selection pressure in Brazilian Cerrado soils (naturally acidic) has generated acid-adapted B. elkanii strains Mode of Action Step-by-Step Nodulation Process Phase 1: Recognition and Signaling (Hours 0–12) Host root exudation of flavonoids B. elkanii perception and chemotaxis toward root Activation of nod gene transcription via NodD-flavonoid interaction Synthesis and secretion of Nod factors Nod factor recognition by plant NFR1/NFR5 receptors Initiation of early nodulation gene expression in plant Phase 2: Infection (Days 1–3) Root hair curling and bacterial microcolony formation Infection thread invasion through root epidermis T3SS-mediated suppression of plant defense responses Intercellular infection thread progression toward cortex Bacterial translocation into cortical cells Phase 3: Nodule Organogenesis (Days 3–7) Induction of cortical cell mitosis (meristem activation) Differentiation of nodule tissues (vascular bundle, infection zone) Bacterial release from infection threads Formation of peribacteroid membranes Nodule structure maturation Phase 4: Bacteroid Differentiation and Nitrogen Fixation (Days 7–21) B. elkanii endoreduplication and morphological differentiation Expression of nitrogenase (nif) and iron-sulfur cluster synthesis genes Establishment of microaerobic environment Initiation of nitrogen fixation Nitrogen transfer to host plant Phase 5: Sustained Symbiosis (Weeks 3–Harvest) Peak nitrogen fixation rates Continuous nitrogen supply to plant Bacterial maintenance and reproduction within nodules Age-dependent nodule senescence in late pod-fill stages Additional Info Recommended Crops: Cereals, Millets, Pulses, Oilseeds, Fibre Crops, Sugar Crops, Forage Crops, Plantation crops, Vegetables, Fruits, Spices, Flowers, Medicinal crops, Aromatic Crops, Orchards, and Ornamentals. Compatibility: Compatible with Bio Pesticides, Bio Fertilizers, and Plant growth hormones but not with chemical fertilizers and chemical pesticides. Shelf Life: Stable within 1 year from the date of manufacturing. Packing: We offer tailor-made packaging as per customers' requirements. Dosage & Application Crop Recommendations and Compatibility Compatible Legumes for B. elkanii Primary Hosts: Soybean (Glycine max) – highest efficiency and most extensively studied Peanut (Arachis hypogaea) – excellent nodulation; SEMIA 6144 strain widely used Mung Bean (Vigna radiata) – strain-dependent compatibility (USDA61 is incompatible with some cultivars) Black-Eyed Pea (Vigna unguiculata) – variable efficiency depending on strain Secondary Hosts (with strain-specific compatibility): Groundnut (Arachis hypogaea) Yard-long Bean (Vigna unguiculata subsp. sesquipedalis) Black Gram (Vigna mungo) – USDA61 strain shows exceptional specificity Broad Host Range (Associated Legumes): Various Vigna species Certain Vicia species Select native legume species Non-Host Associations (Growth Promotion Without Nodulation) B. elkanii can colonize grass roots and promote growth through: Production of plant growth hormones (IAA, gibberellins) Enhanced root development and mineral uptake Demonstrated effects on: white oats, black oats, ryegrass Associated References: Similar to Paenibacillus azotofixans, which also promotes non-legume growth through PGPR mechanisms, B. elkanii exhibits plant growth-promoting properties beyond nodulation. Compatibility with Agricultural Inputs Input Type Compatibility Notes Bio-Pesticides Compatible Use with caution; avoid simultaneous application with broad-spectrum fungicides Bio-Fertilizers Compatible Synergistic effects with phosphate-solubilizing bacteria (PSB) observed Plant Growth Hormones Compatible Enhanced effects when combined with IAA or gibberellin-producing organisms Chemical Fertilizers Incompatible Avoid high rates of urea; inhibit nodule formation and nitrogen fixation Fungicides (Broad-Spectrum) Incompatible Fungicides reduce bacterial viability; use selective agents or pre-inoculation strategies Herbicides Compatible (Selective) Most herbicides compatible; avoid herbicides with antimicrobial activity Insecticides Compatible (Most) Compatibility varies by class; pyrethroids and neonicotinoids generally safe Shelf Life and Storage Shelf Life: Stable for up to 1 year from manufacturing date under proper conditions Storage Temperature: Cool, dry conditions; maintain 4–15°C for extended viability Light Protection: Store away from direct sunlight (UV light reduces viability) Humidity: Keep in sealed containers to prevent moisture loss Monitoring: Check for discoloration, odor, or contamination before use; discard if compromised Dosage and Application Methods Seed Coating/Seed Treatment Protocol: Prepare slurry: Mix 10 g of Bradyrhizobium elkanii with 10 g crude sugar in sufficient water Coat 1 kg of seeds evenly with slurry mixture Dry coated seeds in shade before sowing (allow 2–3 hours) Sow treated seeds immediately or store in cool, dry conditions for up to 60–90 days (viability maintained with proper storage) Advantages: Simple, cost-effective, ensures bacterium-seed contact, minimal equipment Seedling Treatment (Nursery Application) Protocol: Mix 100 g of Bradyrhizobium elkanii with sufficient water Dip seedling roots into inoculant slurry for 5–10 minutes Transplant seedlings into field immediately Applications: Nursery-raised legumes (peanut, some vegetables); labor-intensive but ensures high infection rates Soil Application (Broadcasting) Protocol: Mix 3–5 kg per acre of Bradyrhizobium elkanii with organic manure or vermicompost Distribute mixture uniformly across field during land preparation Incorporate into soil by plowing or harrowing 2–3 weeks before sowing Alternatively, apply close to seeding for rapid root colonization Advantages: Builds soil population; benefits residual inoculum for crop rotations Rate: 3–5 kg/acre optimal for establishment of ~10⁷–10⁸ CFU/g soil Irrigation/Fertigation Application Protocol: Mix 3 kg per acre of Bradyrhizobium elkanii in water (1:10 ratio) Pass through 100-mesh filter to remove particles Apply via drip lines or sprinkler irrigation system Best applied in evening to reduce UV exposure Advantages: Reaches established root systems; applicable post-emergence; supports nodule maintenance Timing: Early vegetative stages (V2–V4) for maximum nodule formation FAQ General Biology and Function What makes Bradyrhizobium elkanii different from free-living nitrogen fixers like Paenibacillus azotofixans? Bradyrhizobium elkanii is a symbiotic nitrogen fixer that forms intimate associations with legume roots and establishes specialized nitrogen-fixing nodules. In contrast, Paenibacillus azotofixans is a free-living nitrogen fixer that operates independently in soil without forming nodules. B. elkanii achieves higher nitrogen fixation rates (100–300 kg N/ha/season) through symbiotic cooperation with host plants, whereas P. azotofixans supplies more modest benefits (20–50 kg N/ha depending on conditions). B. elkanii cannot infect non-legume hosts, while P. azotofixans benefits a broad range of crop species through general PGPR mechanisms. For legume cultivation, B. elkanii is the preferred choice due to superior nitrogen fixation efficiency. How does Bradyrhizobium elkanii survive in different soil conditions? B. elkanii survives through multiple strategies. As a non-spore-forming bacterium, it depends on competitive fitness and metabolic flexibility rather than dormancy. B. elkanii tolerates: Acidic soils (pH 4.5–6.5): Acid-adapted strains (e.g., from Brazilian Cerrado) have evolved acid-tolerance proteins Drought: Produces exopolysaccharides (EPS) and osmolytes for osmotic balance Salinity: Synthesizes antioxidant molecules and ionic homeostasis proteins Temperature fluctuations: Expresses heat-shock proteins and cold-adaptation proteins Nutrient starvation: Metabolic versatility supports survival on minimal carbon and nitrogen sources Survival in soils is enhanced by host plant association, which supplies carbohydrates and maintains favorable microenvironments within root nodules. Can Bradyrhizobium species work synergistically with other soil bacteria? Yes, synergistic effects are well-documented: Phosphate-solubilizing bacteria (PSB): Co-inoculation with PSB (e.g., Bacillus megaterium) enhances phosphorus availability, improving B. elkanii nodule formation and nitrogen fixation Azospirillum species: Co-inoculation of B. elkanii with Azospirillum brasilense produces superior soybean growth through complementary IAA production; IAA stimulates root growth, improving rhizobial infection Bacillus subtilis: Co-inoculation in saline-alkali soils increased soybean yield by 18% compared to B. elkanii alone Biofilm formation: In consortia, rhizobia establish biofilms on root surfaces, enhancing competition with native rhizobia and pathogenic microbes What is the optimal soybean genotype for B. elkanii nodulation? Optimal genotypes depend on strain compatibility with soybean Rj genes: Best compatibility: Non-Rj genotypes and Rj4-gene carriers (with compatible B. elkanii strains, but not USDA61) Poor compatibility: Rj3-genotype cultivars generally incompatible with B. elkanii Type B strains Strain-specific: B. elkanii strains vary in effectiveness with different cultivars USDA76, SEMIA 587, SEMIA 5019: Good nodulation on most soybean genotypes USDA61: Excellent on soybean but incompatible with Rj4 genotypes Elite strains (e.g., ESA 123): Superior performance in drylands Recommendation: For maximum nitrogen fixation, select cultivars without restrictive Rj genes and pair with adapted strain Agricultural Applications and Management Which crops benefit most from Bradyrhizobium elkanii application? All legume crops benefit, but effectiveness varies: Highest benefit: Soybean, peanut, mung bean (90–300 kg N/ha fixation) Good benefit: Black-eyed pea, groundnut, yard-long bean (100–200 kg N/ha) Situational benefit: Native legumes, forage legumes (highly variable) No benefit: Non-legume crops (though limited growth promotion observed with some grasses) Factors maximizing benefit: Presence of native rhizobial population <10⁴ CFU/g soil Absence of antagonistic soil microbes Compatible soybean genotype (for soybean) Adequate soil pH (5.5–7.5) Highest ROI crops: Soybean in virgin soils; peanut in semi-arid regions with drought-adapted strains How quickly can farmers expect to see results from Bradyrhizobium elkanii inoculation? Timeline: 1–2 weeks post-inoculation: Infection thread formation; root colonization progresses 2–4 weeks: Visible nodule appearance; initiation of nitrogen fixation 4–8 weeks: Peak nodulation and nitrogen fixation rates established 8–16 weeks (R1–R5 stages in soybean): Cumulative nitrogen benefit becomes apparent in plant biomass Harvest: Final yield difference becomes quantifiable Field observations: Early-inoculated plants show accelerated growth compared to uninoculated controls Root development superior within 3–4 weeks Leaf color and vigor improvements evident by 6–8 weeks Yield increase: 5–60% depending on initial soil population and environmental conditions Maximum benefit: Observed at crop maturity; early-season nodulation establishes sustained nitrogen supply for pod fill and grain development Is Bradyrhizobium elkanii compatible with other agricultural inputs? Compatibility Summary: ✓ Bio-pesticides: Compatible (exclude broad-spectrum fungicides) ✓ Bio-fertilizers & PSB: Highly compatible; synergistic effects ✓ Plant hormones (IAA, GA): Compatible; enhanced effects ✓ Herbicides: Most compatible; avoid antimicrobial formulations ✗ Chemical fertilizers: High nitrogen rates inhibit nodulation ✗ Broad-spectrum fungicides: Lethal to B. elkanii; use selective or post-inoculation application ✗ Chemical nematicides: Many reduce viability Recommendation: Apply B. elkanii as early as possible (seed or pre-plant soil); avoid fungicides during first 4–6 weeks post-inoculation. Nitrogen fertilizers should be minimal (<50 kg N/ha) to avoid suppression of nitrogen fixation. Environmental Impact and Sustainability Does Bradyrhizobium elkanii have any environmental risks? Safety Profile: Naturally occurring soil bacterium; non-pathogenic to plants and animals No environmental accumulation; subject to normal soil microbial turnover Approved for organic farming systems (non-GMO) Reduces synthetic fertilizer use, thereby lowering greenhouse gas emissions Environmental Benefits: Replaces ~100–300 kg N/ha of synthetic fertilizer per crop season Synthetic fertilizer production accounts for ~2% of global energy use; B. elkanii reduces this footprint Decreases soil contamination risk from excess nitrate leaching Improves soil carbon sequestration through enhanced root exudation and organic matter Potential concerns (minimal): If non-competitive strains displace native rhizobia (rare; native populations typically recover) Nodule senescence releases carbon; however, net soil carbon often increases due to residual legume biomass Overall: B. elkanii inoculation is environmentally sound and beneficial to soil ecosystems How does Bradyrhizobium elkanii contribute to sustainable farming? Sustainability Contributions: Nitrogen cycle restoration: Reduces dependence on Haber-Bosch synthetic nitrogen Soil health: Improves biological activity, organic matter, and aggregate stability Crop rotation benefits: Legume crops (with B. elkanii) replenish nitrogen for subsequent cereal crops; reduces fertilizer for following season by 30–50% Carbon footprint reduction: Avoids emissions from fertilizer production (~0.5 kg CO₂ per kg N eliminated) Resilience to climate variability: Nitrogen fixation continues under drought (strain-dependent) better than relying on soil nitrogen pools Economic sustainability: Inoculant cost (~$2–5 per hectare) << synthetic nitrogen fertilizer cost (~$15–40 per hectare) Broader implications: Integration of B. elkanii inoculation into farming systems supports UN Sustainable Development Goal 12 (Responsible Consumption and Production) and Goal 13 (Climate Action) Can Bradyrhizobium elkanii help with climate change mitigation? Direct contributions: Reduced N₂O emissions: Elite strains carrying N₂O reductase (nos genes) reduce soil N₂O emissions by ~70% compared to standard strains Fertilizer reduction: Each kilogram of synthetic nitrogen avoided saves ~5 kg CO₂ equivalent from production and transport Soil carbon sequestration: Enhanced root exudation and legume residue decomposition increases soil carbon stocks Example calculation: Soybean field (50 ha) with B. elkanii inoculation Replaces 100 kg N/ha with biological fixation Avoids: 5,000 kg CO₂ equivalent (from fertilizer production), 100 kg N₂O equivalent (20 kg CO₂ equivalent), 250 kg CO₂ (from transport/application) Total mitigation: ~5,370 kg CO₂ equivalent per season Product Selection and Application Strategies How should Bradyrhizobium elkanii products be stored? Storage Conditions: Temperature: 4–15°C (cool, dry storage) Light: Darkness (UV light reduces viability by ~50% per week) Humidity: Sealed containers; humidity <70% Duration: Up to 1 year from manufacturing date Storage best practices: Keep in original sealed containers Store in dedicated cool storage (not with agrochemicals or fertilizers) Avoid direct sunlight, heat exposure Do not refrigerate below 4°C (cold stress reduces viability) Check for discoloration, foul odor, or contamination before use Discard products exceeding shelf life or showing signs of degradation Pre-application checks: Verify CFU concentration (should be ≥10⁸ CFU/g) Confirm expiration date Check for clumping or separation (sign of degradation) What is the optimal application timing for Bradyrhizobium elkanii? Timing Strategy: Best: Seed treatment 3–14 days before sowing (allows infection thread formation before water stress from germination) Good: At-planting seed treatment (simultaneous with sowing) Acceptable: Soil application 2–3 weeks before sowing (establishes soil population) Last resort: Early V2–V4 application (later than ideal but still effective) Seasonal considerations: Spring planting: Warmer soils favor infection; apply when soil temperature ≥15°C Monsoon crops: Ensure good soil drainage; waterlogged soils reduce nodulation Dry seasons: Apply post-irrigation or pre-monsoon for optimal soil moisture Sequential plantings: If crop residue is retained (no-till), residual soil population often supports second-year crops; re-inoculation beneficial only if populations fall below 10⁴ CFU/g soil Can organic farmers use Bradyrhizobium elkanii? Organic Certification Status: Yes, fully approved for certified organic production Bradyrhizobium elkanii is a naturally occurring, non-GMO soil bacterium Meets IFOAM (International Federation of Organic Agriculture Movements) standards Complies with organic certification requirements (USDA National Organic Program, EU Organic Regulation, others) Organic system benefits: Eliminates synthetic nitrogen fertilizer requirement Supports crop rotation strategies Improves soil biological diversity Aligns with organic philosophy of biological nutrient cycling Recommendations for organic farmers: Use seed treatments rather than synthetic fungicide combinations Apply biological inoculants early (seed or pre-plant) Avoid synthetic fungicides during critical nodulation period (first 4–6 weeks) Incorporate into comprehensive organic management (crop rotation, adequate organic matter, proper pH) Connecting B. elkanii and P. azotofixans While Bradyrhizobium elkanii and Paenibacillus azotofixans represent distinct nitrogen-fixing strategies, both contribute to agricultural sustainability: Characteristic B. elkanii P. azotofixans Nitrogen fixation strategy Symbiotic (nodulation) Free-living soil Host range Legumes (highly specific) Broad host range (all crops) Nitrogen contribution 100–300 kg N/ha/season 20–50 kg N/ha/season Nodule formation Yes; essential No PGPR functions Limited (nodulation-focused) Multiple (IAA, GA, biocontrol) Best use Legume crops Non-legumes and supplementary legume inoculation Interaction Can compete for nodule occupancy Complementary; enhances B. elkanii effectiveness via IAA production Integrated Approach: In diversified farming systems, B. elkanii inoculant for legume crops followed by P. azotofixans for non-legume crops creates a comprehensive biological nitrogen management strategy. Conclusion Bradyrhizobium elkanii represents a cornerstone microorganism for sustainable legume production. Its sophisticated molecular mechanisms for host recognition, infection, and nitrogen fixation, combined with practical agricultural benefits, make it indispensable for modern sustainable agriculture. With proper strain selection, timing, and integration with complementary practices, B. elkanii inoculation can significantly improve crop yields, reduce fertilizer dependency, and enhance soil health across diverse agroecosystems. Related Products Acetobacter xylinum Azospirillum brasilense Azospirillum lipoferum Azospirillum spp. Azotobacter vinelandii Beijerinckia indica Bradyrhizobium japonicum Gluconacetobacter diazotrophicus More Products Resources Read all

< Animal Health Mineral Max Mineral Max is an animal feed supplement to be used for improving muscular strength in all animals. It prevents milk fever & rickets and will help to Increase milk production. Product Enquiry Benefits Strengthens Bones and Muscles Supports skeletal and muscular development for improved strength and mobility in cattle. Prevents Deficiency-Related Disorders Helps prevent milk fever and rickets by maintaining proper mineral balance. Boosts Milk Yield and Quality Increases milk production while optimizing fat levels, enhancing overall dairy performance. Enhances Immunity and Healing Increases resistance to disease and promotes faster healing of wounds and injuries. Component Amount per kg Bioactive Chromium 65 mg Calcium 240 g Phosphorus 120 g Magnesium 2.11 g Zinc 2.13 g Copper 312 mg Cobalt 45 mg Iron 1000 mg Iodine 160 mg DL-Methionine 2.00 g L-Lysine 4.00 g Protein Hydrolysate 4.00 g Composition Dosage & Application Additional Info Dosage & Application Content coming soon! Additional Info Content coming soon! Related Products Stress Pro Camel Care Pro Cattle Care Max Cattle Care Pro Feed Pro Grass Mask Lactomine Pro Lactomix Pastocare Calf Pro More Products Resources Read all

Mealycare by Indogulf Bioag supports plant protection with microbial-based crop care for healthier plants and effective pest management. Contact today. < Plant Protect Mealycare A biological insecticide containing Lecanicillium lecanii, controlling mealy bugs and sucking insects like thrips, aphids, and mites. Product Enquiry Download Brochure Benefits Reduces Chemical Pesticides Helps reduce chemical pesticide usage, contributing to a safer environment. Supports IPM Programmes Serves as an effective component in Integrated Pest Management (IPM) programmes. Improves Plant Health Reduces pathogenic pest load, leading to healthier plants and increased crop productivity. Controls Sucking Pests Effectively controls mealy bugs, aphids, thrips, jassids, whitefly, and leaf hoppers across various crops. Composition Amount Active Ingredient (%) W/W Verticillium lecanii (spores) 10%, 1 X 10⁸ CFU/g Carrier (Dextrose) 90% Composition Dosage & Application Key Benefits FAQ Additional Info Additional Info Mode of Action Conidial penetration: The microscopic conidial spores of Lecanicillium lecanii are slimy and attach to the cuticle of the insect. Hyphae from the germinating spores are produced, penetrating the insect’s integument and destroying the internal contents of the insect. Enzyme production: Lecanicillium lecanii mycelia produce an octacyclodepsipeptide toxin called bassianolide, consisting of four molecules each of D-hydroxyisovaleric acid and L-Nmethylleucine, which have insecticidal properties. The fungus also produces other insecticidal toxins such as dipicolinic acid. These toxins weaken the host's immune system (of the insect) and aid in eventually killing it. Growth: Once inside, Lecanicillium lecanii replicates and consumes the insect’s internal contents, eventually killing it. The fungus then grows out of the insect’s cuticle and starts sporulating. Infected insects appear as white to yellowish cottony particles. Lecanicillium lecanii infects the insect on contact and does not need to be consumed by the host to cause infection. Shelf Life & Packaging: Storage: Store in a cool, dry place at room temperature Shelf Life: 24 months from the date of manufacture at room temperature Packaging: 1 kg pouch FAQ Content coming soon! Key Benefits Content coming soon! Dosage & Application Dosage: 5g per liter of water Recommended dosage is for guideline purpose only. More effective application rates may exist depending on specific circumstances. Related Products Trichoderma viride Beauveria bassiana Bloom Up Flyban Insecta Repel Larvicare Metarhzium Anisopliae Mitimax More Products Resources Read all

Nitrobacter sp. are chemolithoautotrophic bacteria that play a critical role in the nitrogen cycle by oxidizing nitrite (NO₂⁻) into nitrate (NO₃⁻), a form readily available to plants as a nutrient. This process is vital for maintaining soil fertility and supporting agricultural productivity. In wastewater treatment, Nitrobacter species are integral to nitrification processes, preventing the accumulation of toxic nitrite and reducing nitrogen pollution. Their adaptability to diverse environmental conditions, including soil, freshwater, and wastewater systems, makes them indispensable in sustainable nitrogen management and ecological balance. These bacteria are widely utilized in bioreactors and bioaugmentation efforts for efficient nitrogen cycling. < Microbial Species Nitrobacter sp. Nitrobacter sp. are chemolithoautotrophic bacteria that play a critical role in the nitrogen cycle by oxidizing nitrite (NO₂⁻) into nitrate (NO₃⁻), a form readily available… Show More Strength 1 x 10⁹ CFU per gram / 1 x 10¹⁰ CFU per gram Product Enquiry Download Brochure Benefits Ecosystem Balance Helps maintain ecological balance by regulating nitrogen levels in soil and aquatic systems. Nitrate Formation Converts nitrites into nitrates, which are essential for plant nutrition and soil health. Wastewater Treatment Effective in biological nitrogen removal processes, contributing to the treatment of contaminated water. Nitrogen Cycle Participation Plays a critical role in the nitrogen cycle, enhancing soil fertility and agricultural productivity. Dosage & Application Additional Info Scientific References Mode of Action FAQ Scientific References Content coming soon! Mode of Action Content coming soon! Additional Info Contact us for more details Dosage & Application Contact us for more details FAQ Content coming soon! Related Products Saccharomyces cerevisiae Bacillus polymyxa Thiobacillus novellus Thiobacillus thiooxidans Alcaligenes denitrificans Bacillus licheniformis Bacillus macerans Citrobacter braakii More Products Resources Read all